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Article: Characterization of microbial community in granular sludge treating brewery wastewater

TitleCharacterization of microbial community in granular sludge treating brewery wastewater
Authors
Keywords16S rRNA
Anaerobic biogranule
Fluorescence in-situ hybridization
Microbial community
Oligonucleotide probes
Issue Date2002
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/watres
Citation
Water Research, 2002, v. 36 n. 7, p. 1767-1775 How to Cite?
AbstractThe diversity and distribution of microbes within brewery-degrading anaerobic sludge granules were studied using various molecular techniques. Molecular cloning of small-subunit rRNA gene sequences indicated that all archaeal clones were affiliated with Methanosaeta concillii (>99% sequence similarity), and the bacterial clones were mostly affiliated with a not-yet-cultured Clostridium cluster (48 out of 99 clones) in the low G+C gram-positive group, Xanthomonas spp. in the γ-subclass of Proteobacteria (30 clones), and Desulfovibrio spp. (16 clones) in the δ-subclass of Proteobacteria. Slot-blot hybridization indicated that archaeal cells from the Methanomicrobiales (58.4% of total rRNA), Methanobacterials (3.3%) and Methanococcales (1.0%) accounted for 62.4% of the total community rRNA. The rest of the microbial populations were the clostridial cluster (27.3% of total rRNA) and Desulfovibrio spp. (9.4%). Fluorescence in-situ hybridization with domain and group-specific oligonucleotide probes further revealed a multi-layer granular architecture. On the surface layer, the hydrolytic clostridial species and hydrogenotrophic Methanobacteriales were the predominant. In the middle layer, mostly H2-producing acetogens from the δ-Proteobacteria (i.e., Desulfovibrio spp.), hydrogenotrophic Methanobacteriales and aceticlastic Methanosaeta were observed to presumably form a syntrophic association. Finally, the center core consisted of microcolonies of Methanosaeta cells. Copyright © 2002 Elsevier Science Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/71126
ISSN
2023 Impact Factor: 11.4
2023 SCImago Journal Rankings: 3.596
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, WTen_HK
dc.contributor.authorChan, OCen_HK
dc.contributor.authorFang, HHPen_HK
dc.date.accessioned2010-09-06T06:29:08Z-
dc.date.available2010-09-06T06:29:08Z-
dc.date.issued2002en_HK
dc.identifier.citationWater Research, 2002, v. 36 n. 7, p. 1767-1775en_HK
dc.identifier.issn0043-1354en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71126-
dc.description.abstractThe diversity and distribution of microbes within brewery-degrading anaerobic sludge granules were studied using various molecular techniques. Molecular cloning of small-subunit rRNA gene sequences indicated that all archaeal clones were affiliated with Methanosaeta concillii (>99% sequence similarity), and the bacterial clones were mostly affiliated with a not-yet-cultured Clostridium cluster (48 out of 99 clones) in the low G+C gram-positive group, Xanthomonas spp. in the γ-subclass of Proteobacteria (30 clones), and Desulfovibrio spp. (16 clones) in the δ-subclass of Proteobacteria. Slot-blot hybridization indicated that archaeal cells from the Methanomicrobiales (58.4% of total rRNA), Methanobacterials (3.3%) and Methanococcales (1.0%) accounted for 62.4% of the total community rRNA. The rest of the microbial populations were the clostridial cluster (27.3% of total rRNA) and Desulfovibrio spp. (9.4%). Fluorescence in-situ hybridization with domain and group-specific oligonucleotide probes further revealed a multi-layer granular architecture. On the surface layer, the hydrolytic clostridial species and hydrogenotrophic Methanobacteriales were the predominant. In the middle layer, mostly H2-producing acetogens from the δ-Proteobacteria (i.e., Desulfovibrio spp.), hydrogenotrophic Methanobacteriales and aceticlastic Methanosaeta were observed to presumably form a syntrophic association. Finally, the center core consisted of microcolonies of Methanosaeta cells. Copyright © 2002 Elsevier Science Ltd.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/watresen_HK
dc.relation.ispartofWater Researchen_HK
dc.subject16S rRNA-
dc.subjectAnaerobic biogranule-
dc.subjectFluorescence in-situ hybridization-
dc.subjectMicrobial community-
dc.subjectOligonucleotide probes-
dc.subject.meshArchaea - classification - genetics - isolation & purificationen_HK
dc.subject.meshClostridium - classification - genetics - isolation & purificationen_HK
dc.subject.meshFood Industryen_HK
dc.subject.meshIn Situ Hybridization, Fluorescenceen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshProteobacteria - classification - genetics - isolation & purificationen_HK
dc.subject.meshRNA, Ribosomal - geneticsen_HK
dc.subject.meshSewage - microbiologyen_HK
dc.subject.meshWater Microbiologyen_HK
dc.subject.meshWater Purificationen_HK
dc.titleCharacterization of microbial community in granular sludge treating brewery wastewateren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0043-1354&volume=36 &issue=7&spage=1767 &epage= 1775&date=2002&atitle=Characterization+of+microbial+community+in+granular+sludge+treating+brewery+wastewateren_HK
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0043-1354(01)00377-3en_HK
dc.identifier.pmid12044076-
dc.identifier.scopuseid_2-s2.0-0036216418en_HK
dc.identifier.hkuros67254en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036216418&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1767en_HK
dc.identifier.epage1775en_HK
dc.identifier.isiWOS:000175807400013-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLiu, WT=37045931100en_HK
dc.identifier.scopusauthoridChan, OC=7004275779en_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK
dc.identifier.issnl0043-1354-

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