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Article: A multi PDZ-domain protein Pdzd2 contributes to functional expression of sensory neuron-specific sodium channel NaV1.8

TitleA multi PDZ-domain protein Pdzd2 contributes to functional expression of sensory neuron-specific sodium channel NaV1.8
Authors
Shao, DBaker, MDAbrahamsen, BRugiero, FMalikHall, MPoon, WYLCheah, KSEYao, KMWood, JNOkuse, K
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ymcne
Citation
Molecular And Cellular Neuroscience, 2009, v. 42 n. 3, p. 219-225 How to Cite?
DOI: http://dx.doi.org/10.1016/j.mcn.2009.07.003
AbstractThe voltage-gated sodium channel NaV1.8 is expressed exclusively in nociceptive sensory neurons and plays an important role in pain pathways. NaV1.8 cannot be functionally expressed in non-neuronal cells even in the presence of β-subunits. We have previously identified Pdzd2, a multi PDZ-domain protein, as a potential interactor for NaV1.8. Here we report that Pdzd2 binds directly to the intracellular loops of NaV1.8 and NaV1.7. The endogenous NaV1.8 current in sensory neurons is inhibited by antisense- and siRNA-mediated downregulation of Pdzd2. However, no marked change in pain behaviours is observed in Pdzd2-decificent mice. This may be due to compensatory upregulation of p11, another regulatory factor for NaV1.8, in dorsal root ganglia of Pdzd2-deficient mice. These findings reveal that Pdzd2 and p11 play collaborative roles in regulation of NaV1.8 expression in sensory neurons. © 2009 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68025
ISSN
1044-7431
2021 Impact Factor: 4.626
2020 SCImago Journal Rankings: 1.519
PubMed Central ID
PMC2764382
ISI Accession Number ID
WOS:000273497600006
Funding AgencyGrant Number
Wellcome Trust
HKSAR, China
University Grants Committee of the University of HongHKU 7474/04M
AoE/M-04/04
Funding Information:

We thank the Wellcome Trust (DS, MB, WP, JW, KO) and Research Grants Council, HKSAR, China and the University Grants Committee of the University of Hong (KMY: HKU 7474/04M, KC: AoE/M-04/04) for their generous support. We also thank Y. Hata (Tokyo Medical and Dental University) for pCIneo-Papin.

References
References in Scopus
Grants
Investigating the functional involvement of PDZD2 in the regulation of pancreatic beta cell gene expression and function
Developmental genomics and skeletal research

 

DC FieldValueLanguage
dc.contributor.authorShao, Den_HK
dc.contributor.authorBaker, MDen_HK
dc.contributor.authorAbrahamsen, Ben_HK
dc.contributor.authorRugiero, Fen_HK
dc.contributor.authorMalikHall, Men_HK
dc.contributor.authorPoon, WYLen_HK
dc.contributor.authorCheah, KSEen_HK
dc.contributor.authorYao, KMen_HK
dc.contributor.authorWood, JNen_HK
dc.contributor.authorOkuse, Ken_HK
dc.date.accessioned2010-09-06T06:00:37Z-
dc.date.available2010-09-06T06:00:37Z-
dc.date.issued2009en_HK
dc.identifier.citationMolecular And Cellular Neuroscience, 2009, v. 42 n. 3, p. 219-225en_HK
dc.identifier.issn1044-7431en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68025-
dc.description.abstractThe voltage-gated sodium channel NaV1.8 is expressed exclusively in nociceptive sensory neurons and plays an important role in pain pathways. NaV1.8 cannot be functionally expressed in non-neuronal cells even in the presence of β-subunits. We have previously identified Pdzd2, a multi PDZ-domain protein, as a potential interactor for NaV1.8. Here we report that Pdzd2 binds directly to the intracellular loops of NaV1.8 and NaV1.7. The endogenous NaV1.8 current in sensory neurons is inhibited by antisense- and siRNA-mediated downregulation of Pdzd2. However, no marked change in pain behaviours is observed in Pdzd2-decificent mice. This may be due to compensatory upregulation of p11, another regulatory factor for NaV1.8, in dorsal root ganglia of Pdzd2-deficient mice. These findings reveal that Pdzd2 and p11 play collaborative roles in regulation of NaV1.8 expression in sensory neurons. © 2009 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ymcneen_HK
dc.relation.ispartofMolecular and Cellular Neuroscienceen_HK
dc.subject.meshAdaptor Proteins, Signal Transducing - genetics - metabolism-
dc.subject.meshNerve Tissue Proteins - genetics - metabolism-
dc.subject.meshPDZ Domains-
dc.subject.meshSensory Receptor Cells - cytology - metabolism-
dc.subject.meshSodium Channels - genetics - metabolism-
dc.titleA multi PDZ-domain protein Pdzd2 contributes to functional expression of sensory neuron-specific sodium channel NaV1.8en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1044-7431&volume=42&issue=3&spage=219&epage=225&date=2009&atitle=A+multi+PDZ-domain+protein+Pdzd2+contributes+to+functional+expression+of+sensory+neuron-specific+sodium+channel+Nav1.8en_HK
dc.identifier.emailCheah, KSE:hrmbdkc@hku.hken_HK
dc.identifier.emailYao, KM:kmyao@hku.hken_HK
dc.identifier.authorityCheah, KSE=rp00342en_HK
dc.identifier.authorityYao, KM=rp00344en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.mcn.2009.07.003en_HK
dc.identifier.pmid19607921-
dc.identifier.pmcidPMC2764382-
dc.identifier.scopuseid_2-s2.0-69249224518en_HK
dc.identifier.hkuros163791en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-69249224518&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume42en_HK
dc.identifier.issue3en_HK
dc.identifier.spage219en_HK
dc.identifier.epage225en_HK
dc.identifier.isiWOS:000273497600006-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectInvestigating the functional involvement of PDZD2 in the regulation of pancreatic beta cell gene expression and function-
dc.relation.projectDevelopmental genomics and skeletal research-
dc.identifier.scopusauthoridShao, D=7006765448en_HK
dc.identifier.scopusauthoridBaker, MD=34567991000en_HK
dc.identifier.scopusauthoridAbrahamsen, B=7003987415en_HK
dc.identifier.scopusauthoridRugiero, F=12766057300en_HK
dc.identifier.scopusauthoridMalikHall, M=6507366190en_HK
dc.identifier.scopusauthoridPoon, WYL=7103025533en_HK
dc.identifier.scopusauthoridCheah, KSE=35387746200en_HK
dc.identifier.scopusauthoridYao, KM=7403234578en_HK
dc.identifier.scopusauthoridWood, JN=7404353149en_HK
dc.identifier.scopusauthoridOkuse, K=6603069995en_HK
dc.identifier.citeulike5337443-
dc.identifier.issnl1044-7431-

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