File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Up-regulation of microRNAs, miR21 and miR23a in human liver cancer cells treated with Coptidis rhizoma aqueous extract
  • Basic View
  • Metadata View
  • XML View
TitleUp-regulation of microRNAs, miR21 and miR23a in human liver cancer cells treated with Coptidis rhizoma aqueous extract
 
AuthorsZhu, M1
Wang, N1
Tsao, SW2
Yuen, MF2
Feng, Y1
Wan, TSK2
Man, K2
Feng, Y
 
KeywordsCoptidis rhizoma aqueous extract
Hepatocellular carcinoma
MicroRNA
miR-21
miR-23a
 
Issue Date2011
 
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com
 
CitationExperimental And Therapeutic Medicine, 2011, v. 2 n. 1, p. 27-32 [How to Cite?]
DOI: http://dx.doi.org/10.3892/etm.2010.164
 
AbstractCoptidis rhizoma (CR; Huanglian in Chinese) has been used for the treatment of cancer in Chinese medicine, and recent studies have supported its use in cancer therapy. MicroRNAs (miRNAs) play an important role in the pathophysiology of human cancers. We examined alterations in the miRNA profile of hepatocellular carcinoma (HCC) cells after treatment with coptidis rhizoma aqueous extract (CRAE). An on-chip microarray method was used to detect alterations in the expression profile of miRNAs in human HCCMHCC97-Lcells after exposure to 175 μg/ml CRAE. Altered expression of several miRNAs was detected in the MHCC97-Lcells after treatment with 175 μg/ml CRAE. The microarray results were validated by quantitative real-time PCR(qRT-PCR). Consistent results were obtained; qRT-PCR confirmed that both miR-21 and miR-23a were significantly up-regulated. TargetScan and PicTar microRNA databases were used to predict the possible target genes of the altered miRNAs. The results showed that the altered miRNAs after CRAE treatment may serve as markers for the therapy of liver cancer. To the best of our knowledge, this is the first report on the up-regulation of miRNAs, miR21 and miR23a in human liver cancer cells treated with CRAE. Our results suggest that CRAE targets miR-21 and miR-23a in liver cancer cells supporting the potential application of CRAE in the treatment of HCC.
 
ISSN1792-0981
2012 Impact Factor: 0.344
 
DOIhttp://dx.doi.org/10.3892/etm.2010.164
 
ISI Accession Number IDWOS:000286365000004
Funding AgencyGrant Number
research council of the University of Hong Kong200907176140
Research Grants Council (RGC) of Hong Kong SAR, China764708M
Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine20005274
Hong Kong Government20740314
Funding Information:

The present study was financially supported by grants from the research council of the University of Hong Kong (project code, 200907176140), the Research Grants Council (RGC) of Hong Kong SAR, China (project code, 764708M), the Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine (project code, 20005274) and the Hong Kong Government-Matching Grant Scheme (4th Phase, project code, 20740314). The cell line MHCC97-L was a kind gift from the Liver Cancer Institute Fudan University, Shanghai, China. The authors would like to express thanks to Dr Ka-Yu Siu, Ms. Cindy Lee, Mr. Keith Wong and Mr. Freddy Tsang for the technical support.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorZhu, M
 
dc.contributor.authorWang, N
 
dc.contributor.authorTsao, SW
 
dc.contributor.authorYuen, MF
 
dc.contributor.authorFeng, Y
 
dc.contributor.authorWan, TSK
 
dc.contributor.authorMan, K
 
dc.contributor.authorFeng, Y
 
dc.date.accessioned2011-08-26T14:41:21Z
 
dc.date.available2011-08-26T14:41:21Z
 
dc.date.issued2011
 
dc.description.abstractCoptidis rhizoma (CR; Huanglian in Chinese) has been used for the treatment of cancer in Chinese medicine, and recent studies have supported its use in cancer therapy. MicroRNAs (miRNAs) play an important role in the pathophysiology of human cancers. We examined alterations in the miRNA profile of hepatocellular carcinoma (HCC) cells after treatment with coptidis rhizoma aqueous extract (CRAE). An on-chip microarray method was used to detect alterations in the expression profile of miRNAs in human HCCMHCC97-Lcells after exposure to 175 μg/ml CRAE. Altered expression of several miRNAs was detected in the MHCC97-Lcells after treatment with 175 μg/ml CRAE. The microarray results were validated by quantitative real-time PCR(qRT-PCR). Consistent results were obtained; qRT-PCR confirmed that both miR-21 and miR-23a were significantly up-regulated. TargetScan and PicTar microRNA databases were used to predict the possible target genes of the altered miRNAs. The results showed that the altered miRNAs after CRAE treatment may serve as markers for the therapy of liver cancer. To the best of our knowledge, this is the first report on the up-regulation of miRNAs, miR21 and miR23a in human liver cancer cells treated with CRAE. Our results suggest that CRAE targets miR-21 and miR-23a in liver cancer cells supporting the potential application of CRAE in the treatment of HCC.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationExperimental And Therapeutic Medicine, 2011, v. 2 n. 1, p. 27-32 [How to Cite?]
DOI: http://dx.doi.org/10.3892/etm.2010.164
 
dc.identifier.doihttp://dx.doi.org/10.3892/etm.2010.164
 
dc.identifier.epage32
 
dc.identifier.hkuros191223
 
dc.identifier.isiWOS:000286365000004
Funding AgencyGrant Number
research council of the University of Hong Kong200907176140
Research Grants Council (RGC) of Hong Kong SAR, China764708M
Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine20005274
Hong Kong Government20740314
Funding Information:

The present study was financially supported by grants from the research council of the University of Hong Kong (project code, 200907176140), the Research Grants Council (RGC) of Hong Kong SAR, China (project code, 764708M), the Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine (project code, 20005274) and the Hong Kong Government-Matching Grant Scheme (4th Phase, project code, 20740314). The cell line MHCC97-L was a kind gift from the Liver Cancer Institute Fudan University, Shanghai, China. The authors would like to express thanks to Dr Ka-Yu Siu, Ms. Cindy Lee, Mr. Keith Wong and Mr. Freddy Tsang for the technical support.

 
dc.identifier.issn1792-0981
2012 Impact Factor: 0.344
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.scopuseid_2-s2.0-78751622851
 
dc.identifier.spage27
 
dc.identifier.urihttp://hdl.handle.net/10722/138139
 
dc.identifier.volume2
 
dc.languageeng
 
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com
 
dc.publisher.placeGreece
 
dc.relation.ispartofExperimental and Therapeutic Medicine
 
dc.relation.referencesReferences in Scopus
 
dc.subjectCoptidis rhizoma aqueous extract
 
dc.subjectHepatocellular carcinoma
 
dc.subjectMicroRNA
 
dc.subjectmiR-21
 
dc.subjectmiR-23a
 
dc.titleUp-regulation of microRNAs, miR21 and miR23a in human liver cancer cells treated with Coptidis rhizoma aqueous extract
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Zhu, M</contributor.author>
<contributor.author>Wang, N</contributor.author>
<contributor.author>Tsao, SW</contributor.author>
<contributor.author>Yuen, MF</contributor.author>
<contributor.author>Feng, Y</contributor.author>
<contributor.author>Wan, TSK</contributor.author>
<contributor.author>Man, K</contributor.author>
<contributor.author>Feng, Y</contributor.author>
<date.accessioned>2011-08-26T14:41:21Z</date.accessioned>
<date.available>2011-08-26T14:41:21Z</date.available>
<date.issued>2011</date.issued>
<identifier.citation>Experimental And Therapeutic Medicine, 2011, v. 2 n. 1, p. 27-32</identifier.citation>
<identifier.issn>1792-0981</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/138139</identifier.uri>
<description.abstract>Coptidis rhizoma (CR; Huanglian in Chinese) has been used for the treatment of cancer in Chinese medicine, and recent studies have supported its use in cancer therapy. MicroRNAs (miRNAs) play an important role in the pathophysiology of human cancers. We examined alterations in the miRNA profile of hepatocellular carcinoma (HCC) cells after treatment with coptidis rhizoma aqueous extract (CRAE). An on-chip microarray method was used to detect alterations in the expression profile of miRNAs in human HCCMHCC97-Lcells after exposure to 175 &#956;g/ml CRAE. Altered expression of several miRNAs was detected in the MHCC97-Lcells after treatment with 175 &#956;g/ml CRAE. The microarray results were validated by quantitative real-time PCR(qRT-PCR). Consistent results were obtained; qRT-PCR confirmed that both miR-21 and miR-23a were significantly up-regulated. TargetScan and PicTar microRNA databases were used to predict the possible target genes of the altered miRNAs. The results showed that the altered miRNAs after CRAE treatment may serve as markers for the therapy of liver cancer. To the best of our knowledge, this is the first report on the up-regulation of miRNAs, miR21 and miR23a in human liver cancer cells treated with CRAE. Our results suggest that CRAE targets miR-21 and miR-23a in liver cancer cells supporting the potential application of CRAE in the treatment of HCC.</description.abstract>
<language>eng</language>
<publisher>Spandidos Publications. The Journal&apos;s web site is located at http://www.spandidos-publications.com </publisher>
<relation.ispartof>Experimental and Therapeutic Medicine</relation.ispartof>
<subject>Coptidis rhizoma aqueous extract</subject>
<subject>Hepatocellular carcinoma</subject>
<subject>MicroRNA</subject>
<subject>miR-21</subject>
<subject>miR-23a</subject>
<title>Up-regulation of microRNAs, miR21 and miR23a in human liver cancer cells treated with Coptidis rhizoma aqueous extract</title>
<type>Article</type>
<identifier.openurl>http://library.hku.hk:4550/resserv?sid=HKU:IR&amp;issn=1792-0981&amp;volume=2&amp;issue=1&amp;spage=27&amp;epage=32&amp;date=2011&amp;atitle=Up-regulation+of+microRNAs,+miR21+and+miR23a,+in+human+liver+cancer+cells+treated+with+Coptidis+rhizome+aqueous+extract</identifier.openurl>
<description.nature>link_to_OA_fulltext</description.nature>
<identifier.doi>10.3892/etm.2010.164</identifier.doi>
<identifier.scopus>eid_2-s2.0-78751622851</identifier.scopus>
<identifier.hkuros>191223</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-78751622851&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>2</identifier.volume>
<identifier.issue>1</identifier.issue>
<identifier.spage>27</identifier.spage>
<identifier.epage>32</identifier.epage>
<identifier.isi>WOS:000286365000004</identifier.isi>
<publisher.place>Greece</publisher.place>
<bitstream.url>http://hub.hku.hk/bitstream/10722/138139/1/re01.htm</bitstream.url>
</item>
Author Affiliations
  1. School of Chinese Medicine
  2. The University of Hong Kong
  3. Sun Yat-Sen University