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Article: Association of CTLA-4 gene microsatellite polymorphism with ulcerative colitis in Chinese patients
Title | Association of CTLA-4 gene microsatellite polymorphism with ulcerative colitis in Chinese patients |
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Authors | |
Keywords | Chinese CTLA-4 Genetic polymorphism Ulcerative colitis |
Issue Date | 2006 |
Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.ibdjournal.com |
Citation | Inflammatory Bowel Diseases, 2006, v. 12 n. 5, p. 369-373 How to Cite? |
Abstract | Ulcerative colitis (UC) is characterized by chronic intestinal inflammation as a result of an exaggerated T cell response. Cytotoxic T lymphocyte associated antigen-4 (CTLA-4), expressed mainly in activated T cells, inhibits T cell activation and proliferation by combining B7 through competing CD28 and maintains immune homeostasis. Polymorphisms of the CTLA-4 gene are known to be associated with several autoimmune diseases. The aim of this study was to investigate the association between the CTLA-4 gene micro satellite polymorphism and UC in Chinese patients. Unrelated 100 Chinese patients with UC and 140 healthy controls were studied. The (AT) repeats in the 3′ untranslated region of exon 4 of the CTLA-4 gene were amplified by allele-specific polymerase chain reaction (PCR). The amplified products were electrophoresed on a 12% polyacrylamide gel, followed by silver staining. Twenty alleles were found in Chinese patients and healthy controls. The 122-bp allele was increased in UC compared with healthy controls (9.5% vs 0.7%, P = 0.0001/Pc = 0.002, OR = 14.591, 95% CI 3.357-63.420). The frequency of the longer alleles (≥118 bp) of UC was higher than that in healthy controls (26% vs 4%, P = 0.0001/Pc = 0.0002, OR = 7.644, 95%CI 3.950-14.792), but was not associated with location and severity of the disease. Furthermore, the longer alleles were not associated with haplotypes of C-318T/A+49G of the CTLA-4 gene in Chinese patients with UC. The longer alleles of the CTLA-4 gene microsatellite polymorphism were strongly associated with UC in Chinese patients. Copyright © 2006 by Lippincott Williams & Wilkins. |
Persistent Identifier | http://hdl.handle.net/10722/78391 |
ISSN | 2023 Impact Factor: 4.5 2023 SCImago Journal Rankings: 1.550 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jiang, Y | en_HK |
dc.contributor.author | Xia, B | en_HK |
dc.contributor.author | Jiang, L | en_HK |
dc.contributor.author | Lv, M | en_HK |
dc.contributor.author | Guo, Q | en_HK |
dc.contributor.author | Chen, M | en_HK |
dc.contributor.author | Li, J | en_HK |
dc.contributor.author | Xia, HHX | en_HK |
dc.contributor.author | Wong, BCY | en_HK |
dc.date.accessioned | 2010-09-06T07:42:22Z | - |
dc.date.available | 2010-09-06T07:42:22Z | - |
dc.date.issued | 2006 | en_HK |
dc.identifier.citation | Inflammatory Bowel Diseases, 2006, v. 12 n. 5, p. 369-373 | en_HK |
dc.identifier.issn | 1078-0998 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/78391 | - |
dc.description.abstract | Ulcerative colitis (UC) is characterized by chronic intestinal inflammation as a result of an exaggerated T cell response. Cytotoxic T lymphocyte associated antigen-4 (CTLA-4), expressed mainly in activated T cells, inhibits T cell activation and proliferation by combining B7 through competing CD28 and maintains immune homeostasis. Polymorphisms of the CTLA-4 gene are known to be associated with several autoimmune diseases. The aim of this study was to investigate the association between the CTLA-4 gene micro satellite polymorphism and UC in Chinese patients. Unrelated 100 Chinese patients with UC and 140 healthy controls were studied. The (AT) repeats in the 3′ untranslated region of exon 4 of the CTLA-4 gene were amplified by allele-specific polymerase chain reaction (PCR). The amplified products were electrophoresed on a 12% polyacrylamide gel, followed by silver staining. Twenty alleles were found in Chinese patients and healthy controls. The 122-bp allele was increased in UC compared with healthy controls (9.5% vs 0.7%, P = 0.0001/Pc = 0.002, OR = 14.591, 95% CI 3.357-63.420). The frequency of the longer alleles (≥118 bp) of UC was higher than that in healthy controls (26% vs 4%, P = 0.0001/Pc = 0.0002, OR = 7.644, 95%CI 3.950-14.792), but was not associated with location and severity of the disease. Furthermore, the longer alleles were not associated with haplotypes of C-318T/A+49G of the CTLA-4 gene in Chinese patients with UC. The longer alleles of the CTLA-4 gene microsatellite polymorphism were strongly associated with UC in Chinese patients. Copyright © 2006 by Lippincott Williams & Wilkins. | en_HK |
dc.language | eng | en_HK |
dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.ibdjournal.com | en_HK |
dc.relation.ispartof | Inflammatory Bowel Diseases | en_HK |
dc.subject | Chinese | - |
dc.subject | CTLA-4 | - |
dc.subject | Genetic polymorphism | - |
dc.subject | Ulcerative colitis | - |
dc.subject.mesh | Adolescent | en_HK |
dc.subject.mesh | Adult | en_HK |
dc.subject.mesh | Aged | en_HK |
dc.subject.mesh | Antigens, CD | en_HK |
dc.subject.mesh | Antigens, Differentiation - genetics | en_HK |
dc.subject.mesh | Asian Continental Ancestry Group | en_HK |
dc.subject.mesh | CTLA-4 Antigen | en_HK |
dc.subject.mesh | China - epidemiology | en_HK |
dc.subject.mesh | Colitis, Ulcerative - genetics | en_HK |
dc.subject.mesh | Female | en_HK |
dc.subject.mesh | Genetic Predisposition to Disease | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Male | en_HK |
dc.subject.mesh | Microsatellite Repeats - genetics | en_HK |
dc.subject.mesh | Middle Aged | en_HK |
dc.subject.mesh | Polymorphism, Genetic | en_HK |
dc.title | Association of CTLA-4 gene microsatellite polymorphism with ulcerative colitis in Chinese patients | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Wong, BCY:bcywong@hku.hk | en_HK |
dc.identifier.authority | Wong, BCY=rp00429 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1097/01.MIB.0000217339.61183.dd | en_HK |
dc.identifier.pmid | 16670525 | en_HK |
dc.identifier.scopus | eid_2-s2.0-33646817094 | en_HK |
dc.identifier.hkuros | 117718 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33646817094&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 12 | en_HK |
dc.identifier.issue | 5 | en_HK |
dc.identifier.spage | 369 | en_HK |
dc.identifier.epage | 373 | en_HK |
dc.identifier.isi | WOS:000237370300006 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Jiang, Y=13608424700 | en_HK |
dc.identifier.scopusauthorid | Xia, B=7102762263 | en_HK |
dc.identifier.scopusauthorid | Jiang, L=36077340600 | en_HK |
dc.identifier.scopusauthorid | Lv, M=36728902100 | en_HK |
dc.identifier.scopusauthorid | Guo, Q=12792639800 | en_HK |
dc.identifier.scopusauthorid | Chen, M=35080134300 | en_HK |
dc.identifier.scopusauthorid | Li, J=36065114100 | en_HK |
dc.identifier.scopusauthorid | Xia, HHX=8757161400 | en_HK |
dc.identifier.scopusauthorid | Wong, BCY=7402023340 | en_HK |
dc.identifier.issnl | 1078-0998 | - |