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Article: Mcl-1 expression in gestational trophoblastic disease correlates with clinical outcome: A differential expression study

TitleMcl-1 expression in gestational trophoblastic disease correlates with clinical outcome: A differential expression study
Authors
Keywordsc-DNA array
Gestational trophoblastic disease
Hydatidiform mole
Mcl-1
Issue Date2005
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741
Citation
Cancer, 2005, v. 103 n. 2, p. 268-276 How to Cite?
AbstractBACKGROUND. Hydatidiform moles (HMs) are abnormal pregnancies with a propensity for developing persistent disease in the form of gestational trophoblastic neoplasia (GTN), which requires chemotherapy. In previous studies, the authors demonstrated that low apoptotic activity was correlated with the progression of HM to GTN, and they hypothesized that some apoptosis-related genes may determine this progression. METHODS. The differential expression of apoptotic genes in HMs that subsequently developed into GTN was compared with the same expression in HMs that spontaneously regressed using a human apoptosis array; then, the expression was evaluated with real-time quantitative polymerase chain reaction analysis and immunohistochemistry using 54 clinical samples from patients with HMs who had follow-up data available. RESULTS. Using an apoptosis array, greater expression of Mcl-1, which is an antiapoptotic gene, was detected in HMs that subsequently developed into GTN. It was confirmed that the levels of Mcl-1 RNA expression (P = 0.017) and Mcl-1 protein expression (P < 0.001) in HMs that developed into persistent disease and required chemotherapy were significantly greater compared with the levels in HMs that regressed. Moreover, Mcl-1 immunoreactivity, which was detected predominantly in cytotrophoblasts, was correlated with the apoptotic index, as assessed with M30 cytoDeath immunohistochemistry, which is a good indicator of apoptotic events in the early-stage disease. CONCLUSIONS. The current results demonstrated that Mcl-1, as identified by a cyclic DNA array, may play a role in the pathogenesis of HMs and may have potential as a useful marker for predicting the clinical behavior of HMs. © 2004 American Cancer Society.
Persistent Identifierhttp://hdl.handle.net/10722/148384
ISSN
2014 Impact Factor: 4.889
2014 SCImago Journal Rankings: 2.510
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFong, PYen_HK
dc.contributor.authorXue, WCen_HK
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorChan, KYKen_HK
dc.contributor.authorKhoo, USen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorChiu, PMen_HK
dc.contributor.authorMan, LSen_HK
dc.contributor.authorCheung, ANYen_HK
dc.date.accessioned2012-05-29T06:12:38Z-
dc.date.available2012-05-29T06:12:38Z-
dc.date.issued2005en_HK
dc.identifier.citationCancer, 2005, v. 103 n. 2, p. 268-276en_HK
dc.identifier.issn0008-543Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/148384-
dc.description.abstractBACKGROUND. Hydatidiform moles (HMs) are abnormal pregnancies with a propensity for developing persistent disease in the form of gestational trophoblastic neoplasia (GTN), which requires chemotherapy. In previous studies, the authors demonstrated that low apoptotic activity was correlated with the progression of HM to GTN, and they hypothesized that some apoptosis-related genes may determine this progression. METHODS. The differential expression of apoptotic genes in HMs that subsequently developed into GTN was compared with the same expression in HMs that spontaneously regressed using a human apoptosis array; then, the expression was evaluated with real-time quantitative polymerase chain reaction analysis and immunohistochemistry using 54 clinical samples from patients with HMs who had follow-up data available. RESULTS. Using an apoptosis array, greater expression of Mcl-1, which is an antiapoptotic gene, was detected in HMs that subsequently developed into GTN. It was confirmed that the levels of Mcl-1 RNA expression (P = 0.017) and Mcl-1 protein expression (P < 0.001) in HMs that developed into persistent disease and required chemotherapy were significantly greater compared with the levels in HMs that regressed. Moreover, Mcl-1 immunoreactivity, which was detected predominantly in cytotrophoblasts, was correlated with the apoptotic index, as assessed with M30 cytoDeath immunohistochemistry, which is a good indicator of apoptotic events in the early-stage disease. CONCLUSIONS. The current results demonstrated that Mcl-1, as identified by a cyclic DNA array, may play a role in the pathogenesis of HMs and may have potential as a useful marker for predicting the clinical behavior of HMs. © 2004 American Cancer Society.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741en_HK
dc.relation.ispartofCanceren_HK
dc.subjectc-DNA arrayen_HK
dc.subjectGestational trophoblastic diseaseen_HK
dc.subjectHydatidiform moleen_HK
dc.subjectMcl-1en_HK
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBiopsy, Needleen_US
dc.subject.meshCohort Studiesen_US
dc.subject.meshDna, Complementary - Analysisen_US
dc.subject.meshDisease Progressionen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshGestational Trophoblastic Disease - Genetics - Pathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshHydatidiform Mole - Genetics - Pathologyen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIn Situ Nick-End Labelingen_US
dc.subject.meshLogistic Modelsen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNeoplasm Proteins - Genetics - Metabolismen_US
dc.subject.meshPregnancyen_US
dc.subject.meshProbabilityen_US
dc.subject.meshPrognosisen_US
dc.subject.meshProto-Oncogene Proteins C-Bcl-2 - Genetics - Metabolismen_US
dc.subject.meshRna, Neoplasm - Analysisen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshTumor Markers, Biological - Analysisen_US
dc.subject.meshUterine Neoplasms - Genetics - Pathologyen_US
dc.titleMcl-1 expression in gestational trophoblastic disease correlates with clinical outcome: A differential expression studyen_HK
dc.typeArticleen_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.emailChan, KYK: kelvinc@pathology.hku.hken_HK
dc.identifier.emailKhoo, US: uskhoo@hku.hken_HK
dc.identifier.emailTsao, SW: gswtsao@hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.authorityChan, KYK=rp00453en_HK
dc.identifier.authorityKhoo, US=rp00362en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1002/cncr.20767en_HK
dc.identifier.pmid15578716en_HK
dc.identifier.scopuseid_2-s2.0-12344276322en_HK
dc.identifier.hkuros109806en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-12344276322&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume103en_HK
dc.identifier.issue2en_HK
dc.identifier.spage268en_HK
dc.identifier.epage276en_HK
dc.identifier.isiWOS:000226379000008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridFong, PY=12242921700en_HK
dc.identifier.scopusauthoridXue, WC=7103165268en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK
dc.identifier.scopusauthoridChan, KYK=7406034195en_HK
dc.identifier.scopusauthoridKhoo, US=7004195799en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridChiu, PM=7103182596en_HK
dc.identifier.scopusauthoridMan, LS=16638079400en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK

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