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Article: A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors

TitleA one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors
Authors
Poon, LLMWong, BWYChan, KHLeung, CSWYuen, KYGuan, YPeiris, JSM
KeywordsEarly diagnosis
Quantitative RT-PCR
SARS coronavirus
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
Citation
Journal Of Clinical Virology, 2004, v. 30 n. 3, p. 214-217 How to Cite?
DOI: http://dx.doi.org/10.1016/j.jcv.2003.12.007
AbstractIn this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to PCR inhibitors or faulty RNA extraction, we have further modified this one step RT-PCR assay for simultaneous detection of severe acute respiratory syndrome (SARS) coronavirus (CoV) and 18S ribosomal RNA (rRNA) as an internal positive control. © 2003 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/79143
ISSN
1386-6532
2023 Impact Factor: 4.0
2023 SCImago Journal Rankings: 1.344
ISI Accession Number ID
WOS:000221845900003
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorPoon, LLMen_HK
dc.contributor.authorWong, BWYen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorLeung, CSWen_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.date.accessioned2010-09-06T07:51:07Z-
dc.date.available2010-09-06T07:51:07Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Clinical Virology, 2004, v. 30 n. 3, p. 214-217en_HK
dc.identifier.issn1386-6532en_HK
dc.identifier.urihttp://hdl.handle.net/10722/79143-
dc.description.abstractIn this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to PCR inhibitors or faulty RNA extraction, we have further modified this one step RT-PCR assay for simultaneous detection of severe acute respiratory syndrome (SARS) coronavirus (CoV) and 18S ribosomal RNA (rRNA) as an internal positive control. © 2003 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcven_HK
dc.relation.ispartofJournal of Clinical Virologyen_HK
dc.rightsJournal of clinical virology. Copyright © Elsevier BV.en_HK
dc.subjectEarly diagnosisen_HK
dc.subjectQuantitative RT-PCRen_HK
dc.subjectSARS coronavirusen_HK
dc.subject.meshDNA, Ribosomal - analysisen_HK
dc.subject.meshHumansen_HK
dc.subject.meshNasopharynx - virologyen_HK
dc.subject.meshRNA, Ribosomal, 18S - geneticsen_HK
dc.subject.meshRNA, Viral - analysis - isolation & purificationen_HK
dc.subject.meshReference Standardsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - methodsen_HK
dc.subject.meshSARS Virus - genetics - isolation & purificationen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.subject.meshSevere Acute Respiratory Syndrome - diagnosis - virologyen_HK
dc.titleA one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitorsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1386-6532&volume=30&issue=3&spage=214&epage=7&date=2004&atitle=A+one+step+quantitative+RT-PCR+for+detection+of+SARS+coronavirus+with+an+internal+control+for+PCR+inhibitors.en_HK
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.authorityPoon, LLM=rp00484en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jcv.2003.12.007en_HK
dc.identifier.pmid15135737en_HK
dc.identifier.scopuseid_2-s2.0-2442600174en_HK
dc.identifier.hkuros94837en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2442600174&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume30en_HK
dc.identifier.issue3en_HK
dc.identifier.spage214en_HK
dc.identifier.epage217en_HK
dc.identifier.isiWOS:000221845900003-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridPoon, LLM=7005441747en_HK
dc.identifier.scopusauthoridWong, BWY=8602085500en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridLeung, CSW=7402612654en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.issnl1386-6532-

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