The in vivo expression patterns of IL-1!b and IL-10 in chronic periodontitis

Abstract

Objective To describe and characterize the in vivo expression profile of IL-1β and IL-10 in various categories of human gingival tissues and investigate the correlations between these two cytokines in periodontal health and disease. Methods Gingival biopsies were collected from 24 subjects with chronic periodontitis, including periodontal pocket tissues (PoTs), inflamed connective tissues (ICTs) and clinically healthy gingival tissues (HT-Ps), and from 7 periodontally healthy subjects as controls (HT-Cs). The expression of IL-1β and IL-10 was detected by immunohistochemistry. Results IL-1β mainly expressed on the surface of nuclear membrane of baser layer cells as well as in the cytoplasm of upper cells of epithelium. While IL-10 mainly expressed in the intercellular spaces of connective tissues beneath the gingival epithelium. Within the patients, the expression levels of IL-1β were significantly higher in ICTs compared to those of HT-Ps (Area% and Cell/Area). PoTs also exhibited significantly higher expression of IL-1β than HT-Ps (Cell/Area),while the mean expression levels of IL-10 showed the contrast results. Furthermore, significantly negative correlations were detected between IL-1β levels and IL-10 levels in overall tissues. Conclusions These results suggested that the dynamic interactions between pro- and anti-inflammatory cytokines might be crucial in periodontal pathogenesis. 目的 描述白介素-1β(IL-1β)与白介素-10(IL-10)在人牙龈组织中的表达方式,并研究这两种细胞因子在正常及病理牙周组织中的相互关系。方法 从24例慢性牙周病患者中取得牙周袋组织 (PoTs), 炎性结缔组织 (ICTs) 及临床健康型牙龈组织 (HT-Ps),7例牙周健康组织 (HT-Cs) 作为对照组。结果 IL-1β主要表达在牙龈上皮基底细胞的核膜表面及上层细胞的细胞浆中;而IL-10则主要表达在结缔组织的细胞间隙中。IL-1β在PoT和ICT中的水平比HT-P中显著增多,而IL-10则表现为相反的表达水平。二者呈现出显著的负相关关联。结论 炎性细胞因子和抗炎细胞因子的相互作用对于研究牙周病的病因是很关键的。