File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Four-dimensional imaging and quantification of gene expression in early developing zebrafish (Danio rerio) embryos

TitleFour-dimensional imaging and quantification of gene expression in early developing zebrafish (Danio rerio) embryos
Authors
KeywordsCadmium
Four-dimensional imaging
Green fluorescence protein
Sonic hedgehog
Zebrafish embryo
Issue Date2006
PublisherOxford University Press. The Journal's web site is located at http://toxsci.oxfordjournals.org/
Citation
Toxicological Sciences, 2006, v. 90 n. 2, p. 529-538 How to Cite?
AbstractFour-dimensional (4D) imaging is a powerful tool for studying three-dimensional (3D) changes in an organism through time. Different imaging systems for obtaining 3D data from in vivo specimens have been developed but usually involved large and expensive machines. We successfully used a simple inverted compound microscope and a commercially available program to study and quantify in vivo changes in sonic hedgehog (shh expression during early development in a green fluorescence protein (GFP) transgenic zebrafish (Danio rerio) line. We applied the 4D system to study the effect of 100 μM cadmium exposure on shh expression. In control zebrafish embryos, shh:GFP expression was detected at about 9 h post-fertilization (hpf) and increased steadily in the next 7 h, peaking at about 17 hpf and decreasing in the following 4 h. In the same time period, different shh expression volumes were observed in cadmium-treated and control embryos. Embryos affected by cadmium-exposure demonstrated a down-regulation in shh expression. The number of GFP-expressing cells measured by flow cytometry decreased, and expression of neurogenin-1, a downstream target of the shh signaling pathway, was down-regulated, providing additional supporting data on the effects of cadmium on shh. In summary, we demonstrated the setup of a 4D imaging system and its application to the quantification of gene expression. © 2006 Oxford University Press.
Persistent Identifierhttp://hdl.handle.net/10722/76039
ISSN
2023 Impact Factor: 3.4
2023 SCImago Journal Rankings: 0.911
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYu, RMKen_HK
dc.contributor.authorLin, CCen_HK
dc.contributor.authorChan, PKen_HK
dc.contributor.authorChow, ESHen_HK
dc.contributor.authorMurphy, MBen_HK
dc.contributor.authorChan, BPen_HK
dc.contributor.authorMüller, Fen_HK
dc.contributor.authorSträhle, Uen_HK
dc.contributor.authorCheng, SHen_HK
dc.date.accessioned2010-09-06T07:17:01Z-
dc.date.available2010-09-06T07:17:01Z-
dc.date.issued2006en_HK
dc.identifier.citationToxicological Sciences, 2006, v. 90 n. 2, p. 529-538en_HK
dc.identifier.issn1096-6080en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76039-
dc.description.abstractFour-dimensional (4D) imaging is a powerful tool for studying three-dimensional (3D) changes in an organism through time. Different imaging systems for obtaining 3D data from in vivo specimens have been developed but usually involved large and expensive machines. We successfully used a simple inverted compound microscope and a commercially available program to study and quantify in vivo changes in sonic hedgehog (shh expression during early development in a green fluorescence protein (GFP) transgenic zebrafish (Danio rerio) line. We applied the 4D system to study the effect of 100 μM cadmium exposure on shh expression. In control zebrafish embryos, shh:GFP expression was detected at about 9 h post-fertilization (hpf) and increased steadily in the next 7 h, peaking at about 17 hpf and decreasing in the following 4 h. In the same time period, different shh expression volumes were observed in cadmium-treated and control embryos. Embryos affected by cadmium-exposure demonstrated a down-regulation in shh expression. The number of GFP-expressing cells measured by flow cytometry decreased, and expression of neurogenin-1, a downstream target of the shh signaling pathway, was down-regulated, providing additional supporting data on the effects of cadmium on shh. In summary, we demonstrated the setup of a 4D imaging system and its application to the quantification of gene expression. © 2006 Oxford University Press.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://toxsci.oxfordjournals.org/en_HK
dc.relation.ispartofToxicological Sciencesen_HK
dc.rightsToxicological Sciences. Copyright © Oxford University Press.en_HK
dc.subjectCadmium-
dc.subjectFour-dimensional imaging-
dc.subjectGreen fluorescence protein-
dc.subjectSonic hedgehog-
dc.subjectZebrafish embryo-
dc.subject.meshAnimalsen_HK
dc.subject.meshAnimals, Genetically Modifieden_HK
dc.subject.meshBasic Helix-Loop-Helix Transcription Factors - genetics - metabolismen_HK
dc.subject.meshCadmium - toxicityen_HK
dc.subject.meshEmbryo, Nonmammalian - drug effectsen_HK
dc.subject.meshEmbryonic Development - drug effectsen_HK
dc.subject.meshGene Expression Regulation, Developmental - drug effectsen_HK
dc.subject.meshGreen Fluorescent Proteinsen_HK
dc.subject.meshHedgehog Proteinsen_HK
dc.subject.meshMicroscopy, Fluorescence - methodsen_HK
dc.subject.meshNerve Tissue Proteins - genetics - metabolismen_HK
dc.subject.meshTrans-Activators - genetics - metabolismen_HK
dc.subject.meshZebrafish - embryology - genetics - metabolismen_HK
dc.subject.meshZebrafish Proteins - genetics - metabolismen_HK
dc.titleFour-dimensional imaging and quantification of gene expression in early developing zebrafish (Danio rerio) embryosen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1096-6080&volume=90&issue=2&spage=529&epage=538.&date=2006&atitle=Four-dimensional+imaging+and+quantification+of+gene+expression+in+early+developing+zebrafish+(Danio+rerio)+embryosen_HK
dc.identifier.emailChan, BP:bpchan@hkucc.hku.hken_HK
dc.identifier.authorityChan, BP=rp00087en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/toxsci/kfj115en_HK
dc.identifier.pmid16434501-
dc.identifier.scopuseid_2-s2.0-33645115353en_HK
dc.identifier.hkuros118378en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33645115353&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume90en_HK
dc.identifier.issue2en_HK
dc.identifier.spage529en_HK
dc.identifier.epage538en_HK
dc.identifier.isiWOS:000236106000027-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYu, RMK=9278574900en_HK
dc.identifier.scopusauthoridLin, CC=12789042300en_HK
dc.identifier.scopusauthoridChan, PK=7403498052en_HK
dc.identifier.scopusauthoridChow, ESH=35083313200en_HK
dc.identifier.scopusauthoridMurphy, MB=7403900446en_HK
dc.identifier.scopusauthoridChan, BP=7201530390en_HK
dc.identifier.scopusauthoridMüller, F=7402078368en_HK
dc.identifier.scopusauthoridSträhle, U=7004805378en_HK
dc.identifier.scopusauthoridCheng, SH=7404684691en_HK
dc.identifier.citeulike554176-
dc.identifier.issnl1096-0929-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats