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Article: F-actin reorganization and inactivation of Rho signaling pathway involved in the inhibitory effect of Coptidis Rhizoma on hepatoma cell migration
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TitleF-actin reorganization and inactivation of Rho signaling pathway involved in the inhibitory effect of Coptidis Rhizoma on hepatoma cell migration
 
AuthorsWang, N2
Feng, Y2
Lau, EPW2
Tsang, C2
Ching, Y2
Man, K2
Tong, Y2
Nagamatsu, T1
Su, W3
Tsao, S2
 
Keywordsantimetastasis
berberine
Coptidis Rhizoma
F-actin
hepatocellular carcinoma
Ro/ROCK signaling
 
Issue Date2010
 
PublisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201510
 
CitationIntegrative Cancer Therapies, 2010, v. 9 n. 4, p. 354-364 [How to Cite?]
DOI: http://dx.doi.org/10.1177/1534735410379121
 
AbstractHypothesis. Hepatocellular carcinoma (HCC) is one of the most malignant human tumors and one of the risk factors is its highly metastatic property. Coptidis Rhizoma aqueous extract (CRAE) is able to suppress the migration and invasion of HCC cells, MHCC97-L, and F-actin reorganization and Rho signaling inhibition is involved. Main methods. CRAE was prepared and analyzed by high-performance liquid chromatography combined with mass spectrometry. The cytotoxicity and antimigration action of CRAE on MHCC97-L cells were evaluated; Immunofluorescence and immunoblotting were used to investigate the proposed mechanism of CRAE action. Key findings. Chemical analysis reveals that the active components in CRAE are berberine and berberine-like alkaloids. CRAE exhibits a significant inhibitory effect on MHCC97-L cell migration as indicated by wound-healing and invasion-chamber assays. No significant alteration of matrix metalloproteinases and urokinase-type plasminogen activator (uPA) expression were observed in MHCC97-L cells exposed to CRAE. Reduction of F-actin polymerization and damage to cytoskeleton network in MHCC97-L cells were observed after CRAE treatment. Furthermore, it was found that CRAE significantly downregulated the Rho/ROCK signaling pathway. Significance. These results indicate that CRAE may act as a Rho/ROCK signaling inhibitor to suppress MHCC97-L cell migration in vitro and suggested that total alkaloids in Coptidis Rhizoma may be a potential agent for suppressing liver cancer invasion. © 2010 SAGE Publications.
 
ISSN1534-7354
2012 Impact Factor: 2.354
2012 SCImago Journal Rankings: 0.584
 
DOIhttp://dx.doi.org/10.1177/1534735410379121
 
ISI Accession Number IDWOS:000284588500009
Funding AgencyGrant Number
University of Hong Kong200811159197
200907176140
University Grant Committee (UGC) of Hong Kong SAR of China764708M
Pong Ding Yueng Endowment Fund for Education & Research in Chinese-Western Medicine20005274
Government-Matching Grant Scheme20740314
Funding Information:

This study was financially supported by grants from the research council of the University of Hong Kong (Project Codes: 200811159197 and 200907176140), The University Grant Committee (UGC) of Hong Kong SAR of China (Project Code: 764708M), Pong Ding Yueng Endowment Fund for Education & Research in Chinese-Western Medicine (Project Code: 20005274), and Government-Matching Grant Scheme (4th Phase, Project Code: 20740314).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWang, N
 
dc.contributor.authorFeng, Y
 
dc.contributor.authorLau, EPW
 
dc.contributor.authorTsang, C
 
dc.contributor.authorChing, Y
 
dc.contributor.authorMan, K
 
dc.contributor.authorTong, Y
 
dc.contributor.authorNagamatsu, T
 
dc.contributor.authorSu, W
 
dc.contributor.authorTsao, S
 
dc.date.accessioned2010-10-31T13:34:42Z
 
dc.date.available2010-10-31T13:34:42Z
 
dc.date.issued2010
 
dc.description.abstractHypothesis. Hepatocellular carcinoma (HCC) is one of the most malignant human tumors and one of the risk factors is its highly metastatic property. Coptidis Rhizoma aqueous extract (CRAE) is able to suppress the migration and invasion of HCC cells, MHCC97-L, and F-actin reorganization and Rho signaling inhibition is involved. Main methods. CRAE was prepared and analyzed by high-performance liquid chromatography combined with mass spectrometry. The cytotoxicity and antimigration action of CRAE on MHCC97-L cells were evaluated; Immunofluorescence and immunoblotting were used to investigate the proposed mechanism of CRAE action. Key findings. Chemical analysis reveals that the active components in CRAE are berberine and berberine-like alkaloids. CRAE exhibits a significant inhibitory effect on MHCC97-L cell migration as indicated by wound-healing and invasion-chamber assays. No significant alteration of matrix metalloproteinases and urokinase-type plasminogen activator (uPA) expression were observed in MHCC97-L cells exposed to CRAE. Reduction of F-actin polymerization and damage to cytoskeleton network in MHCC97-L cells were observed after CRAE treatment. Furthermore, it was found that CRAE significantly downregulated the Rho/ROCK signaling pathway. Significance. These results indicate that CRAE may act as a Rho/ROCK signaling inhibitor to suppress MHCC97-L cell migration in vitro and suggested that total alkaloids in Coptidis Rhizoma may be a potential agent for suppressing liver cancer invasion. © 2010 SAGE Publications.
 
dc.description.naturepostprint
 
dc.identifier.citationIntegrative Cancer Therapies, 2010, v. 9 n. 4, p. 354-364 [How to Cite?]
DOI: http://dx.doi.org/10.1177/1534735410379121
 
dc.identifier.doihttp://dx.doi.org/10.1177/1534735410379121
 
dc.identifier.epage364
 
dc.identifier.hkuros176203
 
dc.identifier.hkuros191224
 
dc.identifier.isiWOS:000284588500009
Funding AgencyGrant Number
University of Hong Kong200811159197
200907176140
University Grant Committee (UGC) of Hong Kong SAR of China764708M
Pong Ding Yueng Endowment Fund for Education & Research in Chinese-Western Medicine20005274
Government-Matching Grant Scheme20740314
Funding Information:

This study was financially supported by grants from the research council of the University of Hong Kong (Project Codes: 200811159197 and 200907176140), The University Grant Committee (UGC) of Hong Kong SAR of China (Project Code: 764708M), Pong Ding Yueng Endowment Fund for Education & Research in Chinese-Western Medicine (Project Code: 20005274), and Government-Matching Grant Scheme (4th Phase, Project Code: 20740314).

 
dc.identifier.issn1534-7354
2012 Impact Factor: 2.354
2012 SCImago Journal Rankings: 0.584
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmid21106616
 
dc.identifier.scopuseid_2-s2.0-78649654950
 
dc.identifier.spage354
 
dc.identifier.urihttp://hdl.handle.net/10722/127596
 
dc.identifier.volume9
 
dc.languageeng
 
dc.publisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201510
 
dc.publisher.placeUnited States
 
dc.relation.ispartofIntegrative Cancer Therapies
 
dc.relation.referencesReferences in Scopus
 
dc.rightsIntegrative Cancer Therapies. Copyright © Sage Publications, Inc..
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subjectantimetastasis
 
dc.subjectberberine
 
dc.subjectCoptidis Rhizoma
 
dc.subjectF-actin
 
dc.subjecthepatocellular carcinoma
 
dc.subjectRo/ROCK signaling
 
dc.titleF-actin reorganization and inactivation of Rho signaling pathway involved in the inhibitory effect of Coptidis Rhizoma on hepatoma cell migration
 
dc.typeArticle
 
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Author Affiliations
  1. Meijo University
  2. The University of Hong Kong
  3. Sun Yat-Sen University