File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: F-actin reorganization and inactivation of Rho signaling pathway involved in the inhibitory effect of Coptidis Rhizoma on hepatoma cell migration

TitleF-actin reorganization and inactivation of Rho signaling pathway involved in the inhibitory effect of Coptidis Rhizoma on hepatoma cell migration
Authors
Keywordsantimetastasis
berberine
Coptidis Rhizoma
F-actin
hepatocellular carcinoma
Ro/ROCK signaling
Issue Date2010
PublisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201510
Citation
Integrative Cancer Therapies, 2010, v. 9 n. 4, p. 354-364 How to Cite?
Abstract
Hypothesis. Hepatocellular carcinoma (HCC) is one of the most malignant human tumors and one of the risk factors is its highly metastatic property. Coptidis Rhizoma aqueous extract (CRAE) is able to suppress the migration and invasion of HCC cells, MHCC97-L, and F-actin reorganization and Rho signaling inhibition is involved. Main methods. CRAE was prepared and analyzed by high-performance liquid chromatography combined with mass spectrometry. The cytotoxicity and antimigration action of CRAE on MHCC97-L cells were evaluated; Immunofluorescence and immunoblotting were used to investigate the proposed mechanism of CRAE action. Key findings. Chemical analysis reveals that the active components in CRAE are berberine and berberine-like alkaloids. CRAE exhibits a significant inhibitory effect on MHCC97-L cell migration as indicated by wound-healing and invasion-chamber assays. No significant alteration of matrix metalloproteinases and urokinase-type plasminogen activator (uPA) expression were observed in MHCC97-L cells exposed to CRAE. Reduction of F-actin polymerization and damage to cytoskeleton network in MHCC97-L cells were observed after CRAE treatment. Furthermore, it was found that CRAE significantly downregulated the Rho/ROCK signaling pathway. Significance. These results indicate that CRAE may act as a Rho/ROCK signaling inhibitor to suppress MHCC97-L cell migration in vitro and suggested that total alkaloids in Coptidis Rhizoma may be a potential agent for suppressing liver cancer invasion. © 2010 SAGE Publications.
Persistent Identifierhttp://hdl.handle.net/10722/127596
ISSN
2013 Impact Factor: 2.014
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong200811159197
200907176140
University Grant Committee (UGC) of Hong Kong SAR of China764708M
Pong Ding Yueng Endowment Fund for Education & Research in Chinese-Western Medicine20005274
Government-Matching Grant Scheme20740314
Funding Information:

This study was financially supported by grants from the research council of the University of Hong Kong (Project Codes: 200811159197 and 200907176140), The University Grant Committee (UGC) of Hong Kong SAR of China (Project Code: 764708M), Pong Ding Yueng Endowment Fund for Education & Research in Chinese-Western Medicine (Project Code: 20005274), and Government-Matching Grant Scheme (4th Phase, Project Code: 20740314).

References

 

DC FieldValueLanguage
dc.contributor.authorWang, Nen_HK
dc.contributor.authorFeng, Yen_HK
dc.contributor.authorLau, EPWen_HK
dc.contributor.authorTsang, Cen_HK
dc.contributor.authorChing, Yen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorTong, Yen_HK
dc.contributor.authorNagamatsu, Ten_HK
dc.contributor.authorSu, Wen_HK
dc.contributor.authorTsao, Sen_HK
dc.date.accessioned2010-10-31T13:34:42Z-
dc.date.available2010-10-31T13:34:42Z-
dc.date.issued2010en_HK
dc.identifier.citationIntegrative Cancer Therapies, 2010, v. 9 n. 4, p. 354-364en_HK
dc.identifier.issn1534-7354en_HK
dc.identifier.urihttp://hdl.handle.net/10722/127596-
dc.description.abstractHypothesis. Hepatocellular carcinoma (HCC) is one of the most malignant human tumors and one of the risk factors is its highly metastatic property. Coptidis Rhizoma aqueous extract (CRAE) is able to suppress the migration and invasion of HCC cells, MHCC97-L, and F-actin reorganization and Rho signaling inhibition is involved. Main methods. CRAE was prepared and analyzed by high-performance liquid chromatography combined with mass spectrometry. The cytotoxicity and antimigration action of CRAE on MHCC97-L cells were evaluated; Immunofluorescence and immunoblotting were used to investigate the proposed mechanism of CRAE action. Key findings. Chemical analysis reveals that the active components in CRAE are berberine and berberine-like alkaloids. CRAE exhibits a significant inhibitory effect on MHCC97-L cell migration as indicated by wound-healing and invasion-chamber assays. No significant alteration of matrix metalloproteinases and urokinase-type plasminogen activator (uPA) expression were observed in MHCC97-L cells exposed to CRAE. Reduction of F-actin polymerization and damage to cytoskeleton network in MHCC97-L cells were observed after CRAE treatment. Furthermore, it was found that CRAE significantly downregulated the Rho/ROCK signaling pathway. Significance. These results indicate that CRAE may act as a Rho/ROCK signaling inhibitor to suppress MHCC97-L cell migration in vitro and suggested that total alkaloids in Coptidis Rhizoma may be a potential agent for suppressing liver cancer invasion. © 2010 SAGE Publications.en_HK
dc.languageengen_HK
dc.publisherSage Publications, Inc.. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201510en_HK
dc.relation.ispartofIntegrative Cancer Therapiesen_HK
dc.rightsIntegrative Cancer Therapies. Copyright © Sage Publications, Inc..-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectantimetastasisen_HK
dc.subjectberberineen_HK
dc.subjectCoptidis Rhizomaen_HK
dc.subjectF-actinen_HK
dc.subjecthepatocellular carcinomaen_HK
dc.subjectRo/ROCK signalingen_HK
dc.titleF-actin reorganization and inactivation of Rho signaling pathway involved in the inhibitory effect of Coptidis Rhizoma on hepatoma cell migrationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1534-7354&volume=9&issue=4&spage=354&epage=364&date=2010&atitle=F-actin+reorganization+and+inactivation+of+Rho+signaling+pathway+involved+in+the+inhibitory+effect+of+Coptidis+Rhizoma+on+hepatoma+cell+migrationen_HK
dc.identifier.emailFeng, Y: yfeng@hku.hken_HK
dc.identifier.emailChing, Y: ypching@hku.hken_HK
dc.identifier.emailMan, K: kwanman@hku.hken_HK
dc.identifier.emailTong, Y: tongyao@hku.hken_HK
dc.identifier.emailTsao, S: gswtsao@hku.hken_HK
dc.identifier.authorityFeng, Y=rp00466en_HK
dc.identifier.authorityChing, Y=rp00469en_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityTong, Y=rp00509en_HK
dc.identifier.authorityTsao, S=rp00399en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1177/1534735410379121en_HK
dc.identifier.pmid21106616en_HK
dc.identifier.scopuseid_2-s2.0-78649654950en_HK
dc.identifier.hkuros176203en_HK
dc.identifier.hkuros191224-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78649654950&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.issue4en_HK
dc.identifier.spage354en_HK
dc.identifier.epage364en_HK
dc.identifier.isiWOS:000284588500009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, N=35072317700en_HK
dc.identifier.scopusauthoridFeng, Y=24467969600en_HK
dc.identifier.scopusauthoridLau, EPW=7103086020en_HK
dc.identifier.scopusauthoridTsang, C=24831236400en_HK
dc.identifier.scopusauthoridChing, Y=7005431277en_HK
dc.identifier.scopusauthoridMan, K=7101754072en_HK
dc.identifier.scopusauthoridTong, Y=9045384000en_HK
dc.identifier.scopusauthoridNagamatsu, T=7006510058en_HK
dc.identifier.scopusauthoridSu, W=7402010268en_HK
dc.identifier.scopusauthoridTsao, S=7102813116en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats