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  Patent History
  • Application
    US 12/192393 2008-08-15
  • Publication
    US 20090047680 2009-02-19
Supplementary

published patent: Methods And Compositions For High-Throughput Bisulphite Dna-Sequencing And Utilities

TitleMethods And Compositions For High-Throughput Bisulphite Dna-Sequencing And Utilities
Priority Date2008-08-15 US 12/192393
2007-08-15 US /935472P
2007-09-05 US /935867P
Inventors
Issue Date2009
Citation
US Published patent application 20090047680. Washington, DC: US Patent and Trademark Office (USPTO), 2009 How to Cite?
AbstractThe Invention Relates To Novel Methods And Compositions To Produce Dna Templates Suitable For Chemical Modifications And High-Throughput Dna-Sequencing. A Method Of The Invention Relates To A Dna Adaptor Design Where Constituent Deoxycytosines Are Substituted With 5-Methyl-Deoxycytosines Rendering The Resulting Adaptor Resistant To Bisulphite Mediated Deamination. When Said Adaptor Is Ligated Onto Double Stranded Dna Template, Subsequent Dna Denaturation And Bisulphite Treatment Deaminates Template Dna Deoxycytosine Differentially To Deoxyuraeil Whilst The 5-Methyl-Deoxycytosines Of The Ligated Adaptor Resist Chemical Conversion Resulting In The Adaptor Sequence Remaining Unaltered. Both Strands Of Bisulphite Treated Dna Can Thus Be Amplified With A Single Primer Set That Hybridizes To The Unaltered Adaptor Sequence. The Invention Also Relates To Methods To Produce Control Template Of A Defined Methylation Composition To Optimize Conditions For The Bisulphite Reaction. In A Preferred Embodiment, The Present Invention Can Be Used To Produce Templates Suitable For Genome-Wide Bisulphite-Dna Sequencing Using Conventional, Solexa(Tm), Solid(Tm) Or 454(Tm)-Type Dna Sequencing Platforms To Study Dna Methylation.
Persistent Identifierhttp://hdl.handle.net/10722/142242
References

 

DC FieldValueLanguage
dc.date.accessioned2011-10-19T06:36:12Z-
dc.date.available2011-10-19T06:36:12Z-
dc.date.issued2009-
dc.identifier.citationUS Published patent application 20090047680. Washington, DC: US Patent and Trademark Office (USPTO), 2009en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142242-
dc.description.abstractThe Invention Relates To Novel Methods And Compositions To Produce Dna Templates Suitable For Chemical Modifications And High-Throughput Dna-Sequencing. A Method Of The Invention Relates To A Dna Adaptor Design Where Constituent Deoxycytosines Are Substituted With 5-Methyl-Deoxycytosines Rendering The Resulting Adaptor Resistant To Bisulphite Mediated Deamination. When Said Adaptor Is Ligated Onto Double Stranded Dna Template, Subsequent Dna Denaturation And Bisulphite Treatment Deaminates Template Dna Deoxycytosine Differentially To Deoxyuraeil Whilst The 5-Methyl-Deoxycytosines Of The Ligated Adaptor Resist Chemical Conversion Resulting In The Adaptor Sequence Remaining Unaltered. Both Strands Of Bisulphite Treated Dna Can Thus Be Amplified With A Single Primer Set That Hybridizes To The Unaltered Adaptor Sequence. The Invention Also Relates To Methods To Produce Control Template Of A Defined Methylation Composition To Optimize Conditions For The Bisulphite Reaction. In A Preferred Embodiment, The Present Invention Can Be Used To Produce Templates Suitable For Genome-Wide Bisulphite-Dna Sequencing Using Conventional, Solexa(Tm), Solid(Tm) Or 454(Tm)-Type Dna Sequencing Platforms To Study Dna Methylation.en_HK
dc.relation.isreferencedbyWO 2012027572 (A2) 2012-03-01en_HK
dc.relation.isreferencedbyWO 2012027572 (A3) 2012-06-07en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License For Public Patent Documents-
dc.titleMethods And Compositions For High-Throughput Bisulphite Dna-Sequencing And Utilitiesen_HK
dc.typePatenten_US
dc.identifier.emailLok, Si:silok@hkucc.hku.hken_US
dc.description.naturelink_to_OA_fulltexten_US
dc.contributor.inventorLok, Sien_US
patents.identifier.applicationUS 12/192393en_HK
patents.description.countryUnited States of Americaen_HK
patents.date.publication2009-02-19en_HK
dc.relation.referencesUS 2009148842 (A1) 2009-06-11en_HK
dc.relation.referencesWO 2005090607 (A1) 2005-09-29en_HK
patents.identifier.hkutechidGRC-2007-00288en_US
patents.date.application2008-08-15en_HK
patents.date.priority2008-08-15 US 12/192393en_HK
patents.date.priority2007-08-15 US /935472Pen_HK
patents.date.priority2007-09-05 US /935867Pen_HK
patents.description.ccUSen_HK
patents.identifier.publicationUS 20090047680en_HK
patents.relation.familyCN 101802223 (A) 2010-08-11en_HK
patents.relation.familyEP 2188389 (A1) 2010-05-26en_HK
patents.relation.familyJP 2010535513 (A) 2010-11-25en_HK
patents.relation.familyUS 2009047680 (A1) 2009-02-19en_HK
patents.relation.familyWO 2009024019 (A1) 2009-02-26en_HK
patents.description.kindA1en_HK
patents.typePatent_publisheden_HK

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