File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Globular and full-length forms of adiponectin mediate specific changes in glucose and fatty acid uptake and metabolism in cardiomyocytes

TitleGlobular and full-length forms of adiponectin mediate specific changes in glucose and fatty acid uptake and metabolism in cardiomyocytes
Authors
KeywordsAdiponectin
Diabetes
Glucose and fatty acid uptake and metabolism
Obesity
Issue Date2007
PublisherOxford University Press. The Journal's web site is located at http://cardiovascres.oxfordjournals.org
Citation
Cardiovascular Research, 2007, v. 75 n. 1, p. 148-157 How to Cite?
AbstractObjective: Our aim was to investigate the regulation of glucose and fatty acid metabolism in cardiomyocytes by the globular (gAd) and full-length (fAd) forms of adiponectin. Methods: We produced fAd (consisting of high, medium and low molecular weight oligomers) in a mammalian expression system and gAd in bacteria. These were used to treat primary neonatal rat cardiomyocytes (up to 48 h), and we employed 3H- or 14C-labeled substrates to monitor glucose uptake and subsequent metabolism via oxidation, glycogen synthesis or lactate production and fatty acid uptake and oxidation. Enzymatic assay for acetyl CoA carboxylase activity was employed, and protein phosphorylation and expression was determined by immunoblotting cell lysates. The role of adiponectin receptor (AdipoR) isoforms was determined via siRNA-mediated knockdown. Results: There was an initial (1 h) increase in glucose uptake and oxidation in response to gAd or fAd. Fatty acid uptake was stimulated by gAd or fAd, and by 24 h a decrease in acetyl CoA carboxylase activity and elevated fatty acid oxidation were observed. After 48 h increased fatty acid oxidation correlated with decreased glucose oxidation and pyruvate dehydrogenase activity, while glycogen synthesis and lactate production increased. Both gAd and fAd elicited phosphorylation of AMP kinase, insulin receptor substrate-1, Akt and glycogen synthase kinase-3β. Knockdown of AdipoR1 or AdipoR2 attenuated the effect of both gAd and fAd on fatty acid uptake and oxidation. Only AdipoR1 knockdown prevented the ability of gAd (1 h) to increase glucose uptake and oxidation; however, reducing either AdipoR1 or AdipoR2 expression attenuated the long-term (24 h) effects of gAd. Conclusions: These results clearly demonstrate that gAd and fAd mediate distinct and time-dependent effects on cardiomyocyte energy metabolism via AdipoR1 and AdipoR2. © 2007 European Society of Cardiology.
Persistent Identifierhttp://hdl.handle.net/10722/76843
ISSN
2021 Impact Factor: 13.081
2020 SCImago Journal Rankings: 2.774
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPalanivel, Ren_HK
dc.contributor.authorFang, Xen_HK
dc.contributor.authorPark, Men_HK
dc.contributor.authorEguchi, Men_HK
dc.contributor.authorPallan, Sen_HK
dc.contributor.authorDe Girolamo, Sen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorXu, Aen_HK
dc.contributor.authorSweeney, Gen_HK
dc.date.accessioned2010-09-06T07:25:31Z-
dc.date.available2010-09-06T07:25:31Z-
dc.date.issued2007en_HK
dc.identifier.citationCardiovascular Research, 2007, v. 75 n. 1, p. 148-157en_HK
dc.identifier.issn0008-6363en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76843-
dc.description.abstractObjective: Our aim was to investigate the regulation of glucose and fatty acid metabolism in cardiomyocytes by the globular (gAd) and full-length (fAd) forms of adiponectin. Methods: We produced fAd (consisting of high, medium and low molecular weight oligomers) in a mammalian expression system and gAd in bacteria. These were used to treat primary neonatal rat cardiomyocytes (up to 48 h), and we employed 3H- or 14C-labeled substrates to monitor glucose uptake and subsequent metabolism via oxidation, glycogen synthesis or lactate production and fatty acid uptake and oxidation. Enzymatic assay for acetyl CoA carboxylase activity was employed, and protein phosphorylation and expression was determined by immunoblotting cell lysates. The role of adiponectin receptor (AdipoR) isoforms was determined via siRNA-mediated knockdown. Results: There was an initial (1 h) increase in glucose uptake and oxidation in response to gAd or fAd. Fatty acid uptake was stimulated by gAd or fAd, and by 24 h a decrease in acetyl CoA carboxylase activity and elevated fatty acid oxidation were observed. After 48 h increased fatty acid oxidation correlated with decreased glucose oxidation and pyruvate dehydrogenase activity, while glycogen synthesis and lactate production increased. Both gAd and fAd elicited phosphorylation of AMP kinase, insulin receptor substrate-1, Akt and glycogen synthase kinase-3β. Knockdown of AdipoR1 or AdipoR2 attenuated the effect of both gAd and fAd on fatty acid uptake and oxidation. Only AdipoR1 knockdown prevented the ability of gAd (1 h) to increase glucose uptake and oxidation; however, reducing either AdipoR1 or AdipoR2 expression attenuated the long-term (24 h) effects of gAd. Conclusions: These results clearly demonstrate that gAd and fAd mediate distinct and time-dependent effects on cardiomyocyte energy metabolism via AdipoR1 and AdipoR2. © 2007 European Society of Cardiology.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://cardiovascres.oxfordjournals.orgen_HK
dc.relation.ispartofCardiovascular Researchen_HK
dc.rightsCardiovascular Research. Copyright © Elsevier BV.en_HK
dc.subjectAdiponectinen_HK
dc.subjectDiabetesen_HK
dc.subjectGlucose and fatty acid uptake and metabolismen_HK
dc.subjectObesityen_HK
dc.subject.meshAdiponectin - metabolism - pharmacology-
dc.subject.meshGlucose - metabolism-
dc.subject.meshMyocytes, Cardiac - metabolism-
dc.subject.meshPalmitates - metabolism-
dc.subject.meshAnimals-
dc.titleGlobular and full-length forms of adiponectin mediate specific changes in glucose and fatty acid uptake and metabolism in cardiomyocytesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0008-6363&volume=75&spage=148&epage=57&date=2007&atitle=Globular+and+full-length+forms+of+adiponectin+mediate+specific+changes+in+glucose+and+fatty+acid+uptake+and+metabolism+in+cardiomyocytes.en_HK
dc.identifier.emailWang, Y: yuwanghk@hku.hken_HK
dc.identifier.emailXu, A: amxu@hkucc.hku.hken_HK
dc.identifier.authorityWang, Y=rp00239en_HK
dc.identifier.authorityXu, A=rp00485en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.cardiores.2007.04.011en_HK
dc.identifier.pmid17499232-
dc.identifier.scopuseid_2-s2.0-34249869321en_HK
dc.identifier.hkuros129100en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34249869321&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume75en_HK
dc.identifier.issue1en_HK
dc.identifier.spage148en_HK
dc.identifier.epage157en_HK
dc.identifier.isiWOS:000247719800019-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridPalanivel, R=8655817100en_HK
dc.identifier.scopusauthoridFang, X=10642149900en_HK
dc.identifier.scopusauthoridPark, M=16426070700en_HK
dc.identifier.scopusauthoridEguchi, M=14031608700en_HK
dc.identifier.scopusauthoridPallan, S=6505544388en_HK
dc.identifier.scopusauthoridDe Girolamo, S=11939682200en_HK
dc.identifier.scopusauthoridLiu, Y=26643544200en_HK
dc.identifier.scopusauthoridWang, Y=34973733700en_HK
dc.identifier.scopusauthoridXu, A=7202655409en_HK
dc.identifier.scopusauthoridSweeney, G=7102852659en_HK
dc.identifier.issnl0008-6363-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats