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- Publisher Website: 10.1016/S0024-3205(02)01472-8
- Scopus: eid_2-s2.0-0036498591
- PMID: 12002524
- WOS: WOS:000174853600008
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Article: Forskolin fails to activate L-type calcium current in hypertrophied cardiomyocytes of chronically hypoxic rats
Title | Forskolin fails to activate L-type calcium current in hypertrophied cardiomyocytes of chronically hypoxic rats |
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Authors | |
Keywords | β-adrenoceptor receptors Adenylyl cyclase Calcium current cAMP Cardiomyocyte Chronic hypoxia Forskolin Hypertrophy |
Issue Date | 2002 |
Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie |
Citation | Life Sciences, 2002, v. 70 n. 15, p. 1801-1809 How to Cite? |
Abstract | We have shown that the contractile, cytosolic calcium ([Ca2+]i) and cyclic AMP (cAMP) responses to β-adrenoceptor stimulation are attenuated in ventricular myocytes of chronically hypoxic (CH) rats. The aim of this study was to examine the effect of forskolin on the L-type Ca2+ current in CH hypertrophied ventricular myocytes. Patch-clamp recording of the L-type Ca2+ current was measured in right ventricular myocytes of normoxic control and CH rats exposed to 10% inspired oxygen for 4 weeks. The breadth, but not the length, of CH myocytes was significantly greater than that of the control group. Activation of β-adrenoceptor with isoproterenol (0.1 μM) increased the peak Ca2+ current by 83% in the normoxic control but the increase of peak Ca2+ current was not significant in the CH myocytes. Forskolin (0.1-1 μM), an activator of adenylyl cyclase, increased the peak Ca2+ current by 49%-102% in the normoxic controls but it did not cause significant change of the peak Ca2+ current in CH myocytes. These results suggest an absence of forskolin-induced activation of Ca2+ current in hypertrophied ventricular myocytes during chronic hypoxia. The failure of activation of the Ca2+ current is consistent with the idea that adenylyl cyclase function is down-regulated in CH hypertrophied myocytes. © 2002 Elsevier Science Inc. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/81342 |
ISSN | 2023 Impact Factor: 5.2 2023 SCImago Journal Rankings: 1.257 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Fung, ML | en_HK |
dc.contributor.author | Li, HY | en_HK |
dc.contributor.author | Wong, TM | en_HK |
dc.date.accessioned | 2010-09-06T08:16:33Z | - |
dc.date.available | 2010-09-06T08:16:33Z | - |
dc.date.issued | 2002 | en_HK |
dc.identifier.citation | Life Sciences, 2002, v. 70 n. 15, p. 1801-1809 | en_HK |
dc.identifier.issn | 0024-3205 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/81342 | - |
dc.description.abstract | We have shown that the contractile, cytosolic calcium ([Ca2+]i) and cyclic AMP (cAMP) responses to β-adrenoceptor stimulation are attenuated in ventricular myocytes of chronically hypoxic (CH) rats. The aim of this study was to examine the effect of forskolin on the L-type Ca2+ current in CH hypertrophied ventricular myocytes. Patch-clamp recording of the L-type Ca2+ current was measured in right ventricular myocytes of normoxic control and CH rats exposed to 10% inspired oxygen for 4 weeks. The breadth, but not the length, of CH myocytes was significantly greater than that of the control group. Activation of β-adrenoceptor with isoproterenol (0.1 μM) increased the peak Ca2+ current by 83% in the normoxic control but the increase of peak Ca2+ current was not significant in the CH myocytes. Forskolin (0.1-1 μM), an activator of adenylyl cyclase, increased the peak Ca2+ current by 49%-102% in the normoxic controls but it did not cause significant change of the peak Ca2+ current in CH myocytes. These results suggest an absence of forskolin-induced activation of Ca2+ current in hypertrophied ventricular myocytes during chronic hypoxia. The failure of activation of the Ca2+ current is consistent with the idea that adenylyl cyclase function is down-regulated in CH hypertrophied myocytes. © 2002 Elsevier Science Inc. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/lifescie | en_HK |
dc.relation.ispartof | Life Sciences | en_HK |
dc.rights | Life Sciences. Copyright © Elsevier Inc. | en_HK |
dc.subject | β-adrenoceptor receptors | en_HK |
dc.subject | Adenylyl cyclase | en_HK |
dc.subject | Calcium current | en_HK |
dc.subject | cAMP | en_HK |
dc.subject | Cardiomyocyte | en_HK |
dc.subject | Chronic hypoxia | en_HK |
dc.subject | Forskolin | en_HK |
dc.subject | Hypertrophy | en_HK |
dc.title | Forskolin fails to activate L-type calcium current in hypertrophied cardiomyocytes of chronically hypoxic rats | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0024-3205&volume=70 &issue=15&spage=1801&epage=1809&date=2002&atitle=Forskolin+fails+to+activate+L-type+calcium+current+in+hypertrophied+cardiomyocytes+of+chronically+hypoxic+rats | en_HK |
dc.identifier.email | Fung, ML: fungml@hkucc.hku.hk | en_HK |
dc.identifier.authority | Fung, ML=rp00433 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/S0024-3205(02)01472-8 | en_HK |
dc.identifier.pmid | 12002524 | - |
dc.identifier.scopus | eid_2-s2.0-0036498591 | en_HK |
dc.identifier.hkuros | 71915 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0036498591&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 70 | en_HK |
dc.identifier.issue | 15 | en_HK |
dc.identifier.spage | 1801 | en_HK |
dc.identifier.epage | 1809 | en_HK |
dc.identifier.isi | WOS:000174853600008 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Fung, ML=7101955092 | en_HK |
dc.identifier.scopusauthorid | Li, HY=8701136400 | en_HK |
dc.identifier.scopusauthorid | Wong, TM=7403531434 | en_HK |
dc.identifier.issnl | 0024-3205 | - |