File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1007/s12185-009-0311-8
- Scopus: eid_2-s2.0-67749107960
- PMID: 19365708
- WOS: WOS:000266583200011
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Involvement of SRC-3 in deguelin-induced apoptosis in Jurkat cells
Title | Involvement of SRC-3 in deguelin-induced apoptosis in Jurkat cells | ||||
---|---|---|---|---|---|
Authors | |||||
Keywords | Chemicals And Cas Registry Numbers | ||||
Issue Date | 2009 | ||||
Publisher | Springer Japan KK | ||||
Citation | International Journal of Hematology, 2009, v. 89 n. 5, p. 628-635 How to Cite? | ||||
Abstract | The aim of the study was to investigate the anticancer effects and the molecular mechanisms of deguelin on Jurkat cells. Cell viability was assessed by MTT assay. Terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) assay and transmission electron microscopy were used to detect cell apoptosis. A propidium iodide method was used to study cell cycle distribution. RT-PCR and Western blotting were employed to assess the expression levels of steroid receptor coactivator-3 (SRC-3), nuclear factor-κB (NF-κB) and some apoptosis related genes, including Bcl-2 and Bcl-xL. Deguelin was able to inhibit cell proliferation by a cell-cycle arrest in the G1/G 0 phase and induce apoptosis in Jurkat cells in vitro, with a 24-h IC50 value of 43.73 ± 0.35 nmol/L. The antileukemia effect of deguelin might be correlated well with the downregulation of the expression of SRC-3 and its related transcription factor NF-κB, which thus influenced the expression of apoptosis related genes Bcl-2 and Bcl-xL. Deguelin presented potent effects on growth arrest and apoptosis induction in Jurkat cells in vitro via the interruption of SRC-3. © 2009 The Japanese Society of Hematology. | ||||
Persistent Identifier | http://hdl.handle.net/10722/92196 | ||||
ISSN | 2023 Impact Factor: 1.7 2023 SCImago Journal Rankings: 0.594 | ||||
ISI Accession Number ID |
Funding Information: This work was supported by a grant from the National Natural Sciences Foundation of China ( no. 30472267). The authors would like to thank the Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China, for offering relevant experimental facilities and technical support. | ||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, R | en_HK |
dc.contributor.author | Chen, Y | en_HK |
dc.contributor.author | Shu, W-X | en_HK |
dc.contributor.author | Chen, Z | en_HK |
dc.contributor.author | Ke, W-J | en_HK |
dc.date.accessioned | 2010-09-17T10:38:54Z | - |
dc.date.available | 2010-09-17T10:38:54Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | International Journal of Hematology, 2009, v. 89 n. 5, p. 628-635 | en_HK |
dc.identifier.issn | 0925-5710 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/92196 | - |
dc.description.abstract | The aim of the study was to investigate the anticancer effects and the molecular mechanisms of deguelin on Jurkat cells. Cell viability was assessed by MTT assay. Terminal deoxynucleotide transferase-mediated dUTP nick end labeling (TUNEL) assay and transmission electron microscopy were used to detect cell apoptosis. A propidium iodide method was used to study cell cycle distribution. RT-PCR and Western blotting were employed to assess the expression levels of steroid receptor coactivator-3 (SRC-3), nuclear factor-κB (NF-κB) and some apoptosis related genes, including Bcl-2 and Bcl-xL. Deguelin was able to inhibit cell proliferation by a cell-cycle arrest in the G1/G 0 phase and induce apoptosis in Jurkat cells in vitro, with a 24-h IC50 value of 43.73 ± 0.35 nmol/L. The antileukemia effect of deguelin might be correlated well with the downregulation of the expression of SRC-3 and its related transcription factor NF-κB, which thus influenced the expression of apoptosis related genes Bcl-2 and Bcl-xL. Deguelin presented potent effects on growth arrest and apoptosis induction in Jurkat cells in vitro via the interruption of SRC-3. © 2009 The Japanese Society of Hematology. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Springer Japan KK | en_HK |
dc.relation.ispartof | International Journal of Hematology | en_HK |
dc.subject | Chemicals And Cas Registry Numbers | en_HK |
dc.title | Involvement of SRC-3 in deguelin-induced apoptosis in Jurkat cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Chen, Y:ychenc@hkucc.hku.hk | en_HK |
dc.identifier.authority | Chen, Y=rp1318 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s12185-009-0311-8 | en_HK |
dc.identifier.pmid | 19365708 | - |
dc.identifier.scopus | eid_2-s2.0-67749107960 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-67749107960&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 89 | en_HK |
dc.identifier.issue | 5 | en_HK |
dc.identifier.spage | 628 | en_HK |
dc.identifier.epage | 635 | en_HK |
dc.identifier.isi | WOS:000266583200011 | - |
dc.identifier.citeulike | 4341363 | - |
dc.identifier.issnl | 0925-5710 | - |