Article: Up-regulation of microRNAs, miR21 and miR23a in human liver cancer cells treated with Coptidis rhizoma aqueous extract
File Download
Links for fulltext
(May Require Subscription)
(May Require Subscription)
- Publisher Website: 10.3892/etm.2010.164
- Scopus: eid_2-s2.0-78751622851
- Find via
Supplementary
-
Citations:
| Title | Up-regulation of microRNAs, miR21 and miR23a in human liver cancer cells treated with Coptidis rhizoma aqueous extract | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Authors | Zhu, M1 Wang, N1 Tsao, SW2 Yuen, MF2 Feng, Y1 Wan, TSK2 Man, K2 Feng, Y | ||||||||||
| Keywords | Coptidis rhizoma aqueous extract Hepatocellular carcinoma MicroRNA miR-21 miR-23a | ||||||||||
| Issue Date | 2011 | ||||||||||
| Publisher | Spandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com | ||||||||||
| Citation | Experimental And Therapeutic Medicine, 2011, v. 2 n. 1, p. 27-32 [How to Cite?] DOI: http://dx.doi.org/10.3892/etm.2010.164 | ||||||||||
| Abstract | Coptidis rhizoma (CR; Huanglian in Chinese) has been used for the treatment of cancer in Chinese medicine, and recent studies have supported its use in cancer therapy. MicroRNAs (miRNAs) play an important role in the pathophysiology of human cancers. We examined alterations in the miRNA profile of hepatocellular carcinoma (HCC) cells after treatment with coptidis rhizoma aqueous extract (CRAE). An on-chip microarray method was used to detect alterations in the expression profile of miRNAs in human HCCMHCC97-Lcells after exposure to 175 μg/ml CRAE. Altered expression of several miRNAs was detected in the MHCC97-Lcells after treatment with 175 μg/ml CRAE. The microarray results were validated by quantitative real-time PCR(qRT-PCR). Consistent results were obtained; qRT-PCR confirmed that both miR-21 and miR-23a were significantly up-regulated. TargetScan and PicTar microRNA databases were used to predict the possible target genes of the altered miRNAs. The results showed that the altered miRNAs after CRAE treatment may serve as markers for the therapy of liver cancer. To the best of our knowledge, this is the first report on the up-regulation of miRNAs, miR21 and miR23a in human liver cancer cells treated with CRAE. Our results suggest that CRAE targets miR-21 and miR-23a in liver cancer cells supporting the potential application of CRAE in the treatment of HCC. | ||||||||||
| ISSN | 1792-0981 2011 Impact Factor: 0.206 | ||||||||||
| DOI | http://dx.doi.org/10.3892/etm.2010.164 | ||||||||||
| ISI Accession Number ID | WOS:000286365000004
Funding Information: The present study was financially supported by grants from the research council of the University of Hong Kong (project code, 200907176140), the Research Grants Council (RGC) of Hong Kong SAR, China (project code, 764708M), the Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine (project code, 20005274) and the Hong Kong Government-Matching Grant Scheme (4th Phase, project code, 20740314). The cell line MHCC97-L was a kind gift from the Liver Cancer Institute Fudan University, Shanghai, China. The authors would like to express thanks to Dr Ka-Yu Siu, Ms. Cindy Lee, Mr. Keith Wong and Mr. Freddy Tsang for the technical support. | ||||||||||
| References | References in Scopus | ||||||||||
| Grants | The role of autophagy and mitochondrial apoptosis in berberine induced hepatocellular caricinoma cell death and its underlying mechanism |
| dc.contributor.author | Zhu, M | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| dc.contributor.author | Wang, N | ||||||||||
| dc.contributor.author | Tsao, SW | ||||||||||
| dc.contributor.author | Yuen, MF | ||||||||||
| dc.contributor.author | Feng, Y | ||||||||||
| dc.contributor.author | Wan, TSK | ||||||||||
| dc.contributor.author | Man, K | ||||||||||
| dc.contributor.author | Feng, Y | ||||||||||
| dc.date.accessioned | 2011-08-26T14:41:21Z | ||||||||||
| dc.date.available | 2011-08-26T14:41:21Z | ||||||||||
| dc.date.issued | 2011 | ||||||||||
| dc.description.abstract | Coptidis rhizoma (CR; Huanglian in Chinese) has been used for the treatment of cancer in Chinese medicine, and recent studies have supported its use in cancer therapy. MicroRNAs (miRNAs) play an important role in the pathophysiology of human cancers. We examined alterations in the miRNA profile of hepatocellular carcinoma (HCC) cells after treatment with coptidis rhizoma aqueous extract (CRAE). An on-chip microarray method was used to detect alterations in the expression profile of miRNAs in human HCCMHCC97-Lcells after exposure to 175 μg/ml CRAE. Altered expression of several miRNAs was detected in the MHCC97-Lcells after treatment with 175 μg/ml CRAE. The microarray results were validated by quantitative real-time PCR(qRT-PCR). Consistent results were obtained; qRT-PCR confirmed that both miR-21 and miR-23a were significantly up-regulated. TargetScan and PicTar microRNA databases were used to predict the possible target genes of the altered miRNAs. The results showed that the altered miRNAs after CRAE treatment may serve as markers for the therapy of liver cancer. To the best of our knowledge, this is the first report on the up-regulation of miRNAs, miR21 and miR23a in human liver cancer cells treated with CRAE. Our results suggest that CRAE targets miR-21 and miR-23a in liver cancer cells supporting the potential application of CRAE in the treatment of HCC. | ||||||||||
| dc.description.grant | The role of autophagy and mitochondrial apoptosis in berberine induced hepatocellular caricinoma cell death and its underlying mechanism | ||||||||||
| dc.description.grantcode | 101411 | ||||||||||
| dc.description.nature | link_to_OA_fulltext | ||||||||||
| dc.identifier.citation | Experimental And Therapeutic Medicine, 2011, v. 2 n. 1, p. 27-32 [How to Cite?] DOI: http://dx.doi.org/10.3892/etm.2010.164 | ||||||||||
| dc.identifier.doi | http://dx.doi.org/10.3892/etm.2010.164 | ||||||||||
| dc.identifier.epage | 32 | ||||||||||
| dc.identifier.hkuros | 191223 | ||||||||||
| dc.identifier.isi | WOS:000286365000004
Funding Information: The present study was financially supported by grants from the research council of the University of Hong Kong (project code, 200907176140), the Research Grants Council (RGC) of Hong Kong SAR, China (project code, 764708M), the Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine (project code, 20005274) and the Hong Kong Government-Matching Grant Scheme (4th Phase, project code, 20740314). The cell line MHCC97-L was a kind gift from the Liver Cancer Institute Fudan University, Shanghai, China. The authors would like to express thanks to Dr Ka-Yu Siu, Ms. Cindy Lee, Mr. Keith Wong and Mr. Freddy Tsang for the technical support. | ||||||||||
| dc.identifier.issn | 1792-0981 2011 Impact Factor: 0.206 | ||||||||||
| dc.identifier.issue | 1 | ||||||||||
| dc.identifier.openurl | | ||||||||||
| dc.identifier.scopus | eid_2-s2.0-78751622851 | ||||||||||
| dc.identifier.spage | 27 | ||||||||||
| dc.identifier.uri | http://hdl.handle.net/10722/138139 | ||||||||||
| dc.identifier.volume | 2 | ||||||||||
| dc.language | eng | ||||||||||
| dc.publisher | Spandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com | ||||||||||
| dc.publisher.place | Greece | ||||||||||
| dc.relation.ispartof | Experimental and Therapeutic Medicine | ||||||||||
| dc.relation.references | References in Scopus | ||||||||||
| dc.subject | Coptidis rhizoma aqueous extract | ||||||||||
| dc.subject | Hepatocellular carcinoma | ||||||||||
| dc.subject | MicroRNA | ||||||||||
| dc.subject | miR-21 | ||||||||||
| dc.subject | miR-23a | ||||||||||
| dc.title | Up-regulation of microRNAs, miR21 and miR23a in human liver cancer cells treated with Coptidis rhizoma aqueous extract | ||||||||||
| dc.type | Article |
Author Affiliations
- School of Chinese Medicine
- The University of Hong Kong
- Sun Yat-Sen University