File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Stability of insulin-like growth factor I in the gastrointestinal lumen in neonatal pigs

TitleStability of insulin-like growth factor I in the gastrointestinal lumen in neonatal pigs
Authors
KeywordsColostrum
Gastrointestinal tract
Insulin-like growth factor- I
Stability
Suckling pigs
Issue Date2000
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.jpgn.org
Citation
Journal Of Pediatric Gastroenterology And Nutrition, 2000, v. 30 n. 3, p. 299-304 How to Cite?
AbstractBackground: Insulin-like growth factor (IGF)-I is present in the milk of various species. A prerequisite for any biological activity of milk-borne IGF-I in the suckling young is to survive the gastrointestinal luminal digestion. In the present study, the stability of IGF-I was examined in the gastrointestinal lumen in neonatal pigs. Methods: Iodine-labeled IGF-I was incubated in the gastrointestinal luminal fluids of 3-day-old suckling and 45-day-old weaned pigs at 37°C for 20 minutes. Degradation of the peptide was analyzed by trichloroacetic acid (TCA) precipitation, liquid chromatography, and receptor binding assay. Results: IGF-I remained unchanged in the gastric fluids of suckling and weaned pigs when determined by TCA precipitation. IGF-I degraded 3%, 18%, and 37% in the luminal fluids of the proximal, mid and distal small intestine in suckling piglets compared with 53%, 62%, and 54% in weaned pigs. The results were supported by the chromatography and receptor binding analysis. Porcine colostrum had a capacity to protect IGF-I from gastrointestinal luminal digestion in weaned pigs. Conclusion: Milk-borne IGF-I is stable in the gastrointestinal lumen in suckling pigs and may play a role in regulating postnatal development in the suckling young. (C) 2000 Lippincott Williams and Wilkins, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/84649
ISSN
2023 Impact Factor: 2.4
2023 SCImago Journal Rankings: 0.837
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShen, WHen_HK
dc.contributor.authorXu, RJen_HK
dc.date.accessioned2010-09-06T08:55:30Z-
dc.date.available2010-09-06T08:55:30Z-
dc.date.issued2000en_HK
dc.identifier.citationJournal Of Pediatric Gastroenterology And Nutrition, 2000, v. 30 n. 3, p. 299-304en_HK
dc.identifier.issn0277-2116en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84649-
dc.description.abstractBackground: Insulin-like growth factor (IGF)-I is present in the milk of various species. A prerequisite for any biological activity of milk-borne IGF-I in the suckling young is to survive the gastrointestinal luminal digestion. In the present study, the stability of IGF-I was examined in the gastrointestinal lumen in neonatal pigs. Methods: Iodine-labeled IGF-I was incubated in the gastrointestinal luminal fluids of 3-day-old suckling and 45-day-old weaned pigs at 37°C for 20 minutes. Degradation of the peptide was analyzed by trichloroacetic acid (TCA) precipitation, liquid chromatography, and receptor binding assay. Results: IGF-I remained unchanged in the gastric fluids of suckling and weaned pigs when determined by TCA precipitation. IGF-I degraded 3%, 18%, and 37% in the luminal fluids of the proximal, mid and distal small intestine in suckling piglets compared with 53%, 62%, and 54% in weaned pigs. The results were supported by the chromatography and receptor binding analysis. Porcine colostrum had a capacity to protect IGF-I from gastrointestinal luminal digestion in weaned pigs. Conclusion: Milk-borne IGF-I is stable in the gastrointestinal lumen in suckling pigs and may play a role in regulating postnatal development in the suckling young. (C) 2000 Lippincott Williams and Wilkins, Inc.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.jpgn.orgen_HK
dc.relation.ispartofJournal of Pediatric Gastroenterology and Nutritionen_HK
dc.rightsJournal of Pediatric Gastroenterology and Nutrition. Copyright © Lippincott Williams & Wilkins.en_HK
dc.subjectColostrumen_HK
dc.subjectGastrointestinal tracten_HK
dc.subjectInsulin-like growth factor- Ien_HK
dc.subjectStabilityen_HK
dc.subjectSuckling pigsen_HK
dc.titleStability of insulin-like growth factor I in the gastrointestinal lumen in neonatal pigsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0277-2116&volume=30&spage=299&epage=304&date=2000&atitle=Stability+of+insulin-like+growth+factor+I+in+the+gastrointestinal+lumen+in+neonatal+pigsen_HK
dc.identifier.emailXu, RJ: xuruojun@hkucc.hku.hken_HK
dc.identifier.authorityXu, RJ=rp00820en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1097/00005176-200003000-00016en_HK
dc.identifier.pmid10749415-
dc.identifier.scopuseid_2-s2.0-0034096913en_HK
dc.identifier.hkuros48315en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034096913&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume30en_HK
dc.identifier.issue3en_HK
dc.identifier.spage299en_HK
dc.identifier.epage304en_HK
dc.identifier.isiWOS:000085914800016-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridShen, WH=46261460000en_HK
dc.identifier.scopusauthoridXu, RJ=7402813973en_HK
dc.identifier.issnl0277-2116-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats