File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1046/j.1529-8817.2003.02190.x
- Scopus: eid_2-s2.0-0038685990
- WOS: WOS:000183070000011
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Identification of a novel cytochrome P450 cDNA, CYP97E1, from the marine diatom Skeletonema costatum bacillariophyceae
Title | Identification of a novel cytochrome P450 cDNA, CYP97E1, from the marine diatom Skeletonema costatum bacillariophyceae |
---|---|
Authors | |
Keywords | Alga Cytochrome P450 Diatom Differential display Rapid amplification of cDNA ends Reverse transcription PCR Skeletonema costatum |
Issue Date | 2003 |
Publisher | Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPY |
Citation | Journal Of Phycology, 2003, v. 39 n. 3, p. 555-560 How to Cite? |
Abstract | Here we report identification of a 2269-base pair full-length cDNA, CYP97E1, encoding a novel cytochrome P450 protein from the marine diatom Skeletonema costatum. The CYP97E1 protein contains 659 amino acids (Mr 74,200) and is the largest P450 isoform described to date. Our BLAST homology search and parsimony analysis showed that CYP97E1 shared high sequence identity (>40%) and genetic relatedness, respectively, with the CYP97B isoforms from different plant species. CYP97E1 was predicted by PSORT (a protein localization site prediction program) to be a cytosolic protein. Northern hybridization analysis indicated that CYP97E1 expression in S. costatum was not significantly affected by 2,4-dichlorophenol, suggesting that CYP97E1 may not be involved in 2,4-dichlorophenol detoxification in this diatom. |
Persistent Identifier | http://hdl.handle.net/10722/92745 |
ISSN | 2023 Impact Factor: 2.8 2023 SCImago Journal Rankings: 0.784 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, S | en_HK |
dc.contributor.author | Wu, RSS | en_HK |
dc.contributor.author | Mok, HOL | en_HK |
dc.contributor.author | Zhang, ZP | en_HK |
dc.contributor.author | Kong, RYC | en_HK |
dc.date.accessioned | 2010-09-17T10:55:55Z | - |
dc.date.available | 2010-09-17T10:55:55Z | - |
dc.date.issued | 2003 | en_HK |
dc.identifier.citation | Journal Of Phycology, 2003, v. 39 n. 3, p. 555-560 | en_HK |
dc.identifier.issn | 0022-3646 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/92745 | - |
dc.description.abstract | Here we report identification of a 2269-base pair full-length cDNA, CYP97E1, encoding a novel cytochrome P450 protein from the marine diatom Skeletonema costatum. The CYP97E1 protein contains 659 amino acids (Mr 74,200) and is the largest P450 isoform described to date. Our BLAST homology search and parsimony analysis showed that CYP97E1 shared high sequence identity (>40%) and genetic relatedness, respectively, with the CYP97B isoforms from different plant species. CYP97E1 was predicted by PSORT (a protein localization site prediction program) to be a cytosolic protein. Northern hybridization analysis indicated that CYP97E1 expression in S. costatum was not significantly affected by 2,4-dichlorophenol, suggesting that CYP97E1 may not be involved in 2,4-dichlorophenol detoxification in this diatom. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JPY | en_HK |
dc.relation.ispartof | Journal of Phycology | en_HK |
dc.subject | Alga | en_HK |
dc.subject | Cytochrome P450 | en_HK |
dc.subject | Diatom | en_HK |
dc.subject | Differential display | en_HK |
dc.subject | Rapid amplification of cDNA ends | en_HK |
dc.subject | Reverse transcription PCR | en_HK |
dc.subject | Skeletonema costatum | en_HK |
dc.title | Identification of a novel cytochrome P450 cDNA, CYP97E1, from the marine diatom Skeletonema costatum bacillariophyceae | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Wu, RSS: rudolfwu@hku.hk | en_HK |
dc.identifier.authority | Wu, RSS=rp01398 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1046/j.1529-8817.2003.02190.x | en_HK |
dc.identifier.scopus | eid_2-s2.0-0038685990 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0038685990&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 39 | en_HK |
dc.identifier.issue | 3 | en_HK |
dc.identifier.spage | 555 | en_HK |
dc.identifier.epage | 560 | en_HK |
dc.identifier.isi | WOS:000183070000011 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Yang, S=7406950684 | en_HK |
dc.identifier.scopusauthorid | Wu, RSS=7402945079 | en_HK |
dc.identifier.scopusauthorid | Mok, HOL=7005842490 | en_HK |
dc.identifier.scopusauthorid | Zhang, ZP=35228251200 | en_HK |
dc.identifier.scopusauthorid | Kong, RYC=7005290687 | en_HK |
dc.identifier.issnl | 0022-3646 | - |