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Article: 16S rDNA clone library screening of environmental sample using melting curve analysis
Title | 16S rDNA clone library screening of environmental sample using melting curve analysis |
---|---|
Authors | |
Keywords | 16S rDNA Clone library Melting curve analysis |
Issue Date | 2005 |
Publisher | Taiwan. |
Citation | Journal Of The Chinese Institute Of Engineers, Transactions Of The Chinese Institute Of Engineers,Series A/Chung-Kuo Kung Ch'eng Hsuch K'an, 2005, v. 28 n. 7, p. 1153-1155 How to Cite? |
Abstract | A new method of screening a 16S rDNA clone library from an environmental sample is demonstrated using the non-specific fluorescent dye SYBR Green for the melting curve analysis of PCR-amplified DNA fragments. It is simple, easy and swift to operate. In addition, it requires little manual operation and the product may be still used for further analysis. |
Persistent Identifier | http://hdl.handle.net/10722/70827 |
ISSN | 2023 Impact Factor: 1.0 2023 SCImago Journal Rankings: 0.291 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zhang, T | en_HK |
dc.contributor.author | Fang, HHP | en_HK |
dc.date.accessioned | 2010-09-06T06:26:28Z | - |
dc.date.available | 2010-09-06T06:26:28Z | - |
dc.date.issued | 2005 | en_HK |
dc.identifier.citation | Journal Of The Chinese Institute Of Engineers, Transactions Of The Chinese Institute Of Engineers,Series A/Chung-Kuo Kung Ch'eng Hsuch K'an, 2005, v. 28 n. 7, p. 1153-1155 | en_HK |
dc.identifier.issn | 0253-3839 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/70827 | - |
dc.description.abstract | A new method of screening a 16S rDNA clone library from an environmental sample is demonstrated using the non-specific fluorescent dye SYBR Green for the melting curve analysis of PCR-amplified DNA fragments. It is simple, easy and swift to operate. In addition, it requires little manual operation and the product may be still used for further analysis. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Taiwan. | en_HK |
dc.relation.ispartof | Journal of the Chinese Institute of Engineers, Transactions of the Chinese Institute of Engineers,Series A/Chung-kuo Kung Ch'eng Hsuch K'an | en_HK |
dc.subject | 16S rDNA | en_HK |
dc.subject | Clone library | en_HK |
dc.subject | Melting curve analysis | en_HK |
dc.title | 16S rDNA clone library screening of environmental sample using melting curve analysis | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Zhang, T:zhangt@hkucc.hku.hk | en_HK |
dc.identifier.email | Fang, HHP:hrechef@hkucc.hku.hk | en_HK |
dc.identifier.authority | Zhang, T=rp00211 | en_HK |
dc.identifier.authority | Fang, HHP=rp00115 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.scopus | eid_2-s2.0-28744434906 | en_HK |
dc.identifier.hkuros | 104524 | en_HK |
dc.identifier.hkuros | 120538 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-28744434906&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 28 | en_HK |
dc.identifier.issue | 7 | en_HK |
dc.identifier.spage | 1153 | en_HK |
dc.identifier.epage | 1155 | en_HK |
dc.identifier.isi | WOS:000233645900013 | - |
dc.identifier.scopusauthorid | Zhang, T=24470677400 | en_HK |
dc.identifier.scopusauthorid | Fang, HHP=7402542625 | en_HK |
dc.identifier.issnl | 0253-3839 | - |