File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Characterization of ionic currents in human mesenchymal stem cells from bone marrow

TitleCharacterization of ionic currents in human mesenchymal stem cells from bone marrow
Authors
KeywordsCa2+-activated K+ current
Heag K+ current
Human mesenchymal stem cells
Ion channels
Transient outward K+ current
TTX-sensitive Na+ current
Issue Date2005
PublisherAlphaMed Press, Inc. The Journal's web site is located at http://www.stemcells.com
Citation
Stem Cells, 2005, v. 23 n. 3, p. 371-382 How to Cite?
AbstractThis study characterized functional ion channels in cultured undifferentiated human mesenchymal stem cells (hMSCs) from bone marrow with whole-cell patch clamp and reverse transcription polymerase chain reaction (RT-PCR) techniques. Three types of outward currents were found in hMSCs, including a noise-like rapidly activating outward current inhibited by the large conductance Ca2+-activated K+ channel (IKCa) blocker iberiotoxin, a transient outward K+ current (Ito) suppressed by 4-aminopyridine (4-AP), and a delayed rectifier K+ current (IKDR)-like ether-à-go-go (eag) K+ channel. In addition, tetrodotoxin-sensitive sodium current (INa.TTX) and nifedipine-sensitive L-type Ca2+ current (ICa.L) were also detected in 29% and 15% hMSCs, respectively. Moreover, RT-PCR revealed the molecular evidence of high levels of mRNA for the functional ionic currents, including human MaxiK for IKCa, Kv4.2 and Kv1.4 for Ito, heag1 for IKDR, hNE-Na for INa.TTX, and CACNAIC for I Ca.L. These results demonstrate that multiple functional ion channel currents-that is, IKca, Ito heag1, INa.TTX, and ICa.L-are expressed in hMSCs from bone marrow.
Persistent Identifierhttp://hdl.handle.net/10722/85513
ISSN
2023 Impact Factor: 4.0
2023 SCImago Journal Rankings: 1.396
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, GRen_HK
dc.contributor.authorSun, Hen_HK
dc.contributor.authorDeng, Xen_HK
dc.contributor.authorLau, CPen_HK
dc.date.accessioned2010-09-06T09:05:57Z-
dc.date.available2010-09-06T09:05:57Z-
dc.date.issued2005en_HK
dc.identifier.citationStem Cells, 2005, v. 23 n. 3, p. 371-382en_HK
dc.identifier.issn1066-5099en_HK
dc.identifier.urihttp://hdl.handle.net/10722/85513-
dc.description.abstractThis study characterized functional ion channels in cultured undifferentiated human mesenchymal stem cells (hMSCs) from bone marrow with whole-cell patch clamp and reverse transcription polymerase chain reaction (RT-PCR) techniques. Three types of outward currents were found in hMSCs, including a noise-like rapidly activating outward current inhibited by the large conductance Ca2+-activated K+ channel (IKCa) blocker iberiotoxin, a transient outward K+ current (Ito) suppressed by 4-aminopyridine (4-AP), and a delayed rectifier K+ current (IKDR)-like ether-à-go-go (eag) K+ channel. In addition, tetrodotoxin-sensitive sodium current (INa.TTX) and nifedipine-sensitive L-type Ca2+ current (ICa.L) were also detected in 29% and 15% hMSCs, respectively. Moreover, RT-PCR revealed the molecular evidence of high levels of mRNA for the functional ionic currents, including human MaxiK for IKCa, Kv4.2 and Kv1.4 for Ito, heag1 for IKDR, hNE-Na for INa.TTX, and CACNAIC for I Ca.L. These results demonstrate that multiple functional ion channel currents-that is, IKca, Ito heag1, INa.TTX, and ICa.L-are expressed in hMSCs from bone marrow.en_HK
dc.languageengen_HK
dc.publisherAlphaMed Press, Inc. The Journal's web site is located at http://www.stemcells.comen_HK
dc.relation.ispartofStem Cellsen_HK
dc.subjectCa2+-activated K+ current-
dc.subjectHeag K+ current-
dc.subjectHuman mesenchymal stem cells-
dc.subjectIon channels-
dc.subjectTransient outward K+ current-
dc.subjectTTX-sensitive Na+ current-
dc.subject.mesh4-Aminopyridine - pharmacologyen_HK
dc.subject.meshBone Marrow Cells - cytology - drug effects - physiologyen_HK
dc.subject.meshCalcium Channels, L-Type - drug effects - genetics - physiologyen_HK
dc.subject.meshCell Differentiationen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshEther-A-Go-Go Potassium Channelsen_HK
dc.subject.meshGene Expression - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIon Channels - genetics - physiologyen_HK
dc.subject.meshKv1.4 Potassium Channelen_HK
dc.subject.meshLarge-Conductance Calcium-Activated Potassium Channel alpha Subunitsen_HK
dc.subject.meshLarge-Conductance Calcium-Activated Potassium Channelsen_HK
dc.subject.meshMembrane Potentials - drug effectsen_HK
dc.subject.meshMesenchymal Stem Cells - cytology - drug effects - physiologyen_HK
dc.subject.meshNerve Tissue Proteins - geneticsen_HK
dc.subject.meshNifedipine - pharmacologyen_HK
dc.subject.meshPatch-Clamp Techniquesen_HK
dc.subject.meshPeptides - pharmacologyen_HK
dc.subject.meshPotassium Channels - geneticsen_HK
dc.subject.meshPotassium Channels, Calcium-Activated - drug effects - genetics - physiologyen_HK
dc.subject.meshPotassium Channels, Voltage-Gated - drug effects - genetics - physiologyen_HK
dc.subject.meshRNA, Messenger - genetics - metabolismen_HK
dc.subject.meshShal Potassium Channelsen_HK
dc.subject.meshSodium Channels - drug effects - genetics - physiologyen_HK
dc.subject.meshTetrodotoxin - pharmacologyen_HK
dc.titleCharacterization of ionic currents in human mesenchymal stem cells from bone marrowen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1066-5099&volume=23&issue=3&spage=371&epage=382&date=2005&atitle=Characterization+of+ionic+currents+in+human+mesenchymal+stem+cells+from+bone+marrowen_HK
dc.identifier.emailLi, GR:grli@hkucc.hku.hken_HK
dc.identifier.authorityLi, GR=rp00476en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1634/stemcells.2004-0213en_HK
dc.identifier.pmid15749932-
dc.identifier.scopuseid_2-s2.0-15544379480en_HK
dc.identifier.hkuros101185en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-15544379480&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume23en_HK
dc.identifier.issue3en_HK
dc.identifier.spage371en_HK
dc.identifier.epage382en_HK
dc.identifier.isiWOS:000227786900010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, GR=7408462932en_HK
dc.identifier.scopusauthoridSun, H=35723049200en_HK
dc.identifier.scopusauthoridDeng, X=14057894600en_HK
dc.identifier.scopusauthoridLau, CP=7401968501en_HK
dc.identifier.issnl1066-5099-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats