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Article: The role of Pea3 group transcription factors in esophageal squamous cell carcinoma
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TitleThe role of Pea3 group transcription factors in esophageal squamous cell carcinoma
 
AuthorsYuen, HF2
McCrudden, CM2
Chan, KK2
Chan, YP1
Wong, MLY1
Chan, KYK1
Khoo, US1
Law, S1
Srivastava, G1
Lappin, TR2
Chan, KW1
ElTanani, M2 1
 
KeywordsApoptosis
Cancer growth
Cancer inhibition
Cancer invasion
Cancer resistance
 
Issue Date2011
 
PublisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
 
CitationAmerican Journal Of Pathology, 2011, v. 179 n. 2, p. 992-1003 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ajpath.2011.04.004
 
AbstractThe transcription factors Pea3, Erm, and Er81 can promote cancer initiation and progression in various types of solid tumors. However, their role in esophageal squamous cell carcinoma (ESCC) has not been elucidated. In this study, we found that the expression levels of Pea3 and Erm, but not that of Er81, were significantly higher in ESCC compared with nontumor esophageal epithelium. A high level of Pea3 expression was significantly correlated with a shorter overall survival in a cohort of 81 patients with ESCC and the subgroup with N1 stage tumor (Wilcoxon-Gehan test, P = 0.016 and P = 0.001, respectively). Pea3 was overexpressed in seven ESCC cell lines compared with two immortalized esophageal cell lines. Pea3 knockdown reduced cell proliferation and suppressed nonadherent growth, migration, and invasion in ESCC cells in vitro. In addition, Pea3 knockdown in ESCC cells resulted in a down-regulation of phospho-Akt and matrix metalloproteinase 13, whereas a significant positive correlation in the expression levels was observed between Pea3 and phospho-Akt (r = 0.281, P < 0.013) and between Pea3 and matrix metalloproteinase 13 in the human specimens (r = 0.462, P < 0.001). Moreover, Pea3 modulated the sensitivity of EC109 cells to doxorubicin, probably via reduced activity of the phosphatidylinositol 3-kinaseAktmammalian target of Rapamycin complex 1 pathway on Pea3 knockdown. In conclusion, our results suggest that Pea3 plays an important role in the progression of ESCC. © 2011 American Society for Investigative Pathology.
 
ISSN0002-9440
2013 Impact Factor: 4.602
 
DOIhttp://dx.doi.org/10.1016/j.ajpath.2011.04.004
 
PubMed Central IDPMC3157177
 
ISI Accession Number IDWOS:000298307200043
Funding AgencyGrant Number
Cancer Research UK
Funding Information:

Supported by a postdoctoral fellowship from Cancer Research UK (H.-F.Y.).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYuen, HF
 
dc.contributor.authorMcCrudden, CM
 
dc.contributor.authorChan, KK
 
dc.contributor.authorChan, YP
 
dc.contributor.authorWong, MLY
 
dc.contributor.authorChan, KYK
 
dc.contributor.authorKhoo, US
 
dc.contributor.authorLaw, S
 
dc.contributor.authorSrivastava, G
 
dc.contributor.authorLappin, TR
 
dc.contributor.authorChan, KW
 
dc.contributor.authorElTanani, M
 
dc.date.accessioned2011-08-26T14:29:51Z
 
dc.date.available2011-08-26T14:29:51Z
 
dc.date.issued2011
 
dc.description.abstractThe transcription factors Pea3, Erm, and Er81 can promote cancer initiation and progression in various types of solid tumors. However, their role in esophageal squamous cell carcinoma (ESCC) has not been elucidated. In this study, we found that the expression levels of Pea3 and Erm, but not that of Er81, were significantly higher in ESCC compared with nontumor esophageal epithelium. A high level of Pea3 expression was significantly correlated with a shorter overall survival in a cohort of 81 patients with ESCC and the subgroup with N1 stage tumor (Wilcoxon-Gehan test, P = 0.016 and P = 0.001, respectively). Pea3 was overexpressed in seven ESCC cell lines compared with two immortalized esophageal cell lines. Pea3 knockdown reduced cell proliferation and suppressed nonadherent growth, migration, and invasion in ESCC cells in vitro. In addition, Pea3 knockdown in ESCC cells resulted in a down-regulation of phospho-Akt and matrix metalloproteinase 13, whereas a significant positive correlation in the expression levels was observed between Pea3 and phospho-Akt (r = 0.281, P < 0.013) and between Pea3 and matrix metalloproteinase 13 in the human specimens (r = 0.462, P < 0.001). Moreover, Pea3 modulated the sensitivity of EC109 cells to doxorubicin, probably via reduced activity of the phosphatidylinositol 3-kinaseAktmammalian target of Rapamycin complex 1 pathway on Pea3 knockdown. In conclusion, our results suggest that Pea3 plays an important role in the progression of ESCC. © 2011 American Society for Investigative Pathology.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationAmerican Journal Of Pathology, 2011, v. 179 n. 2, p. 992-1003 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ajpath.2011.04.004
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.ajpath.2011.04.004
 
dc.identifier.eissn1525-2191
 
dc.identifier.epage1003
 
dc.identifier.hkuros191693
 
dc.identifier.isiWOS:000298307200043
Funding AgencyGrant Number
Cancer Research UK
Funding Information:

Supported by a postdoctoral fellowship from Cancer Research UK (H.-F.Y.).

 
dc.identifier.issn0002-9440
2013 Impact Factor: 4.602
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC3157177
 
dc.identifier.pmid21689625
 
dc.identifier.scopuseid_2-s2.0-80052485012
 
dc.identifier.spage992
 
dc.identifier.urihttp://hdl.handle.net/10722/137634
 
dc.identifier.volume179
 
dc.languageeng
 
dc.publisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
 
dc.publisher.placeUnited States
 
dc.relation.ispartofAmerican Journal of Pathology
 
dc.relation.referencesReferences in Scopus
 
dc.subjectApoptosis
 
dc.subjectCancer growth
 
dc.subjectCancer inhibition
 
dc.subjectCancer invasion
 
dc.subjectCancer resistance
 
dc.titleThe role of Pea3 group transcription factors in esophageal squamous cell carcinoma
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Queen's University Belfast