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- Publisher Website: 10.1007/s00216-005-0092-8
- Scopus: eid_2-s2.0-27744492505
- PMID: 16231133
- WOS: WOS:000233875200017
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Article: Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection
Title | Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection |
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Authors | |
Keywords | Fluorescence detection High performance liquid chromatography Microalgae Paralytic shellfish poisoning toxins |
Issue Date | 2005 |
Publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm |
Citation | Analytical And Bioanalytical Chemistry, 2005, v. 383 n. 6, p. 1014-1017 How to Cite? |
Abstract | A novel method for the determination of paralytic shellfish poisoning (PSP) toxins using high-performance liquid chromatography with fluorescence detection was developed. The fluorescent derivates of neosaxitoxin (neoSTX), saxitoxin (STX), gonyautoxins 1 and 4 (GTX1+4), and gonyautoxins 2 and 3 (GTX2+3) were separated on a μBondapak NH 2 column (300 mm × 3.9 mm, 10 μm) using water and acetate buffer (pH 6.5) as the mobile phase (1.00 mL min -1) in gradient mode with fluorescence detection at 390 nm (excitation at 330 nm). The linear ranges of neoSTX, STX, GTX1+4 and GTX2+3 were 3.31-331, 0.952-95.2, 3.78-378 and 0.124-12.4 ng mL -1, respectively. The detection limits of neoSTX, STX, GTX1+4 and GTX2+3 were 1.10, 0.32, 1.26 and 0.041 ng mL -1, respectively. The method was successfully applied to the determination of PSP toxins in microalgae. The recoveries ranged from 88±2% to 107±4% and the relative standard deviations were 0.16% to 4.4%. The procedure is also environmentally friendly because no organic solvent is used in the mobile phase. © Springer-Verlag 2005. |
Persistent Identifier | http://hdl.handle.net/10722/68554 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.686 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | He, HZ | en_HK |
dc.contributor.author | Li, HB | en_HK |
dc.contributor.author | Jiang, Y | en_HK |
dc.contributor.author | Chen, F | en_HK |
dc.date.accessioned | 2010-09-06T06:05:38Z | - |
dc.date.available | 2010-09-06T06:05:38Z | - |
dc.date.issued | 2005 | en_HK |
dc.identifier.citation | Analytical And Bioanalytical Chemistry, 2005, v. 383 n. 6, p. 1014-1017 | en_HK |
dc.identifier.issn | 1618-2642 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/68554 | - |
dc.description.abstract | A novel method for the determination of paralytic shellfish poisoning (PSP) toxins using high-performance liquid chromatography with fluorescence detection was developed. The fluorescent derivates of neosaxitoxin (neoSTX), saxitoxin (STX), gonyautoxins 1 and 4 (GTX1+4), and gonyautoxins 2 and 3 (GTX2+3) were separated on a μBondapak NH 2 column (300 mm × 3.9 mm, 10 μm) using water and acetate buffer (pH 6.5) as the mobile phase (1.00 mL min -1) in gradient mode with fluorescence detection at 390 nm (excitation at 330 nm). The linear ranges of neoSTX, STX, GTX1+4 and GTX2+3 were 3.31-331, 0.952-95.2, 3.78-378 and 0.124-12.4 ng mL -1, respectively. The detection limits of neoSTX, STX, GTX1+4 and GTX2+3 were 1.10, 0.32, 1.26 and 0.041 ng mL -1, respectively. The method was successfully applied to the determination of PSP toxins in microalgae. The recoveries ranged from 88±2% to 107±4% and the relative standard deviations were 0.16% to 4.4%. The procedure is also environmentally friendly because no organic solvent is used in the mobile phase. © Springer-Verlag 2005. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00216/index.htm | en_HK |
dc.relation.ispartof | Analytical and Bioanalytical Chemistry | en_HK |
dc.subject | Fluorescence detection | en_HK |
dc.subject | High performance liquid chromatography | en_HK |
dc.subject | Microalgae | en_HK |
dc.subject | Paralytic shellfish poisoning toxins | en_HK |
dc.title | Determination of paralytic shellfish poisoning toxins in cultured microalgae by high-performance liquid chromatography with fluorescence detection | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1618-2642&volume=383&spage=1014&epage=1017&date=2005&atitle=Determination+of+paralytic+shellfish+poisoning+toxins+in+cultured+microalgae+by+high-performance+liquid+chromatography+with+fluorescence+detection | en_HK |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_HK |
dc.identifier.authority | Chen, F=rp00672 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s00216-005-0092-8 | en_HK |
dc.identifier.pmid | 16231133 | - |
dc.identifier.scopus | eid_2-s2.0-27744492505 | en_HK |
dc.identifier.hkuros | 122440 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-27744492505&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 383 | en_HK |
dc.identifier.issue | 6 | en_HK |
dc.identifier.spage | 1014 | en_HK |
dc.identifier.epage | 1017 | en_HK |
dc.identifier.isi | WOS:000233875200017 | - |
dc.publisher.place | Germany | en_HK |
dc.identifier.scopusauthorid | He, HZ=55214936100 | en_HK |
dc.identifier.scopusauthorid | Li, HB=25649944400 | en_HK |
dc.identifier.scopusauthorid | Jiang, Y=24605346600 | en_HK |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_HK |
dc.identifier.issnl | 1618-2642 | - |