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Article: Effect of leptin on motility, capacitation and acrosome reaction of human spermatozoa

TitleEffect of leptin on motility, capacitation and acrosome reaction of human spermatozoa
Authors
Li, HWRChiu, PCNCheung, MPLYeung, WSBO, WS
KeywordsAcrosome reaction
Capacitation
Leptin
Leptin receptor
Motility
Spermatozoa
Issue Date2009
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IJA
Citation
International Journal Of Andrology, 2009, v. 32 n. 6, p. 687-694 How to Cite?
DOI: http://dx.doi.org/10.1111/j.1365-2605.2008.00931.x
AbstractLeptin is a polypeptide hormone with important roles in reproduction. It has been detected in human seminal plasma as well as on human ejaculated spermatozoa. This study aimed at studying the possible role of leptin in regulating human sperm functions. Immunofluorescent staining was used to study the expression of leptin and its receptor. The correlation between the concentration of leptin and soluble leptin receptor (ObRs) in seminal plasma as measured by enzyme-linked immunosorbant assay and sperm motility parameters measured by computer-assisted sperm analyais (CASA) was determined. The effects of recombinant leptin on human sperm motility, capacitation and acrosome reaction as measured by chlortetracycline staing were also studied. Leptin immunoreactivity was demonstrated at the equatorial and neck regions of human spermatozoa, whereas that of ObRs was shown up on the tail. After Percoll separation, spermatozoa with high density had more intense leptin immunoreactivity compared with those with low density. No significant correlation was found between seminal plasma concentration of leptin/ObRs and sperm motility parameters. After incubation with recombinant human leptin for either 3 h or overnight, there was no change in all the CASA motility parameters determined and percentages of capacitated and acrosome-reacted spermatozoa. We concluded that leptin does not have a significant effect on motility and capacitation/acrosome reaction in human ejaculated mature spermatozoa. Its role in male reproduction is yet to be determined. © 2009 European Academy of Andrology.
Persistent Identifierhttp://hdl.handle.net/10722/67613
ISSN
0105-6263
2014 Impact Factor: 3.695
ISI Accession Number ID
WOS:000271521500012
Funding AgencyGrant Number
Seed Funding Programme for Basic Research
University of Hong Kong
Family Planning Association of Hong Kong
Funding Information:

This study was supported by a research grant from the Seed Funding Programme for Basic Research, The University of Hong Kong. The authors would like to thank Ms Sindy Ling and Mr Chung Man Kin for technical assistance, and Dr Susan Fan, Dr Sue Lo and other staff of the Family Planning Association of Hong Kong for supporting this study and facilitating sample collection.

References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorLi, HWRen_HK
dc.contributor.authorChiu, PCNen_HK
dc.contributor.authorCheung, MPLen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorO, WSen_HK
dc.date.accessioned2010-09-06T05:56:41Z-
dc.date.available2010-09-06T05:56:41Z-
dc.date.issued2009en_HK
dc.identifier.citationInternational Journal Of Andrology, 2009, v. 32 n. 6, p. 687-694en_HK
dc.identifier.issn0105-6263en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67613-
dc.description.abstractLeptin is a polypeptide hormone with important roles in reproduction. It has been detected in human seminal plasma as well as on human ejaculated spermatozoa. This study aimed at studying the possible role of leptin in regulating human sperm functions. Immunofluorescent staining was used to study the expression of leptin and its receptor. The correlation between the concentration of leptin and soluble leptin receptor (ObRs) in seminal plasma as measured by enzyme-linked immunosorbant assay and sperm motility parameters measured by computer-assisted sperm analyais (CASA) was determined. The effects of recombinant leptin on human sperm motility, capacitation and acrosome reaction as measured by chlortetracycline staing were also studied. Leptin immunoreactivity was demonstrated at the equatorial and neck regions of human spermatozoa, whereas that of ObRs was shown up on the tail. After Percoll separation, spermatozoa with high density had more intense leptin immunoreactivity compared with those with low density. No significant correlation was found between seminal plasma concentration of leptin/ObRs and sperm motility parameters. After incubation with recombinant human leptin for either 3 h or overnight, there was no change in all the CASA motility parameters determined and percentages of capacitated and acrosome-reacted spermatozoa. We concluded that leptin does not have a significant effect on motility and capacitation/acrosome reaction in human ejaculated mature spermatozoa. Its role in male reproduction is yet to be determined. © 2009 European Academy of Andrology.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/IJAen_HK
dc.relation.ispartofInternational Journal of Andrologyen_HK
dc.rightsInternational Journal of Andrology. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectAcrosome reactionen_HK
dc.subjectCapacitationen_HK
dc.subjectLeptinen_HK
dc.subjectLeptin receptoren_HK
dc.subjectMotilityen_HK
dc.subjectSpermatozoaen_HK
dc.subject.meshAcrosome Reaction - drug effects - physiologyen_HK
dc.subject.meshCell Counten_HK
dc.subject.meshChlortetracycline - metabolism - pharmacologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLeptin - metabolism - pharmacologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshSemen - metabolismen_HK
dc.subject.meshSperm Capacitation - drug effectsen_HK
dc.subject.meshSperm Motility - drug effects - physiologyen_HK
dc.subject.meshSpermatozoa - drug effects - metabolism - physiologyen_HK
dc.titleEffect of leptin on motility, capacitation and acrosome reaction of human spermatozoaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0105-6263&volume=32&spage=687&epage=694&date=2009&atitle=Effect+of+leptin+on+motility,+capacitation+and+acrosome+reaction+of+human+spermatozoaen_HK
dc.identifier.emailChiu, PCN:pchiucn@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailO, WS:owaisum@hkucc.hku.hken_HK
dc.identifier.authorityChiu, PCN=rp00424en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1365-2605.2008.00931.xen_HK
dc.identifier.pmid19076257-
dc.identifier.scopuseid_2-s2.0-70449572487en_HK
dc.identifier.hkuros168109en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70449572487&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume32en_HK
dc.identifier.issue6en_HK
dc.identifier.spage687en_HK
dc.identifier.epage694en_HK
dc.identifier.isiWOS:000271521500012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLi, HWR=37085860800en_HK
dc.identifier.scopusauthoridChiu, PCN=25959969200en_HK
dc.identifier.scopusauthoridCheung, MPL=16749051200en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK
dc.identifier.citeulike6202541-
dc.identifier.issnl0105-6263-

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