File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/j.jep.2009.03.005
- Scopus: eid_2-s2.0-67349155604
- PMID: 19501992
- WOS: WOS:000268201200018
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Therapeutic efficacy of Traditional Chinese Medicine 319 recipe on hepatic fibrosis induced by carbon tetrachloride in rats
Title | Therapeutic efficacy of Traditional Chinese Medicine 319 recipe on hepatic fibrosis induced by carbon tetrachloride in rats | ||||||
---|---|---|---|---|---|---|---|
Authors | |||||||
Keywords | Fuzheng Huayu Recipe Hepatic fibrosis Hepatic stellate cell PDGF Radix Salviae Miltiorrhizae TGF-β1 TIMP-1 Traditional Chinese Medicine | ||||||
Issue Date | 2009 | ||||||
Publisher | Elsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm | ||||||
Citation | Journal Of Ethnopharmacology, 2009, v. 124 n. 1, p. 142-150 How to Cite? | ||||||
Abstract | Background/aims: Hepatic fibrosis is a consequence of severe liver damage that occurs in many patients with chronic liver diseases. TCM 319 recipe is a Chinese Medicine formula which consists of six Chinese herbs. In this study, we investigated the anti-fibrotic efficacy and mechanisms of TCM 319 recipe. Methods: Hepatic fibrosis in rats was induced by carbon tetrachloride (CCl4). 34 male adult SD rats were allocated into five groups (group 1-concomitant CCl4 and TCM 319 recipe for 8 weeks; group 2-CCl4 for 4 weeks and then CCl4 and TCM 319 recipe for 4 weeks; group 3-CCl4 alone for 8 weeks; group 4-TCM 319 recipe only for 8 weeks; group 5-untreated controls). After 8 weeks of treatment, serum ALT assay, liver tissue histological examination and immunostaining were carried out to examine the liver function and fibrosis degree. The expression levels of platelet derived growth factor (PDGF-B), PDGF-Rβ, and transforming growth factor-beta 1 (TGF-β1) were measured by quantitative RT-PCR and western blot. Results: TCM 319 recipe reduced liver injury and attenuated hepatic fibrosis in group 1 compared with that in group 3. TCM 319 recipe suppressed the mRNA expression of tissue inhibitor of metalloproteinase 1 (TIMP-1). In addition, treatment with TCM 319 recipe significantly down-regulated mRNA expression of PDGF-B and PDGF-Rβ, and it also suppressed protein expression of PDGF-Rβ and TGF-β1. Conclusions: TCM 319 recipe extracts could attenuate hepatic fibrosis induced by CCl4 in rats. The anti-fibrotic effect of TCM 319 recipe is associated with the down-regulation of mRNA expression of TIMP-1, PDGF-B and PDGF-Rβ, and with the suppression of protein expression of PDGF-Rβ and TGF-β1. © 2009 Elsevier Ireland Ltd. All rights reserved. | ||||||
Persistent Identifier | http://hdl.handle.net/10722/83425 | ||||||
ISSN | 2021 Impact Factor: 5.195 2020 SCImago Journal Rankings: 0.885 | ||||||
ISI Accession Number ID |
Funding Information: This study was supported by Research Grant Council to Prof. George K.K. Lau (Grant number: HKU 7679/06M) and Cheng Si Yuan (China-International) Hepatitis Research Foundation. | ||||||
References | |||||||
Grants |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cheung, KF | en_HK |
dc.contributor.author | Ye, DW | en_HK |
dc.contributor.author | Yang, ZF | en_HK |
dc.contributor.author | Lu, L | en_HK |
dc.contributor.author | Liu, CH | en_HK |
dc.contributor.author | Wang, XL | en_HK |
dc.contributor.author | Poon, RTP | en_HK |
dc.contributor.author | Tong, Y | en_HK |
dc.contributor.author | Liu, P | en_HK |
dc.contributor.author | Chen, YC | en_HK |
dc.contributor.author | Lau, GKK | en_HK |
dc.date.accessioned | 2010-09-06T08:40:53Z | - |
dc.date.available | 2010-09-06T08:40:53Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | Journal Of Ethnopharmacology, 2009, v. 124 n. 1, p. 142-150 | en_HK |
dc.identifier.issn | 0378-8741 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/83425 | - |
dc.description.abstract | Background/aims: Hepatic fibrosis is a consequence of severe liver damage that occurs in many patients with chronic liver diseases. TCM 319 recipe is a Chinese Medicine formula which consists of six Chinese herbs. In this study, we investigated the anti-fibrotic efficacy and mechanisms of TCM 319 recipe. Methods: Hepatic fibrosis in rats was induced by carbon tetrachloride (CCl4). 34 male adult SD rats were allocated into five groups (group 1-concomitant CCl4 and TCM 319 recipe for 8 weeks; group 2-CCl4 for 4 weeks and then CCl4 and TCM 319 recipe for 4 weeks; group 3-CCl4 alone for 8 weeks; group 4-TCM 319 recipe only for 8 weeks; group 5-untreated controls). After 8 weeks of treatment, serum ALT assay, liver tissue histological examination and immunostaining were carried out to examine the liver function and fibrosis degree. The expression levels of platelet derived growth factor (PDGF-B), PDGF-Rβ, and transforming growth factor-beta 1 (TGF-β1) were measured by quantitative RT-PCR and western blot. Results: TCM 319 recipe reduced liver injury and attenuated hepatic fibrosis in group 1 compared with that in group 3. TCM 319 recipe suppressed the mRNA expression of tissue inhibitor of metalloproteinase 1 (TIMP-1). In addition, treatment with TCM 319 recipe significantly down-regulated mRNA expression of PDGF-B and PDGF-Rβ, and it also suppressed protein expression of PDGF-Rβ and TGF-β1. Conclusions: TCM 319 recipe extracts could attenuate hepatic fibrosis induced by CCl4 in rats. The anti-fibrotic effect of TCM 319 recipe is associated with the down-regulation of mRNA expression of TIMP-1, PDGF-B and PDGF-Rβ, and with the suppression of protein expression of PDGF-Rβ and TGF-β1. © 2009 Elsevier Ireland Ltd. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jethpharm | en_HK |
dc.relation.ispartof | Journal of Ethnopharmacology | en_HK |
dc.rights | Journal of Ethnopharmacology. Copyright © Elsevier Ireland Ltd. | en_HK |
dc.subject | Fuzheng Huayu Recipe | en_HK |
dc.subject | Hepatic fibrosis | en_HK |
dc.subject | Hepatic stellate cell | en_HK |
dc.subject | PDGF | en_HK |
dc.subject | Radix Salviae Miltiorrhizae | en_HK |
dc.subject | TGF-β1 | en_HK |
dc.subject | TIMP-1 | en_HK |
dc.subject | Traditional Chinese Medicine | en_HK |
dc.subject.mesh | Actins - metabolism | en_HK |
dc.subject.mesh | Alanine Transaminase - metabolism | en_HK |
dc.subject.mesh | Angiosperms | en_HK |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Carbon Tetrachloride | en_HK |
dc.subject.mesh | Collagen - metabolism | en_HK |
dc.subject.mesh | Down-Regulation | en_HK |
dc.subject.mesh | Drug Combinations | en_HK |
dc.subject.mesh | Drugs, Chinese Herbal - pharmacology - therapeutic use | en_HK |
dc.subject.mesh | Hepatic Stellate Cells - metabolism | en_HK |
dc.subject.mesh | Liver - drug effects - metabolism - pathology | en_HK |
dc.subject.mesh | Liver Cirrhosis - chemically induced - metabolism - prevention & control | en_HK |
dc.subject.mesh | Male | en_HK |
dc.subject.mesh | Medicine, Chinese Traditional | en_HK |
dc.subject.mesh | Phytotherapy | en_HK |
dc.subject.mesh | Platelet-Derived Growth Factor - genetics - metabolism | en_HK |
dc.subject.mesh | RNA, Messenger - metabolism | en_HK |
dc.subject.mesh | Rats | en_HK |
dc.subject.mesh | Rats, Sprague-Dawley | en_HK |
dc.subject.mesh | Tissue Inhibitor of Metalloproteinase-1 - genetics - metabolism | en_HK |
dc.subject.mesh | Transforming Growth Factor beta1 - metabolism | en_HK |
dc.title | Therapeutic efficacy of Traditional Chinese Medicine 319 recipe on hepatic fibrosis induced by carbon tetrachloride in rats | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0378-8741&volume=124&issue=1&spage=142&epage=150&date=2009&atitle=Therapeutic+efficacy+of+Traditional+Chinese+Medicine+319+recipe+on+hepatic+fibrosis+induced+by+carbon+tetrachloride+in+rats | en_HK |
dc.identifier.email | Lu, L: liweilu@hkucc.hku.hk | en_HK |
dc.identifier.email | Poon, RTP: poontp@hku.hk | en_HK |
dc.identifier.email | Tong, Y: tongyao@hku.hk | en_HK |
dc.identifier.authority | Lu, L=rp00477 | en_HK |
dc.identifier.authority | Poon, RTP=rp00446 | en_HK |
dc.identifier.authority | Tong, Y=rp00509 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.jep.2009.03.005 | en_HK |
dc.identifier.pmid | 19501992 | - |
dc.identifier.scopus | eid_2-s2.0-67349155604 | en_HK |
dc.identifier.hkuros | 167040 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-67349155604&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 124 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 142 | en_HK |
dc.identifier.epage | 150 | en_HK |
dc.identifier.isi | WOS:000268201200018 | - |
dc.publisher.place | Ireland | en_HK |
dc.relation.project | Role of upregulation of toll-like receptor 7 and functional polymorphisms within the toll-like receptor 7 gene in inducing sustained disease remission in chronic hepatitis B infection | - |
dc.identifier.scopusauthorid | Cheung, KF=8763216400 | en_HK |
dc.identifier.scopusauthorid | Ye, DW=35277778400 | en_HK |
dc.identifier.scopusauthorid | Yang, ZF=14018809600 | en_HK |
dc.identifier.scopusauthorid | Lu, L=7403963552 | en_HK |
dc.identifier.scopusauthorid | Liu, CH=36064322500 | en_HK |
dc.identifier.scopusauthorid | Wang, XL=24478987700 | en_HK |
dc.identifier.scopusauthorid | Poon, RTP=7103097223 | en_HK |
dc.identifier.scopusauthorid | Tong, Y=9045384000 | en_HK |
dc.identifier.scopusauthorid | Liu, P=36065603800 | en_HK |
dc.identifier.scopusauthorid | Chen, YC=35227073300 | en_HK |
dc.identifier.scopusauthorid | Lau, GKK=7102301257 | en_HK |
dc.identifier.issnl | 0378-8741 | - |