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Conference Paper: Cell cycle related kinase (CCRK) is required for centrosome duplication in colon cancer cells
| Title | Cell cycle related kinase (CCRK) is required for centrosome duplication in colon cancer cells |
|---|---|
| Authors | |
| Issue Date | 2008 |
| Publisher | American Society for Cell Biology |
| Citation | 48th Annual Meeting of American Society for Cell Biology, San Francisco, CA, 13-17 December 2008, Abstract no. 410/B357 How to Cite? |
| Abstract | Background & Aims: Cell cycle related kinase (CCRK) is a newly identified cdk-activating kinase (CAK) homologous to Cdk7. The aim of
this study was to investigate the role of CCRK in centrosome duplication in colon cancer cells. Methods: We designed small interfering
RNAs targeting CCRK (siCCRK) and examined cell cycle progression, centrosome duplication, and mitotic catastrophe in colon cancer cell
lines. Results: Suppression of CCRK by siCCRK could significantly inhibit the growth of the colon cancer cells. Flow cytometry analysis
demonstrated that CCRK knock-down resulted in G1 and G2-M phase arrests, induced mitotic catastrophe and suppressed centrosome
duplication, which were accompanied by downregulation in cyclin E, phosphorylated Cdk2, and phosphorylated Rb. Conclusions: CCRK is
crucial for cell cycle progression and is necessary for centrosome duplication in colon cancer cells. |
| Persistent Identifier | http://hdl.handle.net/10722/61678 |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | An, XM | en_HK |
| dc.contributor.author | Ng, SM | en_HK |
| dc.contributor.author | Kung, HF | en_HK |
| dc.contributor.author | Lin, MC | en_HK |
| dc.date.accessioned | 2010-07-13T03:44:56Z | - |
| dc.date.available | 2010-07-13T03:44:56Z | - |
| dc.date.issued | 2008 | en_HK |
| dc.identifier.citation | 48th Annual Meeting of American Society for Cell Biology, San Francisco, CA, 13-17 December 2008, Abstract no. 410/B357 | - |
| dc.identifier.uri | http://hdl.handle.net/10722/61678 | - |
| dc.description.abstract | Background & Aims: Cell cycle related kinase (CCRK) is a newly identified cdk-activating kinase (CAK) homologous to Cdk7. The aim of this study was to investigate the role of CCRK in centrosome duplication in colon cancer cells. Methods: We designed small interfering RNAs targeting CCRK (siCCRK) and examined cell cycle progression, centrosome duplication, and mitotic catastrophe in colon cancer cell lines. Results: Suppression of CCRK by siCCRK could significantly inhibit the growth of the colon cancer cells. Flow cytometry analysis demonstrated that CCRK knock-down resulted in G1 and G2-M phase arrests, induced mitotic catastrophe and suppressed centrosome duplication, which were accompanied by downregulation in cyclin E, phosphorylated Cdk2, and phosphorylated Rb. Conclusions: CCRK is crucial for cell cycle progression and is necessary for centrosome duplication in colon cancer cells. | - |
| dc.language | eng | en_HK |
| dc.publisher | American Society for Cell Biology | - |
| dc.relation.ispartof | Annual Meeting of American Society for Cell Biology | - |
| dc.title | Cell cycle related kinase (CCRK) is required for centrosome duplication in colon cancer cells | en_HK |
| dc.type | Conference_Paper | en_HK |
| dc.identifier.email | Ng, SM: ssmng@hku.hk | en_HK |
| dc.identifier.email | Lin, MC: mcllin@HKUCC.hku.hk | en_HK |
| dc.identifier.authority | Ng, SM=rp00767 | en_HK |
| dc.identifier.authority | Lin, MC=rp00746 | en_HK |
| dc.identifier.hkuros | 152876 | en_HK |