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Article: A Brassica juncea chitinase with two-chitin binding domains show anti-microbial properties against phytopathogens and Gram-negative bacteria
Title | A Brassica juncea chitinase with two-chitin binding domains show anti-microbial properties against phytopathogens and Gram-negative bacteria |
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Authors | |
Keywords | Bacterial agglutination Chitin-binding domain Indian mustard Lectin Phytopathogens Pichia-expressed proteins Transplastomic tobacco |
Issue Date | 2008 |
Publisher | Landes Bioscience. The Journal's web site is located at http://www.landesbioscience.com/journals/psb |
Citation | Plant Signaling And Behavior, 2008, v. 3 n. 12, p. 1103-1105 How to Cite? |
Abstract | In our recent paper in the Journal of Experimental Botany, we demonstrated that Brassica juncea BjCHI1 shows anti-fungal properties against phytopathogens, Colletotrichum truncatum, C. acutatum, Botrytis cinerea and Ascochyta rabiei. Furthermore, BjCHI1 which is an unusual plant chitinase with two (almost identical) chitin-binding domains, agglutinates Gram-negative bacteria, adversely affecting their growth. In contrast, BjCHI1 derivatives lacking one or both domains do not show agglutination activity, suggesting that both chitin-binding domains are essential for agglutination. Observations that agglutination could be relieved by addition of galactose, glucose or lactose, imply that BjCHI1 interacts with the carbohydrate components of the Gram-negative bacterial cell wall. We propose here, a model for BjCHI1-mediated agglutination between Gram-negative bacteria, through interaction of their adjacent cell walls mediated by the two chitin-binding domains of BjCHI1. BjCHI1 is a plant chitinase which has evolved towards acquiring an enhanced role in plant defense against fungi and Gram-negative bacteria. Hence, it is a promising candidate for applications against phytopathogens in plant genetic engineering via nuclear or plastid transformation. ©2008 Landes Bioscience. |
Persistent Identifier | http://hdl.handle.net/10722/60720 |
ISSN | 2023 Impact Factor: 2.8 2023 SCImago Journal Rankings: 0.725 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Guan, Y | en_HK |
dc.contributor.author | Chye, ML | en_HK |
dc.date.accessioned | 2010-05-31T04:17:07Z | - |
dc.date.available | 2010-05-31T04:17:07Z | - |
dc.date.issued | 2008 | en_HK |
dc.identifier.citation | Plant Signaling And Behavior, 2008, v. 3 n. 12, p. 1103-1105 | en_HK |
dc.identifier.issn | 1559-2316 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/60720 | - |
dc.description.abstract | In our recent paper in the Journal of Experimental Botany, we demonstrated that Brassica juncea BjCHI1 shows anti-fungal properties against phytopathogens, Colletotrichum truncatum, C. acutatum, Botrytis cinerea and Ascochyta rabiei. Furthermore, BjCHI1 which is an unusual plant chitinase with two (almost identical) chitin-binding domains, agglutinates Gram-negative bacteria, adversely affecting their growth. In contrast, BjCHI1 derivatives lacking one or both domains do not show agglutination activity, suggesting that both chitin-binding domains are essential for agglutination. Observations that agglutination could be relieved by addition of galactose, glucose or lactose, imply that BjCHI1 interacts with the carbohydrate components of the Gram-negative bacterial cell wall. We propose here, a model for BjCHI1-mediated agglutination between Gram-negative bacteria, through interaction of their adjacent cell walls mediated by the two chitin-binding domains of BjCHI1. BjCHI1 is a plant chitinase which has evolved towards acquiring an enhanced role in plant defense against fungi and Gram-negative bacteria. Hence, it is a promising candidate for applications against phytopathogens in plant genetic engineering via nuclear or plastid transformation. ©2008 Landes Bioscience. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Landes Bioscience. The Journal's web site is located at http://www.landesbioscience.com/journals/psb | en_HK |
dc.relation.ispartof | Plant Signaling and Behavior | en_HK |
dc.subject | Bacterial agglutination | en_HK |
dc.subject | Chitin-binding domain | en_HK |
dc.subject | Indian mustard | en_HK |
dc.subject | Lectin | en_HK |
dc.subject | Phytopathogens | en_HK |
dc.subject | Pichia-expressed proteins | en_HK |
dc.subject | Transplastomic tobacco | en_HK |
dc.title | A Brassica juncea chitinase with two-chitin binding domains show anti-microbial properties against phytopathogens and Gram-negative bacteria | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Chye, ML: mlchye@hkucc.hku.hk | en_HK |
dc.identifier.authority | Chye, ML=rp00687 | en_HK |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.4161/psb.3.12.7006 | - |
dc.identifier.scopus | eid_2-s2.0-57749111535 | en_HK |
dc.identifier.hkuros | 154146 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-57749111535&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 3 | en_HK |
dc.identifier.issue | 12 | en_HK |
dc.identifier.spage | 1103 | en_HK |
dc.identifier.epage | 1105 | en_HK |
dc.identifier.isi | WOS:000213939300015 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Chye, ML=7003905460 | en_HK |
dc.identifier.issnl | 1559-2316 | - |