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Article: Immunohistochemical and mutational analysis of p53 tumor suppressor gene in gestational trophoblastic disease: Correlation with mdm2, proliferation index, and clinicopathologic parameters

TitleImmunohistochemical and mutational analysis of p53 tumor suppressor gene in gestational trophoblastic disease: Correlation with mdm2, proliferation index, and clinicopathologic parameters
Authors
KeywordsGestational trophoblastic disease
Ki67
mdm2
p53
Issue Date1999
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.ijgc.net/
Citation
International Journal Of Gynecological Cancer, 1999, v. 9 n. 2, p. 123-130 How to Cite?
AbstractThe role of p53 in the pathogenesis of gestational trophoblastic disease (GTD) was investigated. Immunohistochemical studies for p53, its regulator mdm2, and proliferation marker Ki67 were performed on paraffin-embedded tissues of 28 partial moles (PM), 57 complete moles (CM), 14 choriocarcinomas (CCA), and 31 normal placentas. Three antibodies to p53 (DO-7, Ab-2, Ab-3) were used and demonstrated immunoreactivity for wild-type p53 protein predominantly in the nuclei of cytotrophoblasts. Direct DNA sequencing of 36 hydatidiform moles using frozen tissues confirmed an absence of mutational changes in exons 5-8. CCA was found to have the highest p53 protein expression, followed by CM, PM, and normal placenta (P < 0.001). In normal placentas (P = 0.0001), PM, and CM (P = 0.016), an inverse correlation between their gestational age and p53 expression was observed, p53 expression was found to correlate with proliferation index in normal placenta (P = 0.0001) and all three groups of GTD (P = 0.012). Significant correlation between p53 and mdm2 expression was also observed (P < 0.01). The distinctive expression of p53 wild-type protein in the cytotrophoblasts and its positive correlation with the proliferative index suggests that its overexpression in GTD may be related to its effect on cell proliferation. The parallel expression of mdm2 and p53 also supports the presence of an autoregulatory feedback loop in the control of this process. No correlation could be found between clinical progress of the patients with hydatidiform moles, and the p53 (P = 0.78) or mdm2 protein (P = 0.54) expression suggesting that neither of them carries any prognostic significance.
Persistent Identifierhttp://hdl.handle.net/10722/148154
ISSN
2015 Impact Factor: 2.116
2015 SCImago Journal Rankings: 0.830
References

 

DC FieldValueLanguage
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorShen, DHen_HK
dc.contributor.authorKhoo, USen_HK
dc.contributor.authorChiu, MPMen_HK
dc.contributor.authorTin, VPCen_HK
dc.contributor.authorChung, LPen_HK
dc.contributor.authorNgan, HYSen_HK
dc.date.accessioned2012-05-29T06:11:08Z-
dc.date.available2012-05-29T06:11:08Z-
dc.date.issued1999en_HK
dc.identifier.citationInternational Journal Of Gynecological Cancer, 1999, v. 9 n. 2, p. 123-130en_HK
dc.identifier.issn1048-891Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/148154-
dc.description.abstractThe role of p53 in the pathogenesis of gestational trophoblastic disease (GTD) was investigated. Immunohistochemical studies for p53, its regulator mdm2, and proliferation marker Ki67 were performed on paraffin-embedded tissues of 28 partial moles (PM), 57 complete moles (CM), 14 choriocarcinomas (CCA), and 31 normal placentas. Three antibodies to p53 (DO-7, Ab-2, Ab-3) were used and demonstrated immunoreactivity for wild-type p53 protein predominantly in the nuclei of cytotrophoblasts. Direct DNA sequencing of 36 hydatidiform moles using frozen tissues confirmed an absence of mutational changes in exons 5-8. CCA was found to have the highest p53 protein expression, followed by CM, PM, and normal placenta (P < 0.001). In normal placentas (P = 0.0001), PM, and CM (P = 0.016), an inverse correlation between their gestational age and p53 expression was observed, p53 expression was found to correlate with proliferation index in normal placenta (P = 0.0001) and all three groups of GTD (P = 0.012). Significant correlation between p53 and mdm2 expression was also observed (P < 0.01). The distinctive expression of p53 wild-type protein in the cytotrophoblasts and its positive correlation with the proliferative index suggests that its overexpression in GTD may be related to its effect on cell proliferation. The parallel expression of mdm2 and p53 also supports the presence of an autoregulatory feedback loop in the control of this process. No correlation could be found between clinical progress of the patients with hydatidiform moles, and the p53 (P = 0.78) or mdm2 protein (P = 0.54) expression suggesting that neither of them carries any prognostic significance.en_HK
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.ijgc.net/en_HK
dc.relation.ispartofInternational Journal of Gynecological Canceren_HK
dc.subjectGestational trophoblastic diseaseen_HK
dc.subjectKi67en_HK
dc.subjectmdm2en_HK
dc.subjectp53en_HK
dc.titleImmunohistochemical and mutational analysis of p53 tumor suppressor gene in gestational trophoblastic disease: Correlation with mdm2, proliferation index, and clinicopathologic parametersen_HK
dc.typeArticleen_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.emailKhoo, US: uskhoo@hkucc.hku.hken_HK
dc.identifier.emailChung, LP: lpchung@hkucc.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityKhoo, US=rp00362en_HK
dc.identifier.authorityChung, LP=rp00249en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1046/j.1525-1438.1999.09904.xen_HK
dc.identifier.scopuseid_2-s2.0-0032896883en_HK
dc.identifier.hkuros44183en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032896883&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.issue2en_HK
dc.identifier.spage123en_HK
dc.identifier.epage130en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridShen, DH=7401738584en_HK
dc.identifier.scopusauthoridKhoo, US=7004195799en_HK
dc.identifier.scopusauthoridChiu, MPM=7101865589en_HK
dc.identifier.scopusauthoridTin, VPC=6603199735en_HK
dc.identifier.scopusauthoridChung, LP=24315879100en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK

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