File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1111/j.1365-2672.2008.04003.x
- Scopus: eid_2-s2.0-58249114976
- PMID: 19120614
- WOS: WOS:000262475600031
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Microbial characterization and quantification of an anaerobic sludge degrading dimethyl phthalate
Title | Microbial characterization and quantification of an anaerobic sludge degrading dimethyl phthalate | ||||
---|---|---|---|---|---|
Authors | |||||
Keywords | 16S rRNA gene Anaerobic degradation Dimethyl phthalate Microbial analysis UASB | ||||
Issue Date | 2009 | ||||
Publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JAM | ||||
Citation | Journal Of Applied Microbiology, 2009, v. 106 n. 1, p. 296-305 How to Cite? | ||||
Abstract | Aims: Characterization and quantification of microbial community in dimethyl phthalate (DMP)-degrading anaerobic sludge using molecular techniques. Methods and Results: An enriched anaerobic sludge effectively degrading over 99% of dimethyl phthalate in an upflow anaerobic sludge blanket (UASB) reactor for 530 days was characterized and quantified by 16S rRNA-based molecular methods. A total of 78 Bacteria clones were classified into 22 operational taxonomic units (OTUs) in nine divisions, including Firmicutes, Proteobacteria, Chloroflexi, Thermotogae, Bacteroidetes/Chlorobi, Spirochaetes, Acidobacteria and two candidate divisions. The two most abundant OTUs were likely responsible, respectively, for the de-esterification of DMP and the subsequent phthalate degradation. The outer layer of the granule was dominated by Bacteria; whereas the interior was by Archaea, of which 89 ± 5% were acetoclastic Methanosaetaceae and 11 ± 5% hydrogenotrophic Methanomicrobiales. Conclusions: Twenty-two Bacteria OTUs in DMP-degrading anaerobic sludge distributed in nine divisions. The two most abundant OTUs were likely responsible respectively for the de-esterification of DMP and the subsequent phthalate degradation. Layered granular microstructure of DMP-degrading anaerobic sludge suggested that the rate of DMP de-esterification is faster than its inward diffusion rate. Significance and Impact of the Study: This work is the first study to characterize and quantify the microbial community in the anaerobic phthalic ester degrading sludge from the anaerobic reactor. © 2008 The Authors. | ||||
Persistent Identifier | http://hdl.handle.net/10722/70561 | ||||
ISSN | 2023 Impact Factor: 3.2 2023 SCImago Journal Rankings: 0.764 | ||||
ISI Accession Number ID |
Funding Information: The authors wish to thank the Hong Kong Research Grants Council for the financial support of this study (HKU 7107/03E), and D.W. Liang wishes to thank The University of Hong Kong for the postgraduate studentship. | ||||
References | |||||
Grants |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Liang, DW | en_HK |
dc.contributor.author | Fang, HHP | en_HK |
dc.contributor.author | Zhang, T | en_HK |
dc.date.accessioned | 2010-09-06T06:24:05Z | - |
dc.date.available | 2010-09-06T06:24:05Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | Journal Of Applied Microbiology, 2009, v. 106 n. 1, p. 296-305 | en_HK |
dc.identifier.issn | 1364-5072 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/70561 | - |
dc.description.abstract | Aims: Characterization and quantification of microbial community in dimethyl phthalate (DMP)-degrading anaerobic sludge using molecular techniques. Methods and Results: An enriched anaerobic sludge effectively degrading over 99% of dimethyl phthalate in an upflow anaerobic sludge blanket (UASB) reactor for 530 days was characterized and quantified by 16S rRNA-based molecular methods. A total of 78 Bacteria clones were classified into 22 operational taxonomic units (OTUs) in nine divisions, including Firmicutes, Proteobacteria, Chloroflexi, Thermotogae, Bacteroidetes/Chlorobi, Spirochaetes, Acidobacteria and two candidate divisions. The two most abundant OTUs were likely responsible, respectively, for the de-esterification of DMP and the subsequent phthalate degradation. The outer layer of the granule was dominated by Bacteria; whereas the interior was by Archaea, of which 89 ± 5% were acetoclastic Methanosaetaceae and 11 ± 5% hydrogenotrophic Methanomicrobiales. Conclusions: Twenty-two Bacteria OTUs in DMP-degrading anaerobic sludge distributed in nine divisions. The two most abundant OTUs were likely responsible respectively for the de-esterification of DMP and the subsequent phthalate degradation. Layered granular microstructure of DMP-degrading anaerobic sludge suggested that the rate of DMP de-esterification is faster than its inward diffusion rate. Significance and Impact of the Study: This work is the first study to characterize and quantify the microbial community in the anaerobic phthalic ester degrading sludge from the anaerobic reactor. © 2008 The Authors. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Blackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JAM | en_HK |
dc.relation.ispartof | Journal of Applied Microbiology | en_HK |
dc.rights | Journal of Applied Microbiology. Copyright © Blackwell Publishing Ltd. | en_HK |
dc.subject | 16S rRNA gene | - |
dc.subject | Anaerobic degradation | - |
dc.subject | Dimethyl phthalate | - |
dc.subject | Microbial analysis | - |
dc.subject | UASB | - |
dc.subject.mesh | Archaea - classification - genetics - growth & development - metabolism | en_HK |
dc.subject.mesh | Bacteria, Anaerobic - classification - genetics - growth & development - metabolism | en_HK |
dc.subject.mesh | Bioreactors | en_HK |
dc.subject.mesh | DNA, Archaeal - analysis | en_HK |
dc.subject.mesh | DNA, Bacterial - analysis | en_HK |
dc.subject.mesh | Molecular Sequence Data | en_HK |
dc.subject.mesh | Phthalic Acids - metabolism | en_HK |
dc.subject.mesh | Phylogeny | en_HK |
dc.subject.mesh | Polymerase Chain Reaction - methods | en_HK |
dc.subject.mesh | RNA, Ribosomal, 16S - analysis | en_HK |
dc.subject.mesh | Sewage - microbiology | en_HK |
dc.title | Microbial characterization and quantification of an anaerobic sludge degrading dimethyl phthalate | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1364-5072&volume=106&spage=296&epage=305 &date=2008&atitle=Microbial+characterization+and+quantification+of+an+anaerobic+sludge+degrading+dimethyl+phthalate | en_HK |
dc.identifier.email | Fang, HHP:hrechef@hkucc.hku.hk | en_HK |
dc.identifier.email | Zhang, T:zhangt@hkucc.hku.hk | en_HK |
dc.identifier.authority | Fang, HHP=rp00115 | en_HK |
dc.identifier.authority | Zhang, T=rp00211 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1111/j.1365-2672.2008.04003.x | en_HK |
dc.identifier.pmid | 19120614 | - |
dc.identifier.scopus | eid_2-s2.0-58249114976 | en_HK |
dc.identifier.hkuros | 155502 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-58249114976&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 106 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 296 | en_HK |
dc.identifier.epage | 305 | en_HK |
dc.identifier.isi | WOS:000262475600031 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.relation.project | Biodegradation of endocrine disrupters in landfill leachate | - |
dc.identifier.scopusauthorid | Liang, DW=15835235400 | en_HK |
dc.identifier.scopusauthorid | Fang, HHP=7402542625 | en_HK |
dc.identifier.scopusauthorid | Zhang, T=24470677400 | en_HK |
dc.identifier.issnl | 1364-5072 | - |