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Article: Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis

TitlePlasmid profile and construction of a small shuttle vector in Laribacter hongkongensis
Authors
Woo, PCYMa, SSLTeng, JLLLi, MWSLau, SKPYuen, KY
KeywordsLaribacter hongkongensis
Plasmid profile
Sequence
Shuttle vector
Transformation
Issue Date2007
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0141-5492
Citation
Biotechnology Letters, 2007, v. 29 n. 10, p. 1575-1582 How to Cite?
DOI: http://dx.doi.org/10.1007/s10529-007-9447-x
AbstractAmong 21 human strains of Laribacter hongkongensis, small plasmids were observed in four strains, and large ones in six strains. The smallest, 3264-bp plasmid, pHLHK19, has only one ORF that encodes a putative replication initiator protein and a predicted origin of replication (ori) with a DnaA box, three 18-bp direct repeats and five pairs of inverted repeats. An Escherichia coli-L. hongkongensis shuttle vector was constructed by ligating the HindIII-digested pHLHK19, containing the replication initiator protein and ori of pHLHK19, to HindIII-digested pBK-CMV. This shuttle vector can propagate in E. coli and L. hongkongensis with good transformation efficiencies. © 2007 Springer Science+Business Media B.V.
Persistent Identifierhttp://hdl.handle.net/10722/79191
ISSN
0141-5492
2023 Impact Factor: 2.0
2023 SCImago Journal Rankings: 0.519
ISI Accession Number ID
WOS:000248940500020
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorMa, SSLen_HK
dc.contributor.authorTeng, JLLen_HK
dc.contributor.authorLi, MWSen_HK
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2010-09-06T07:51:42Z-
dc.date.available2010-09-06T07:51:42Z-
dc.date.issued2007en_HK
dc.identifier.citationBiotechnology Letters, 2007, v. 29 n. 10, p. 1575-1582en_HK
dc.identifier.issn0141-5492en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79191-
dc.description.abstractAmong 21 human strains of Laribacter hongkongensis, small plasmids were observed in four strains, and large ones in six strains. The smallest, 3264-bp plasmid, pHLHK19, has only one ORF that encodes a putative replication initiator protein and a predicted origin of replication (ori) with a DnaA box, three 18-bp direct repeats and five pairs of inverted repeats. An Escherichia coli-L. hongkongensis shuttle vector was constructed by ligating the HindIII-digested pHLHK19, containing the replication initiator protein and ori of pHLHK19, to HindIII-digested pBK-CMV. This shuttle vector can propagate in E. coli and L. hongkongensis with good transformation efficiencies. © 2007 Springer Science+Business Media B.V.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0141-5492en_HK
dc.relation.ispartofBiotechnology Lettersen_HK
dc.subjectLaribacter hongkongensis-
dc.subjectPlasmid profile-
dc.subjectSequence-
dc.subjectShuttle vector-
dc.subjectTransformation-
dc.subject.meshBase Sequenceen_HK
dc.subject.meshDNA Helicases - geneticsen_HK
dc.subject.meshDNA-Binding Proteins - geneticsen_HK
dc.subject.meshEscherichia coli - geneticsen_HK
dc.subject.meshGenetic Vectors - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshNeisseriaceae - geneticsen_HK
dc.subject.meshOpen Reading Frames - geneticsen_HK
dc.subject.meshPlasmids - geneticsen_HK
dc.subject.meshReplication Origin - geneticsen_HK
dc.subject.meshTrans-Activators - geneticsen_HK
dc.subject.meshTransformation, Bacterialen_HK
dc.titlePlasmid profile and construction of a small shuttle vector in Laribacter hongkongensisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0141-5492&volume=29&spage=1575&epage=1582&date=2007&atitle=Plasmid+profile+and+construction+of+a+small+shuttle+vector+in+Laribacter+hongkongensisen_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailTeng, JLL:llteng@hku.hken_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityTeng, JLL=rp00277en_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10529-007-9447-xen_HK
dc.identifier.pmid17609856-
dc.identifier.scopuseid_2-s2.0-34548119182en_HK
dc.identifier.hkuros134251en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548119182&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1575en_HK
dc.identifier.epage1582en_HK
dc.identifier.isiWOS:000248940500020-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridMa, SSL=26424668200en_HK
dc.identifier.scopusauthoridTeng, JLL=7202560229en_HK
dc.identifier.scopusauthoridLi, MWS=8967745900en_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.issnl0141-5492-

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