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Article: Simultaneous determination of carotenoids and chlorophylls in algae by high performance liquid chromatography
Title | Simultaneous determination of carotenoids and chlorophylls in algae by high performance liquid chromatography 高效液相色譜法測定藻類中的類胡蘿卜素和葉綠素 |
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Authors | |
Keywords | High performance liquid chromatography Carotenoids Chlorophylls Algae |
Issue Date | 1997 |
Publisher | 科学出版社 (The Science Press). The Journal's web site is located at http://www.chrom-china.com/ |
Citation | 色譜 , 1997, v. 15 n. 2, p. 133-135 How to Cite? Chinese Journal of Chromatography, 1997, v. 15 n. 2, p. 133-135 How to Cite? |
Abstract | An isocratic reversed-phase HPLC method for the simultaneous determination of carotenoids and chlorophylls in algae is presented. Both carotenoids and chlorophylls were separated by using a C18 column and a mobile phase consisting of d ichloromethane/acetonitrile/methanol/water (22.5 : 9.5: 67. 5:0.5 ). The flow rate was 1. 0mL/min. These pigments were detected by a UV-VIS absorbance detector set at 450nm. The average recoveries of these pigments by standard addition method were 99. 1% for lutein,98. 5 % for a- carotene, 99. 4% for β- carotene, 100. 6% for chlorophyll a and 99. 9% for chlorophyll b. The relative standard deviations of these pigments were 2. 4% for lutein, 5. 6% for α- carotene, 6.0% for β-carotene, 4. 1 % for chlorophyll a and 4. 0% for chlorophyll b. The calibration curves for these pigments gave good linearity over a wide range of o-50mg/L (r=0.9992-0. 9999), The retention times were 3.0 min for lutein, 4. 7 min for chlorophyll b, 6. 3 min for chlorophyll a, 14. 1 min for α- carotene, and 14. 7 min for β-carotene. Using this method, carotenoids and chlorophylls were determined in Spirulina Platensis, Chlorella vulgaris and Haematococcus lacustris. Chlorella vulgaris and Haematococcus lacustris samples were ground in a B. Braun homogenizer. Spirulina platensis sample was smashed by supersonic wave, Acetone was used as extractant. |
Persistent Identifier | http://hdl.handle.net/10722/68540 |
ISSN | 2023 Impact Factor: 1.2 2023 SCImago Journal Rankings: 0.193 |
DC Field | Value | Language |
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dc.contributor.author | Yuan, JP | en_HK |
dc.contributor.author | Zhang, Y | en_HK |
dc.contributor.author | Shi, XM | en_HK |
dc.contributor.author | Gong, XD | en_HK |
dc.contributor.author | Chen, SF | en_HK |
dc.date.accessioned | 2010-09-06T06:05:31Z | - |
dc.date.available | 2010-09-06T06:05:31Z | - |
dc.date.issued | 1997 | en_HK |
dc.identifier.citation | 色譜 , 1997, v. 15 n. 2, p. 133-135 | en_HK |
dc.identifier.citation | Chinese Journal of Chromatography, 1997, v. 15 n. 2, p. 133-135 | - |
dc.identifier.issn | 1000-8713 | - |
dc.identifier.uri | http://hdl.handle.net/10722/68540 | - |
dc.description.abstract | An isocratic reversed-phase HPLC method for the simultaneous determination of carotenoids and chlorophylls in algae is presented. Both carotenoids and chlorophylls were separated by using a C18 column and a mobile phase consisting of d ichloromethane/acetonitrile/methanol/water (22.5 : 9.5: 67. 5:0.5 ). The flow rate was 1. 0mL/min. These pigments were detected by a UV-VIS absorbance detector set at 450nm. The average recoveries of these pigments by standard addition method were 99. 1% for lutein,98. 5 % for a- carotene, 99. 4% for β- carotene, 100. 6% for chlorophyll a and 99. 9% for chlorophyll b. The relative standard deviations of these pigments were 2. 4% for lutein, 5. 6% for α- carotene, 6.0% for β-carotene, 4. 1 % for chlorophyll a and 4. 0% for chlorophyll b. The calibration curves for these pigments gave good linearity over a wide range of o-50mg/L (r=0.9992-0. 9999), The retention times were 3.0 min for lutein, 4. 7 min for chlorophyll b, 6. 3 min for chlorophyll a, 14. 1 min for α- carotene, and 14. 7 min for β-carotene. Using this method, carotenoids and chlorophylls were determined in Spirulina Platensis, Chlorella vulgaris and Haematococcus lacustris. Chlorella vulgaris and Haematococcus lacustris samples were ground in a B. Braun homogenizer. Spirulina platensis sample was smashed by supersonic wave, Acetone was used as extractant. | - |
dc.language | chi | en_HK |
dc.publisher | 科学出版社 (The Science Press). The Journal's web site is located at http://www.chrom-china.com/ | - |
dc.relation.ispartof | 色譜 | en_HK |
dc.relation.ispartof | Chinese Journal of Chromatography | - |
dc.subject | High performance liquid chromatography | - |
dc.subject | Carotenoids | - |
dc.subject | Chlorophylls | - |
dc.subject | Algae | - |
dc.title | Simultaneous determination of carotenoids and chlorophylls in algae by high performance liquid chromatography | en_HK |
dc.title | 高效液相色譜法測定藻類中的類胡蘿卜素和葉綠素 | - |
dc.type | Article | en_HK |
dc.identifier.email | Chen, SF: sfchen@hkusua.hku.hk | en_HK |
dc.identifier.authority | Chen, SF=rp00672 | en_HK |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.hkuros | 21414 | en_HK |
dc.identifier.volume | 15 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 133 | - |
dc.identifier.epage | 135 | - |
dc.publisher.place | China | - |
dc.identifier.issnl | 1000-8713 | - |