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- Publisher Website: 10.1034/j.1600-0765.2003.00606.x
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- PMID: 12753369
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Article: Granulocyte elastase activity in static and flow gingival crevicular fluid
Title | Granulocyte elastase activity in static and flow gingival crevicular fluid |
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Authors | |
Keywords | Chronic periodontitis Gingival crevicular fluid Gingivitis Granulocyte elastase |
Issue Date | 2003 |
Publisher | Wiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1 |
Citation | Journal Of Periodontal Research, 2003, v. 38 n. 3, p. 303-310 How to Cite? |
Abstract | Objectives: This study aimed to evaluate the volume of gingival crevicular fluid (GCF) and granulocyte elastase activity in static GCF (sGCF) and flow GCF (fGCF) from subjects with various periodontal conditions. Methods: Eleven periodontally healthy, 10 gingivitis and 12 periodontitis subjects were recruited and the sites investigated consisted of healthy sites from healthy subjects (HH); healthy (HG) and gingivitis sites (GG) from gingivitis subjects; and healthy (HP), gingivitis (GP) and periodontitis sites (PP) from periodontitis subjects. fGCF samples were collected either 1 min or 5 min following sGCF collection by paper strip technique. GCF volume was determined by Periotron® 6000 and granulocyte elastase activity was assayed with a specific substrate [L-pyroglutamyl-L-prolyl-L-valine-p-nitroanilide(pGluProVal- pNA)]. Results: At baseline, no significant differences existed in clinical and GCF parameters between the two matched sites for subsequent collection of fGCF samples either 1 min or 5 min after sGCF sampling in all subjects. The flow exudate in HG and HP sites quickly replenished to sGCF levels, while a delayed replenishment was found in HH sites, despite the similar sGCF volumes of these sites. The GCF volume and elastase levels in the fGCF at 1 min were higher in GP sites than in GG sites (P < 0.05). Overall, depletion of elastase levels in the fGCF at 1 min was observed in all subjects, whereas elastase levels in the fGCF at 5 min had replenished to sGCF levels in HP, GP, PP sites and GG sites, but had remained at a lower level in HH and HG sites. An overall positive correlation was found between sGCF and fGCF for GCF volume and elastase activity (P < 0.001); however, this correlation varied with GCF parameters and with site conditions of the subjects concerned. Conclusions: This study shows that patterns of dynamic changes in GCF flow and elastase activity varied under different periodontal conditions. Assessment of both sGCF and fGCF may allow better insight into the dynamic change of the target components in GCF. |
Persistent Identifier | http://hdl.handle.net/10722/66716 |
ISSN | 2023 Impact Factor: 3.4 2023 SCImago Journal Rankings: 0.895 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Jin, L | en_HK |
dc.contributor.author | Yu, C | en_HK |
dc.contributor.author | Corbet, EF | en_HK |
dc.date.accessioned | 2010-09-06T05:48:43Z | - |
dc.date.available | 2010-09-06T05:48:43Z | - |
dc.date.issued | 2003 | en_HK |
dc.identifier.citation | Journal Of Periodontal Research, 2003, v. 38 n. 3, p. 303-310 | en_HK |
dc.identifier.issn | 0022-3484 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/66716 | - |
dc.description.abstract | Objectives: This study aimed to evaluate the volume of gingival crevicular fluid (GCF) and granulocyte elastase activity in static GCF (sGCF) and flow GCF (fGCF) from subjects with various periodontal conditions. Methods: Eleven periodontally healthy, 10 gingivitis and 12 periodontitis subjects were recruited and the sites investigated consisted of healthy sites from healthy subjects (HH); healthy (HG) and gingivitis sites (GG) from gingivitis subjects; and healthy (HP), gingivitis (GP) and periodontitis sites (PP) from periodontitis subjects. fGCF samples were collected either 1 min or 5 min following sGCF collection by paper strip technique. GCF volume was determined by Periotron® 6000 and granulocyte elastase activity was assayed with a specific substrate [L-pyroglutamyl-L-prolyl-L-valine-p-nitroanilide(pGluProVal- pNA)]. Results: At baseline, no significant differences existed in clinical and GCF parameters between the two matched sites for subsequent collection of fGCF samples either 1 min or 5 min after sGCF sampling in all subjects. The flow exudate in HG and HP sites quickly replenished to sGCF levels, while a delayed replenishment was found in HH sites, despite the similar sGCF volumes of these sites. The GCF volume and elastase levels in the fGCF at 1 min were higher in GP sites than in GG sites (P < 0.05). Overall, depletion of elastase levels in the fGCF at 1 min was observed in all subjects, whereas elastase levels in the fGCF at 5 min had replenished to sGCF levels in HP, GP, PP sites and GG sites, but had remained at a lower level in HH and HG sites. An overall positive correlation was found between sGCF and fGCF for GCF volume and elastase activity (P < 0.001); however, this correlation varied with GCF parameters and with site conditions of the subjects concerned. Conclusions: This study shows that patterns of dynamic changes in GCF flow and elastase activity varied under different periodontal conditions. Assessment of both sGCF and fGCF may allow better insight into the dynamic change of the target components in GCF. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Wiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1 | en_HK |
dc.relation.ispartof | Journal of Periodontal Research | en_HK |
dc.subject | Chronic periodontitis | - |
dc.subject | Gingival crevicular fluid | - |
dc.subject | Gingivitis | - |
dc.subject | Granulocyte elastase | - |
dc.subject.mesh | Adult | en_HK |
dc.subject.mesh | Alveolar Bone Loss - enzymology | en_HK |
dc.subject.mesh | Analysis of Variance | en_HK |
dc.subject.mesh | Case-Control Studies | en_HK |
dc.subject.mesh | Chronic Disease | en_HK |
dc.subject.mesh | Dental Plaque Index | en_HK |
dc.subject.mesh | Gingival Crevicular Fluid - enzymology - secretion | en_HK |
dc.subject.mesh | Gingivitis - enzymology | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Leukocyte Elastase - analysis | en_HK |
dc.subject.mesh | Linear Models | en_HK |
dc.subject.mesh | Middle Aged | en_HK |
dc.subject.mesh | Periodontal Attachment Loss - enzymology | en_HK |
dc.subject.mesh | Periodontal Index | en_HK |
dc.subject.mesh | Periodontal Pocket - enzymology | en_HK |
dc.subject.mesh | Periodontitis - enzymology | en_HK |
dc.subject.mesh | Periodontium - enzymology | en_HK |
dc.subject.mesh | Secretory Rate | en_HK |
dc.subject.mesh | Time Factors | en_HK |
dc.title | Granulocyte elastase activity in static and flow gingival crevicular fluid | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3484&volume=38&spage=303&epage=310&date=2003&atitle=Granulocyte+elastase+activity+in+static+and+flow+gingival+crevicular+fluid | en_HK |
dc.identifier.email | Jin, L:ljjin@hkucc.hku.hk | en_HK |
dc.identifier.email | Corbet, EF:efcorbet@hku.hk | en_HK |
dc.identifier.authority | Jin, L=rp00028 | en_HK |
dc.identifier.authority | Corbet, EF=rp00005 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1034/j.1600-0765.2003.00606.x | - |
dc.identifier.pmid | 12753369 | - |
dc.identifier.scopus | eid_2-s2.0-0042760456 | en_HK |
dc.identifier.hkuros | 80314 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0042760456&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 38 | en_HK |
dc.identifier.issue | 3 | en_HK |
dc.identifier.spage | 303 | en_HK |
dc.identifier.epage | 310 | en_HK |
dc.identifier.isi | WOS:000182984800012 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Jin, L=7403328850 | en_HK |
dc.identifier.scopusauthorid | Yu, C=7404977564 | en_HK |
dc.identifier.scopusauthorid | Corbet, EF=35609873200 | en_HK |
dc.identifier.issnl | 0022-3484 | - |