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Article: Chromatographic Separation and Purification of trans-Astaxanthin from the Extracts of Haematococcus pluvialis
Title | Chromatographic Separation and Purification of trans-Astaxanthin from the Extracts of Haematococcus pluvialis |
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Authors | |
Keywords | Astaxanthin Astaxanthin esters Carotenoids Haematococcus pluvialis HPLC Purification |
Issue Date | 1998 |
Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau |
Citation | Journal Of Agricultural And Food Chemistry, 1998, v. 46 n. 8, p. 3371-3375 How to Cite? |
Abstract | A gradient reversed-phase HPLC method was developed for the separation of astaxanthin esters and the isomers of astaxanthin from the unsaponified and saponified pigment extracts of the microalga Haematococcus pluvialis. Four kinds of isomers of astaxanthin and astaxanthin esters, (3S,3′S)-trans-astaxanthin, (3S,3′S)-9-cis-astaxanthin, (3S,3′S)-13-cis-astaxanthin, (3R,3′R)-trans-astaxanthin, and their esters, were separated and identified. A small amount of (3S,3′S)-15-trans-astaxanthin was also detected from the saponified extract. In addition, a chromatographic purification method was established for the preparation of natural trans-astaxanthin from the saponified extract of H. pluvialis. With this method, 3.7 mg of astaxanthin was isolated from 1 g of dry biomass of H. pluvialis. The purified astaxanthin contained approximately 97.7% trans-astaxanthin, 1.1% cis-astaxanthin, and 1.2% impurity. |
Persistent Identifier | http://hdl.handle.net/10722/68407 |
ISSN | 2023 Impact Factor: 5.7 2023 SCImago Journal Rankings: 1.114 |
References |
DC Field | Value | Language |
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dc.contributor.author | Yuan, JP | en_HK |
dc.contributor.author | Chen, F | en_HK |
dc.date.accessioned | 2010-09-06T06:04:21Z | - |
dc.date.available | 2010-09-06T06:04:21Z | - |
dc.date.issued | 1998 | en_HK |
dc.identifier.citation | Journal Of Agricultural And Food Chemistry, 1998, v. 46 n. 8, p. 3371-3375 | en_HK |
dc.identifier.issn | 0021-8561 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/68407 | - |
dc.description.abstract | A gradient reversed-phase HPLC method was developed for the separation of astaxanthin esters and the isomers of astaxanthin from the unsaponified and saponified pigment extracts of the microalga Haematococcus pluvialis. Four kinds of isomers of astaxanthin and astaxanthin esters, (3S,3′S)-trans-astaxanthin, (3S,3′S)-9-cis-astaxanthin, (3S,3′S)-13-cis-astaxanthin, (3R,3′R)-trans-astaxanthin, and their esters, were separated and identified. A small amount of (3S,3′S)-15-trans-astaxanthin was also detected from the saponified extract. In addition, a chromatographic purification method was established for the preparation of natural trans-astaxanthin from the saponified extract of H. pluvialis. With this method, 3.7 mg of astaxanthin was isolated from 1 g of dry biomass of H. pluvialis. The purified astaxanthin contained approximately 97.7% trans-astaxanthin, 1.1% cis-astaxanthin, and 1.2% impurity. | en_HK |
dc.language | eng | en_HK |
dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/jafcau | en_HK |
dc.relation.ispartof | Journal of Agricultural and Food Chemistry | en_HK |
dc.subject | Astaxanthin | en_HK |
dc.subject | Astaxanthin esters | en_HK |
dc.subject | Carotenoids | en_HK |
dc.subject | Haematococcus pluvialis | en_HK |
dc.subject | HPLC | en_HK |
dc.subject | Purification | en_HK |
dc.title | Chromatographic Separation and Purification of trans-Astaxanthin from the Extracts of Haematococcus pluvialis | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-8561&volume=46&issue=8&spage=3371&epage=3375&date=1998&atitle=Chromatographic+separation+and+purification+of+trans-astaxanthin+from+the+extracts+of+Haematococcus+pluvialis | en_HK |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_HK |
dc.identifier.authority | Chen, F=rp00672 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.scopus | eid_2-s2.0-0001599028 | en_HK |
dc.identifier.hkuros | 45459 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0001599028&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 46 | en_HK |
dc.identifier.issue | 8 | en_HK |
dc.identifier.spage | 3371 | en_HK |
dc.identifier.epage | 3375 | en_HK |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Yuan, JP=7403401467 | en_HK |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_HK |
dc.identifier.issnl | 0021-8561 | - |