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- PMID: 16186328
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Article: Transgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons
Title | Transgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons |
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Authors | |
Issue Date | 2005 |
Publisher | Association for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org |
Citation | Investigative Ophthalmology And Visual Science, 2005, v. 46 n. 10, p. 3515-3520 How to Cite? |
Abstract | PURPOSE. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants. METHODS. The IRES-Cre-cDNA. fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity. RESULTS. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKCα antibody (specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina. CONCLUSIONS. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation. Copyright © Association for Research in Vision and Ophthalmology. |
Persistent Identifier | http://hdl.handle.net/10722/67627 |
ISSN | 2023 Impact Factor: 5.0 2023 SCImago Journal Rankings: 1.422 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Zhang, XM | en_HK |
dc.contributor.author | Chen, BY | en_HK |
dc.contributor.author | Ng, AHL | en_HK |
dc.contributor.author | Tanner, JA | en_HK |
dc.contributor.author | Tay, D | en_HK |
dc.contributor.author | So, KF | en_HK |
dc.contributor.author | Rachel, RA | en_HK |
dc.contributor.author | Copeland, NG | en_HK |
dc.contributor.author | Jenkins, NA | en_HK |
dc.contributor.author | Huang, JD | en_HK |
dc.date.accessioned | 2010-09-06T05:56:49Z | - |
dc.date.available | 2010-09-06T05:56:49Z | - |
dc.date.issued | 2005 | en_HK |
dc.identifier.citation | Investigative Ophthalmology And Visual Science, 2005, v. 46 n. 10, p. 3515-3520 | en_HK |
dc.identifier.issn | 0146-0404 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/67627 | - |
dc.description.abstract | PURPOSE. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants. METHODS. The IRES-Cre-cDNA. fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity. RESULTS. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKCα antibody (specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina. CONCLUSIONS. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation. Copyright © Association for Research in Vision and Ophthalmology. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Association for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org | en_HK |
dc.relation.ispartof | Investigative Ophthalmology and Visual Science | en_HK |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Chromosomes, Artificial, Bacterial | en_HK |
dc.subject.mesh | Female | en_HK |
dc.subject.mesh | Fluorescent Antibody Technique, Indirect | en_HK |
dc.subject.mesh | Galactosides - metabolism | en_HK |
dc.subject.mesh | Green Fluorescent Proteins - genetics - metabolism | en_HK |
dc.subject.mesh | Guanine Nucleotide Exchange Factors | en_HK |
dc.subject.mesh | Indoles - metabolism | en_HK |
dc.subject.mesh | Integrases - genetics - metabolism | en_HK |
dc.subject.mesh | Interneurons - enzymology | en_HK |
dc.subject.mesh | Male | en_HK |
dc.subject.mesh | Mice | en_HK |
dc.subject.mesh | Mice, Inbred C3H | en_HK |
dc.subject.mesh | Mice, Inbred C57BL | en_HK |
dc.subject.mesh | Mice, Knockout | en_HK |
dc.subject.mesh | Mice, Transgenic | en_HK |
dc.subject.mesh | Neuropeptides - genetics | en_HK |
dc.subject.mesh | Pregnancy | en_HK |
dc.subject.mesh | Purkinje Cells - enzymology | en_HK |
dc.subject.mesh | Retinal Rod Photoreceptor Cells - cytology - embryology | en_HK |
dc.subject.mesh | beta-Galactosidase - metabolism | en_HK |
dc.title | Transgenic mice expressing Cre-recombinase specifically in retinal rod bipolar neurons | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Tanner, JA:jatanner@hku.hk | en_HK |
dc.identifier.email | Tay, D:dkctay@hkucc.hku.hk | en_HK |
dc.identifier.email | So, KF:hrmaskf@hkucc.hku.hk | en_HK |
dc.identifier.email | Huang, JD:jdhuang@hkucc.hku.hk | en_HK |
dc.identifier.authority | Tanner, JA=rp00495 | en_HK |
dc.identifier.authority | Tay, D=rp00336 | en_HK |
dc.identifier.authority | So, KF=rp00329 | en_HK |
dc.identifier.authority | Huang, JD=rp00451 | en_HK |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.1167/iovs.04-1201 | en_HK |
dc.identifier.pmid | 16186328 | en_HK |
dc.identifier.scopus | eid_2-s2.0-32944457757 | en_HK |
dc.identifier.hkuros | 107078 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-32944457757&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 46 | en_HK |
dc.identifier.issue | 10 | en_HK |
dc.identifier.spage | 3515 | en_HK |
dc.identifier.epage | 3520 | en_HK |
dc.identifier.isi | WOS:000232112900009 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Zhang, XM=7410273116 | en_HK |
dc.identifier.scopusauthorid | Chen, BY=14051424300 | en_HK |
dc.identifier.scopusauthorid | Ng, AHL=12445077000 | en_HK |
dc.identifier.scopusauthorid | Tanner, JA=35513993000 | en_HK |
dc.identifier.scopusauthorid | Tay, D=7006796825 | en_HK |
dc.identifier.scopusauthorid | So, KF=34668391300 | en_HK |
dc.identifier.scopusauthorid | Rachel, RA=7003812116 | en_HK |
dc.identifier.scopusauthorid | Copeland, NG=35374759300 | en_HK |
dc.identifier.scopusauthorid | Jenkins, NA=35379887700 | en_HK |
dc.identifier.scopusauthorid | Huang, JD=8108660600 | en_HK |
dc.identifier.issnl | 0146-0404 | - |