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- Publisher Website: 10.1016/j.bbrc.2008.09.076
- Scopus: eid_2-s2.0-54849442444
- PMID: 18823947
- WOS: WOS:000260738500009
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Article: Functional consequences of overexpressing the gap junction Cx43 in the cardiogenic potential of pluripotent human embryonic stem cells
Title | Functional consequences of overexpressing the gap junction Cx43 in the cardiogenic potential of pluripotent human embryonic stem cells | ||||||
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Authors | |||||||
Keywords | Cardiac differentiation Connexin Human embryonic stem cells Pluripotency | ||||||
Issue Date | 2008 | ||||||
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description | ||||||
Citation | Biochemical And Biophysical Research Communications, 2008, v. 377 n. 1, p. 46-51 How to Cite? | ||||||
Abstract | Gap junctions, encoded by the connexin (Cx) multi-gene family, couple adjacent cells and underlie cell-cell communications. Previous mouse studies suggest that Cxs play an important role in development but their role in human cardiogenesis is undefined. Human embryonic stem cells (hESC) provide a unique model for studying human differentiation. Lentivirus-mediated stable overexpression of Cx43 in hESC (Cx43-hESC) did not affect colony morphology, karyotype and expression of pluripotency genes such as Oct4 but completely suppressed the formation of spontaneously beating, cardiomyocyte-containing clusters in embryoid bodies (EBs). Unlike control hEBs, the transcripts of several mesodermal markers (kallikrein, δ-globin, and CMP), ventricular myosin light chain and cardiac troponin I were absent or delayed. Transcriptomic and pathway analyses showed that 194 genes crucial for movement, growth, differentiation and maintenance were differentially expressed in Cx43-hESC. We conclude that Cx43 mediates the expression of an array of genes involved in human cardiogenesis, in addition to intercellular communication. © 2008 Elsevier Inc. All rights reserved. | ||||||
Persistent Identifier | http://hdl.handle.net/10722/91625 | ||||||
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.770 | ||||||
ISI Accession Number ID |
Funding Information: This work was supported by grants from the National Institutes of Health (R01 HL-72857 to R.A.L. and F32 HL078330 to J.C.M) and the Hong Kong Research Grant Council (HKU 7633/06M to H.F.T., and R.A.L). | ||||||
References | |||||||
Grants |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Moore, JC | en_HK |
dc.contributor.author | Tsang, SY | en_HK |
dc.contributor.author | Rushing, SN | en_HK |
dc.contributor.author | Lin, D | en_HK |
dc.contributor.author | Tse, HF | en_HK |
dc.contributor.author | Chan, CWY | en_HK |
dc.contributor.author | Li, RA | en_HK |
dc.date.accessioned | 2010-09-17T10:22:24Z | - |
dc.date.available | 2010-09-17T10:22:24Z | - |
dc.date.issued | 2008 | en_HK |
dc.identifier.citation | Biochemical And Biophysical Research Communications, 2008, v. 377 n. 1, p. 46-51 | en_HK |
dc.identifier.issn | 0006-291X | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/91625 | - |
dc.description.abstract | Gap junctions, encoded by the connexin (Cx) multi-gene family, couple adjacent cells and underlie cell-cell communications. Previous mouse studies suggest that Cxs play an important role in development but their role in human cardiogenesis is undefined. Human embryonic stem cells (hESC) provide a unique model for studying human differentiation. Lentivirus-mediated stable overexpression of Cx43 in hESC (Cx43-hESC) did not affect colony morphology, karyotype and expression of pluripotency genes such as Oct4 but completely suppressed the formation of spontaneously beating, cardiomyocyte-containing clusters in embryoid bodies (EBs). Unlike control hEBs, the transcripts of several mesodermal markers (kallikrein, δ-globin, and CMP), ventricular myosin light chain and cardiac troponin I were absent or delayed. Transcriptomic and pathway analyses showed that 194 genes crucial for movement, growth, differentiation and maintenance were differentially expressed in Cx43-hESC. We conclude that Cx43 mediates the expression of an array of genes involved in human cardiogenesis, in addition to intercellular communication. © 2008 Elsevier Inc. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description | en_HK |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | en_HK |
dc.subject | Cardiac differentiation | en_HK |
dc.subject | Connexin | en_HK |
dc.subject | Human embryonic stem cells | en_HK |
dc.subject | Pluripotency | en_HK |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Cell Communication - genetics | en_HK |
dc.subject.mesh | Cell Differentiation - genetics | en_HK |
dc.subject.mesh | Cell Line | en_HK |
dc.subject.mesh | Connexin 43 - genetics - metabolism | en_HK |
dc.subject.mesh | Embryonic Stem Cells - cytology - metabolism - physiology | en_HK |
dc.subject.mesh | Gap Junctions - metabolism | en_HK |
dc.subject.mesh | Gene Expression Regulation, Developmental | en_HK |
dc.subject.mesh | Heart - embryology | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Myocytes, Cardiac - cytology | en_HK |
dc.subject.mesh | Organogenesis - genetics | en_HK |
dc.subject.mesh | Pluripotent Stem Cells - cytology - metabolism - physiology | en_HK |
dc.subject.mesh | Rats | en_HK |
dc.subject.mesh | Transcription, Genetic | en_HK |
dc.title | Functional consequences of overexpressing the gap junction Cx43 in the cardiogenic potential of pluripotent human embryonic stem cells | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Tse, HF:hftse@hkucc.hku.hk | en_HK |
dc.identifier.email | Chan, CWY:camchan@hku.hk | en_HK |
dc.identifier.email | Li, RA:ronaldli@hkucc.hku.hk | en_HK |
dc.identifier.authority | Tse, HF=rp00428 | en_HK |
dc.identifier.authority | Chan, CWY=rp01311 | en_HK |
dc.identifier.authority | Li, RA=rp01352 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.bbrc.2008.09.076 | en_HK |
dc.identifier.pmid | 18823947 | - |
dc.identifier.scopus | eid_2-s2.0-54849442444 | en_HK |
dc.identifier.hkuros | 158692 | - |
dc.identifier.hkuros | 222566 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-54849442444&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 377 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 46 | en_HK |
dc.identifier.epage | 51 | en_HK |
dc.identifier.eissn | 1090-2104 | - |
dc.identifier.isi | WOS:000260738500009 | - |
dc.publisher.place | United States | en_HK |
dc.relation.project | Genetic enrichment of cardiac derivatives from human embryonic stem cells and their bioengineering for cell-based heart therapies | - |
dc.identifier.scopusauthorid | Moore, JC=35185459800 | en_HK |
dc.identifier.scopusauthorid | Tsang, SY=7102255908 | en_HK |
dc.identifier.scopusauthorid | Rushing, SN=25121769500 | en_HK |
dc.identifier.scopusauthorid | Lin, D=8905703100 | en_HK |
dc.identifier.scopusauthorid | Tse, HF=7006070805 | en_HK |
dc.identifier.scopusauthorid | Chan, CWY=12240386600 | en_HK |
dc.identifier.scopusauthorid | Li, RA=7404724466 | en_HK |
dc.identifier.citeulike | 6254615 | - |
dc.identifier.issnl | 0006-291X | - |