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Article: Caveolin-1 overexpression is associated with hepatocellular carcinoma tumourigenesis and metastasis

TitleCaveolin-1 overexpression is associated with hepatocellular carcinoma tumourigenesis and metastasis
Authors
Keywordscancer metastasis
caveolin-1
hepatocellular carcinoma
tumourigenesis
Issue Date2012
PublisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130
Citation
Journal of Pathology, 2012, v. 226 n. 4, p. 645-653 How to Cite?
AbstractCaveolin-1 (Cav1) has been implicated in diverse human cancers, yet its role in hepatocellular carcinoma (HCC) tumourigenesis and metastasis remains elusive. In the current study, we aim to provide a comprehensive understanding regarding the functional role of Cav1 in HCC tumourigenesis and metastasis. Cav1 expression was examined in a panel of human HCC cell lines using western blotting analysis and quantitative RT-PCR and human tissues by immunohistochemistry. Cav1 was not detected in normal liver cell line and all non-tumourous liver tissues but exclusively expressed in HCC cell lines and tissues. Dramatic expression of Cav1 was found in metastatic HCC cell lines and tumours, indicating a progressive increase of Cav1 expression along disease progression. Cav1 overexpression was significantly correlated with venous invasion (p = 0.036). To investigate the functions of Cav1 in HCC, Cav1 overexpressing and knockdown stable clones were established in HCC cells and their tumourigenicity and metastatic potential were examined. Overexpression of Cav1 promoted HCC cell growth, motility, and invasiveness, as well as tumourigenicity in vivo. Conversely, knockdown of Cav1 in metastatic HCC cells inhibited the motility and invasiveness and markedly suppressed the tumour growth and metastatic potential in vivo. Collectively, our findings have shown the exclusive expression of Cav1 in HCC cell lines and clinical samples and revealed an up-regulation of Cav1 along HCC progression. The definitive role of Cav1 in promoting HCC tumourigenesis was demonstrated, and we have shown for the first time in a mouse model that Cav1 promotes HCC metastasis. © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/148682
ISSN
2015 Impact Factor: 7.381
2015 SCImago Journal Rankings: 4.176
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong
Hong Kong Research Grants CouncilHKU783310M
Collaborative Research FundHKU7/CRF/09
Funding Information:

This work was supported by The University of Hong Kong Seed Funding Programme for Basic Research and Outstanding Young Researcher Award (to JWPY), Hong Kong Research Grants Council General Research Fund (HKU783310M), and Collaborative Research Fund (HKU7/CRF/09). IOL Ng is Loke Yew Professor in Pathology.

References

 

DC FieldValueLanguage
dc.contributor.authorTse, EYTen_HK
dc.contributor.authorKo, FCFen_HK
dc.contributor.authorTung, EKKen_HK
dc.contributor.authorChan, LKen_HK
dc.contributor.authorLee, TKWen_HK
dc.contributor.authorNgan, ESWen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorWong, ASTen_HK
dc.contributor.authorNg, IOLen_HK
dc.contributor.authorYam, JWPen_HK
dc.date.accessioned2012-05-29T06:14:39Z-
dc.date.available2012-05-29T06:14:39Z-
dc.date.issued2012en_HK
dc.identifier.citationJournal of Pathology, 2012, v. 226 n. 4, p. 645-653en_HK
dc.identifier.issn0022-3417en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148682-
dc.description.abstractCaveolin-1 (Cav1) has been implicated in diverse human cancers, yet its role in hepatocellular carcinoma (HCC) tumourigenesis and metastasis remains elusive. In the current study, we aim to provide a comprehensive understanding regarding the functional role of Cav1 in HCC tumourigenesis and metastasis. Cav1 expression was examined in a panel of human HCC cell lines using western blotting analysis and quantitative RT-PCR and human tissues by immunohistochemistry. Cav1 was not detected in normal liver cell line and all non-tumourous liver tissues but exclusively expressed in HCC cell lines and tissues. Dramatic expression of Cav1 was found in metastatic HCC cell lines and tumours, indicating a progressive increase of Cav1 expression along disease progression. Cav1 overexpression was significantly correlated with venous invasion (p = 0.036). To investigate the functions of Cav1 in HCC, Cav1 overexpressing and knockdown stable clones were established in HCC cells and their tumourigenicity and metastatic potential were examined. Overexpression of Cav1 promoted HCC cell growth, motility, and invasiveness, as well as tumourigenicity in vivo. Conversely, knockdown of Cav1 in metastatic HCC cells inhibited the motility and invasiveness and markedly suppressed the tumour growth and metastatic potential in vivo. Collectively, our findings have shown the exclusive expression of Cav1 in HCC cell lines and clinical samples and revealed an up-regulation of Cav1 along HCC progression. The definitive role of Cav1 in promoting HCC tumourigenesis was demonstrated, and we have shown for the first time in a mouse model that Cav1 promotes HCC metastasis. © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130en_HK
dc.relation.ispartofJournal of Pathologyen_HK
dc.subjectcancer metastasisen_HK
dc.subjectcaveolin-1en_HK
dc.subjecthepatocellular carcinomaen_HK
dc.subjecttumourigenesisen_HK
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAnimalsen_US
dc.subject.meshCarcinoma, Hepatocellular - Genetics - Mortality - Secondaryen_US
dc.subject.meshCaveolin 1 - Genetics - Metabolismen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshCell Movementen_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshClone Cellsen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshDisease Progressionen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Knockdown Techniquesen_US
dc.subject.meshGene Silencingen_US
dc.subject.meshHong Kong - Epidemiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshLiver - Metabolism - Pathologyen_US
dc.subject.meshLiver Neoplasms - Genetics - Mortality - Pathologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred BALB Cen_US
dc.subject.meshMice, Nudeen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNeoplasm Transplantationen_US
dc.subject.meshSurvival Rateen_US
dc.subject.meshUp-Regulationen_US
dc.subject.meshYoung Adulten_US
dc.titleCaveolin-1 overexpression is associated with hepatocellular carcinoma tumourigenesis and metastasisen_HK
dc.typeArticleen_HK
dc.identifier.emailTse, EYT: sadietse@hkucc.hku.hken_HK
dc.identifier.emailKo, FCF: bokcf@hku.hken_HK
dc.identifier.emailTung, KK: edmund@pathology.hku.hken_HK
dc.identifier.emailChan, LK: lkchan1@hku.hken_HK
dc.identifier.emailLee, TKW: tkwlee@hkucc.hku.hken_HK
dc.identifier.emailNgan, ESW: engan@hku.hk-
dc.identifier.emailMan, K: kwanman@hku.hk-
dc.identifier.emailWong, AST: awong1@hkucc.hku.hk-
dc.identifier.emailNg, IOL: iolng@hku.hk-
dc.identifier.emailYam, JWP: judyyam@pathology.hku.hk-
dc.identifier.authorityLee, TKW=rp00447en_HK
dc.identifier.authorityNgan, ESW=rp00422en_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.identifier.authorityYam, JWP=rp00468en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/path.3957en_HK
dc.identifier.pmid22072235en_HK
dc.identifier.scopuseid_2-s2.0-84856724303en_HK
dc.identifier.hkuros197843-
dc.identifier.hkuros201926-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84856724303&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume226en_HK
dc.identifier.issue4en_HK
dc.identifier.spage645en_HK
dc.identifier.epage653en_HK
dc.identifier.eissn1096-9896-
dc.identifier.isiWOS:000299779300009-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTing Tse, EY=54881237000en_HK
dc.identifier.scopusauthoridFat Ko, FC=54881236900en_HK
dc.identifier.scopusauthoridKwan Tung, EK=54881236800en_HK
dc.identifier.scopusauthoridChan, LK=24833005000en_HK
dc.identifier.scopusauthoridWah Lee, TK=7501439435en_HK
dc.identifier.scopusauthoridWai Ngan, ES=22234827500en_HK
dc.identifier.scopusauthoridMan, K=7101754072en_HK
dc.identifier.scopusauthoridTsai Wong, AS=54881237300en_HK
dc.identifier.scopusauthoridNg, IOL=7102753722en_HK
dc.identifier.scopusauthoridPing Yam, JW=6603711123en_HK
dc.customcontrol.immutablesml 130620-

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