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- Publisher Website: 10.1016/S0167-4781(01)00260-3
- Scopus: eid_2-s2.0-0035975112
- PMID: 11513954
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Article: Identification of downstream target genes of latent membrane protein 1 in nasopharyngeal carcinoma cells by suppression subtractive hybridization
Title | Identification of downstream target genes of latent membrane protein 1 in nasopharyngeal carcinoma cells by suppression subtractive hybridization |
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Authors | |
Keywords | Latent membrane protein 1 Nasopharyngeal carcinoma Suppression subtractive hybridization |
Issue Date | 2001 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexp |
Citation | Biochimica Et Biophysica Acta - Gene Structure And Expression, 2001, v. 1520 n. 2, p. 131-140 How to Cite? |
Abstract | Nasopharyngeal carcinoma (NPC) is a common cancer in Southern China and is closely associated with infection of Epstein-Barr virus (EBV). The EBV encoded latent membrane protein 1 (LMP1) is frequently detected in NPC and may play a role in its pathogenesis. Previous studies have shown that LMP1 transformed rodent fibroblasts and altered growth properties in B cells and epithelial cells. However, the pathological role of LMP1 in NPC cells is still poorly understood. In order to investigate the downstream target genes of LMP1 in NPC cells, suppression subtractive hybridization was used to clone and identify the genes differentially expressed in a LMP1 expressing NPC cell line, CNE-2. Two subtractive cDNA libraries were constructed: one enriched for the genes upregulated by LMP1 and one was for the genes downregulated by LMP1. A total of 192 clones were screened by reverse Northern blotting. Fourteen of them were confirmed to be overexpressed while eight of them were suppressed. The upregulation of integrin α6, laminin 5γ2, TAP1 and downregulation of p54nrb, RACK1 and p66Shc were further confirmed in three sets of LMP1 expressing NPC cell lines. The expression profiles of differentially expressed genes identified in this study suggest a role of LMP1 in promotion of cell survival and facilitation of tumor invasion. © 2001 Elsevier Science B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/67960 |
ISSN | |
References |
DC Field | Value | Language |
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dc.contributor.author | Lo, AKF | en_HK |
dc.contributor.author | Liu, Y | en_HK |
dc.contributor.author | Wang, X | en_HK |
dc.contributor.author | Yong Chuan Wong | en_HK |
dc.contributor.author | Lee, CKF | en_HK |
dc.contributor.author | Huang, DP | en_HK |
dc.contributor.author | Sai Wah Tsao | en_HK |
dc.date.accessioned | 2010-09-06T05:59:50Z | - |
dc.date.available | 2010-09-06T05:59:50Z | - |
dc.date.issued | 2001 | en_HK |
dc.identifier.citation | Biochimica Et Biophysica Acta - Gene Structure And Expression, 2001, v. 1520 n. 2, p. 131-140 | en_HK |
dc.identifier.issn | 0167-4781 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/67960 | - |
dc.description.abstract | Nasopharyngeal carcinoma (NPC) is a common cancer in Southern China and is closely associated with infection of Epstein-Barr virus (EBV). The EBV encoded latent membrane protein 1 (LMP1) is frequently detected in NPC and may play a role in its pathogenesis. Previous studies have shown that LMP1 transformed rodent fibroblasts and altered growth properties in B cells and epithelial cells. However, the pathological role of LMP1 in NPC cells is still poorly understood. In order to investigate the downstream target genes of LMP1 in NPC cells, suppression subtractive hybridization was used to clone and identify the genes differentially expressed in a LMP1 expressing NPC cell line, CNE-2. Two subtractive cDNA libraries were constructed: one enriched for the genes upregulated by LMP1 and one was for the genes downregulated by LMP1. A total of 192 clones were screened by reverse Northern blotting. Fourteen of them were confirmed to be overexpressed while eight of them were suppressed. The upregulation of integrin α6, laminin 5γ2, TAP1 and downregulation of p54nrb, RACK1 and p66Shc were further confirmed in three sets of LMP1 expressing NPC cell lines. The expression profiles of differentially expressed genes identified in this study suggest a role of LMP1 in promotion of cell survival and facilitation of tumor invasion. © 2001 Elsevier Science B.V. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbaexp | en_HK |
dc.relation.ispartof | Biochimica et Biophysica Acta - Gene Structure and Expression | en_HK |
dc.rights | Biochimica et Biophysica Acta. Copyright © Elsevier BV. | en_HK |
dc.subject | Latent membrane protein 1 | en_HK |
dc.subject | Nasopharyngeal carcinoma | en_HK |
dc.subject | Suppression subtractive hybridization | en_HK |
dc.subject.mesh | DNA, Complementary - biosynthesis - chemistry | en_HK |
dc.subject.mesh | Gene Library | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Nasopharyngeal Neoplasms - genetics | en_HK |
dc.subject.mesh | Neoplasm Invasiveness | en_HK |
dc.subject.mesh | Neoplasm Proteins - biosynthesis - genetics | en_HK |
dc.subject.mesh | Nucleic Acid Hybridization - methods | en_HK |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_HK |
dc.subject.mesh | Sequence Homology, Nucleic Acid | en_HK |
dc.subject.mesh | Signal Transduction | en_HK |
dc.subject.mesh | Tumor Cells, Cultured | en_HK |
dc.subject.mesh | Viral Matrix Proteins - biosynthesis - genetics | en_HK |
dc.title | Identification of downstream target genes of latent membrane protein 1 in nasopharyngeal carcinoma cells by suppression subtractive hybridization | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3002&volume=1520&spage=131&epage=140&date=2001&atitle=Identification+of+downstream+target+genes+of+latent+membrane+protein+1+in+nasopharyngeal+carcinoma+cells+by+suppression+subtractive+hybridization | en_HK |
dc.identifier.email | Lee, CKF:ckflee@hku.hk | en_HK |
dc.identifier.email | Sai Wah Tsao:gswtsao@hkucc.hku.hk | en_HK |
dc.identifier.authority | Lee, CKF=rp00458 | en_HK |
dc.identifier.authority | Sai Wah Tsao=rp00399 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/S0167-4781(01)00260-3 | en_HK |
dc.identifier.pmid | 11513954 | - |
dc.identifier.scopus | eid_2-s2.0-0035975112 | en_HK |
dc.identifier.hkuros | 64369 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0035975112&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 1520 | en_HK |
dc.identifier.issue | 2 | en_HK |
dc.identifier.spage | 131 | en_HK |
dc.identifier.epage | 140 | en_HK |
dc.publisher.place | Netherlands | en_HK |
dc.identifier.scopusauthorid | Lo, AKF=7102780657 | en_HK |
dc.identifier.scopusauthorid | Liu, Y=26643293600 | en_HK |
dc.identifier.scopusauthorid | Wang, X=23053054900 | en_HK |
dc.identifier.scopusauthorid | Yong Chuan Wong=23053591000 | en_HK |
dc.identifier.scopusauthorid | Lee, CKF=26643097500 | en_HK |
dc.identifier.scopusauthorid | Huang, DP=7403891486 | en_HK |
dc.identifier.scopusauthorid | Sai Wah Tsao=7102813116 | en_HK |
dc.identifier.issnl | 0167-4781 | - |