The expression of hGCN5 in burkitt lymphma Daudi cells and effets of TSA rgulating cell antiproliferation and apoptosis

Abstract

Objective: To investigate the effect of TSA (Trichostatin A) regulating hGCN5 (human general control of amino acid synthesis protein 5) in B-NHL cell line Daudi cells, and to study anti-proliferation molecular machenism of TSA on B-NHL. Methods: The cell proliferation was measured by MTT assay. The apoptosis rates and cell cycle were determined with flow cytometry. The expression of hGCN5 in treated Daudi cells was determined by immunocytochemistry and Western blot. Results: The proliferation of Daudi cells was decreased in TSA-treated group. Treatment with TSA(200μg/L) and TSA (400μg/L) for 24h, the apoptosis rates of Daudi cells were (14.74±2.04)% and(17.63±1.25)% respectively. The cell cycle was arrested in G0/G1 phase (50, 100μg/L) and in G2/M phase (200μg/L) for 24h. The expression of hGCN5 in Daudi cells was increased in TSA group (400μg/L) for 24h compared with control group by immunocytochemistry and Western blot (P<0.05). Conclusion: TSA can increase the expression of hGCN5 in Daudi cells and it plays an important role in regulating B-NHL cell line Daudi cells proliferation effects.