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Article: Liver transplantation in rats using small-for-size grafts: A study of hemodynamic and morphological changes

TitleLiver transplantation in rats using small-for-size grafts: A study of hemodynamic and morphological changes
Authors
Issue Date2001
PublisherAmerican Medical Association. The Journal's web site is located at http://www.archsurg.com
Citation
Archives Of Surgery, 2001, v. 136 n. 3, p. 280-285 How to Cite?
AbstractBackground: Damage to a small-for-size liver graft after reperfusion is frequently observed but the mechanism of injury remains unclear. Hypothesis: Injury to a small-for-size liver graft is related to the changes of portal pressure and blood flow. Main Outcome Measures: Survival rates, portal hemodynamics, microcirculatory changes, and morphological changes (by light microscopy and electron microscopy). Setting: A rat model of nonarterialized orthotopic liver transplantation comparing 2 groups of rats transplanted with whole grafts (100% of recipient liver weight) and small-for-size grafts (30% of recipient liver weight). Results: Median survival of the rats with small-for-size grafts was 30 hours (range, 27-37 hours). During the first 15 minutes after reperfusion, mean arterial pressure of the small-for-size graft group was significantly lower than that of the whole graft group (10-minute: 100 vs 132 mm Hg, P=.04; 15-minute: 96 vs 127 mm Hg, P=.04). Portal pressure (in centimeters of water) of the small-for-size graft group was significantly higher in the first 20 minutes after reperfusion than the level before the an hepatic phase (5-minute: 15.1 vs 9.3, P=.02; 10-minute: 16.1 vs 9.3, P=.03: 15-minute, 13.5 vs 9.3, P=.03; 20-minute: 13.4 vs 9.3, P=.03) and was significantly higher than that of the whole graft group in the first 10 minutes after reperfusion (5-minute: 15.1 vs 9.6, P=.02; 10-minute: 16.1 vs 10.3, P=.04). Hepatic microcirculatory blood flow (in milliliters per minute per 100 g) was also significantly higher in the small-for-size graft group during the first 40 minutes after reperfusion (5-minute: 16.3 vs 9.3. P=.02: 10-minute: 14.9 vs 6.6, P=.02; 15-minute: 14.8 vs 5.5, P=.02; 20-minute: 13.1 vs 7.0, P=.02; 30-minute: 13.2 vs 8.8, P=.04; 40-minute: 14.6 vs 7.1, P=.02) Light and electron microscopy showed normal morphological features of whole graft up to 24 hours after reperfusion. The small-for-size graft, however, showed sinusoidal congestion, tremendous swelling of mitochondria of hepatocytes, irregular large gap of sinusoidal lining cells, and collapse of the space of Disse. Conclusions: In a rat model, the portal hemodynamic changes in small-for-size grafts are transient. Progressive damage of the graft may result from microcirculatory failure due to irreversible endothelial injury after reperfusion.
Persistent Identifierhttp://hdl.handle.net/10722/148233
ISSN
2014 Impact Factor: 4.926
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMan, Ken_HK
dc.contributor.authorLo, CMen_HK
dc.contributor.authorNg, IOLen_HK
dc.contributor.authorWong, YCen_HK
dc.contributor.authorQin, LFen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorWong, Jen_HK
dc.date.accessioned2012-05-29T06:11:40Z-
dc.date.available2012-05-29T06:11:40Z-
dc.date.issued2001en_HK
dc.identifier.citationArchives Of Surgery, 2001, v. 136 n. 3, p. 280-285en_HK
dc.identifier.issn0004-0010en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148233-
dc.description.abstractBackground: Damage to a small-for-size liver graft after reperfusion is frequently observed but the mechanism of injury remains unclear. Hypothesis: Injury to a small-for-size liver graft is related to the changes of portal pressure and blood flow. Main Outcome Measures: Survival rates, portal hemodynamics, microcirculatory changes, and morphological changes (by light microscopy and electron microscopy). Setting: A rat model of nonarterialized orthotopic liver transplantation comparing 2 groups of rats transplanted with whole grafts (100% of recipient liver weight) and small-for-size grafts (30% of recipient liver weight). Results: Median survival of the rats with small-for-size grafts was 30 hours (range, 27-37 hours). During the first 15 minutes after reperfusion, mean arterial pressure of the small-for-size graft group was significantly lower than that of the whole graft group (10-minute: 100 vs 132 mm Hg, P=.04; 15-minute: 96 vs 127 mm Hg, P=.04). Portal pressure (in centimeters of water) of the small-for-size graft group was significantly higher in the first 20 minutes after reperfusion than the level before the an hepatic phase (5-minute: 15.1 vs 9.3, P=.02; 10-minute: 16.1 vs 9.3, P=.03: 15-minute, 13.5 vs 9.3, P=.03; 20-minute: 13.4 vs 9.3, P=.03) and was significantly higher than that of the whole graft group in the first 10 minutes after reperfusion (5-minute: 15.1 vs 9.6, P=.02; 10-minute: 16.1 vs 10.3, P=.04). Hepatic microcirculatory blood flow (in milliliters per minute per 100 g) was also significantly higher in the small-for-size graft group during the first 40 minutes after reperfusion (5-minute: 16.3 vs 9.3. P=.02: 10-minute: 14.9 vs 6.6, P=.02; 15-minute: 14.8 vs 5.5, P=.02; 20-minute: 13.1 vs 7.0, P=.02; 30-minute: 13.2 vs 8.8, P=.04; 40-minute: 14.6 vs 7.1, P=.02) Light and electron microscopy showed normal morphological features of whole graft up to 24 hours after reperfusion. The small-for-size graft, however, showed sinusoidal congestion, tremendous swelling of mitochondria of hepatocytes, irregular large gap of sinusoidal lining cells, and collapse of the space of Disse. Conclusions: In a rat model, the portal hemodynamic changes in small-for-size grafts are transient. Progressive damage of the graft may result from microcirculatory failure due to irreversible endothelial injury after reperfusion.en_HK
dc.languageengen_US
dc.publisherAmerican Medical Association. The Journal's web site is located at http://www.archsurg.comen_HK
dc.relation.ispartofArchives of Surgeryen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshEndothelium, Vascular - Pathologyen_US
dc.subject.meshLiver - Blood Supply - Pathologyen_US
dc.subject.meshLiver Transplantation - Pathologyen_US
dc.subject.meshMitochondria, Liver - Pathologyen_US
dc.subject.meshOrgan Size - Physiologyen_US
dc.subject.meshPortal Pressure - Physiologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Sprague-Dawleyen_US
dc.subject.meshRegional Blood Flow - Physiologyen_US
dc.subject.meshReperfusion Injury - Pathologyen_US
dc.titleLiver transplantation in rats using small-for-size grafts: A study of hemodynamic and morphological changesen_HK
dc.typeArticleen_HK
dc.identifier.emailMan, K: kwanman@hku.hken_HK
dc.identifier.emailLo, CM: chungmlo@hkucc.hku.hken_HK
dc.identifier.emailNg, IOL: iolng@hku.hken_HK
dc.identifier.emailWong, YC: ycwong@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailWong, J: jwong@hkucc.hku.hken_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityLo, CM=rp00412en_HK
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.identifier.authorityWong, YC=rp00316en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityWong, J=rp00322en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid11231846-
dc.identifier.scopuseid_2-s2.0-0035097777en_HK
dc.identifier.hkuros56635-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035097777&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume136en_HK
dc.identifier.issue3en_HK
dc.identifier.spage280en_HK
dc.identifier.epage285en_HK
dc.identifier.isiWOS:000167368300004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMan, K=7101754072en_HK
dc.identifier.scopusauthoridLo, CM=7401771672en_HK
dc.identifier.scopusauthoridNg, IOL=7102753722en_HK
dc.identifier.scopusauthoridWong, YC=7403041798en_HK
dc.identifier.scopusauthoridQin, LF=7201950985en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK
dc.identifier.scopusauthoridWong, J=8049324500en_HK

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