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- Publisher Website: 10.1210/en.2008-1060
- Scopus: eid_2-s2.0-66449117778
- PMID: 19106223
- WOS: WOS:000265407500032
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Article: The secretogranin II-derived peptide secretoneurin stimulates luteinizing hormone secretion from gonadotrophs
Title | The secretogranin II-derived peptide secretoneurin stimulates luteinizing hormone secretion from gonadotrophs | ||||||||
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Authors | |||||||||
Issue Date | 2009 | ||||||||
Publisher | The Endocrine Society. The Journal's web site is located at http://endo.endojournals.org | ||||||||
Citation | Endocrinology, 2009, v. 150 n. 5, p. 2273-2282 How to Cite? | ||||||||
Abstract | Secretoneurin (SN) isa 33-to34-aminoacid neuropeptide derived from secretogranin-II, a member of the chromogranin family. We previously synthesized a putative goldfish (gf) SN and demonstrated its ability to stimulate LH release in vivo. However, it was not known whether goldfish actually produced the free SN peptide or whether SN directly stimulates LH release from isolated pituitary cells. Using a combination of reverse-phase HPLC and mass spectrometry analysis, we isolated for the first time a 34-amino acid free gfSN peptide from the whole brain. Moreover, Western blot analysis indicated the existence of this peptide in goldfish pituitary. Immunocyto- chemical localization studies revealed the presence of SN immunoreactivity in prolactin cells of rostral pars distalis of the anterior pituitary. Additionally, we found that magnocellular cells of the goldfish preoptic region are highly immunoreactive for SN. These neurons send heavily labeled projections that pass through the pituitary stalk and innervate the neurointermediateand anterior lobes. In static 12-h incubation of dispersed pituitary cells, application of SN antiserum reduced LH levels, whereas 1 and 10 nM gfSN, respectively, induced 2.5-fold (P < 0.001) and 1.9-fold (P < 0.01) increments of LH release into the medium, increases similar to those elicited by 100 nM concentrations of GnRH. LikeGnRH, gfSN elevated intracellular Ca2+ in identified gonadotrophs. Whereas we do not yet know the relative contribution of neural SN or pituitary SN to LH release, we propose that SN could act as a neuroendocrine and/or paracrine factor to regulate LH release from the anterior pituitary. Copyright © 2009 by The Endocrine Society. | ||||||||
Persistent Identifier | http://hdl.handle.net/10722/60699 | ||||||||
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 1.285 | ||||||||
ISI Accession Number ID |
Funding Information: This work was supported by National Sciences and Engineering Research Council Canada (to V. L. T. and J.P.C.), Hong Kong Research Grants Council (RGC) (to A.O.L.W.), and the University of Ottawa Interfaculty Research Program (to V. L. T. and A. B.). | ||||||||
References |
DC Field | Value | Language |
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dc.contributor.author | Zhao, E | en_HK |
dc.contributor.author | Basak, A | en_HK |
dc.contributor.author | Wong, AOL | en_HK |
dc.contributor.author | Ko, W | en_HK |
dc.contributor.author | Chen, A | en_HK |
dc.contributor.author | Lopez, GC | en_HK |
dc.contributor.author | Grey, CL | en_HK |
dc.contributor.author | Canosa, LF | en_HK |
dc.contributor.author | Somoza, GM | en_HK |
dc.contributor.author | Chang, JP | en_HK |
dc.contributor.author | Trudeau, VL | en_HK |
dc.date.accessioned | 2010-05-31T04:16:44Z | - |
dc.date.available | 2010-05-31T04:16:44Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | Endocrinology, 2009, v. 150 n. 5, p. 2273-2282 | en_HK |
dc.identifier.issn | 0013-7227 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/60699 | - |
dc.description.abstract | Secretoneurin (SN) isa 33-to34-aminoacid neuropeptide derived from secretogranin-II, a member of the chromogranin family. We previously synthesized a putative goldfish (gf) SN and demonstrated its ability to stimulate LH release in vivo. However, it was not known whether goldfish actually produced the free SN peptide or whether SN directly stimulates LH release from isolated pituitary cells. Using a combination of reverse-phase HPLC and mass spectrometry analysis, we isolated for the first time a 34-amino acid free gfSN peptide from the whole brain. Moreover, Western blot analysis indicated the existence of this peptide in goldfish pituitary. Immunocyto- chemical localization studies revealed the presence of SN immunoreactivity in prolactin cells of rostral pars distalis of the anterior pituitary. Additionally, we found that magnocellular cells of the goldfish preoptic region are highly immunoreactive for SN. These neurons send heavily labeled projections that pass through the pituitary stalk and innervate the neurointermediateand anterior lobes. In static 12-h incubation of dispersed pituitary cells, application of SN antiserum reduced LH levels, whereas 1 and 10 nM gfSN, respectively, induced 2.5-fold (P < 0.001) and 1.9-fold (P < 0.01) increments of LH release into the medium, increases similar to those elicited by 100 nM concentrations of GnRH. LikeGnRH, gfSN elevated intracellular Ca2+ in identified gonadotrophs. Whereas we do not yet know the relative contribution of neural SN or pituitary SN to LH release, we propose that SN could act as a neuroendocrine and/or paracrine factor to regulate LH release from the anterior pituitary. Copyright © 2009 by The Endocrine Society. | en_HK |
dc.language | eng | en_HK |
dc.publisher | The Endocrine Society. The Journal's web site is located at http://endo.endojournals.org | en_HK |
dc.relation.ispartof | Endocrinology | en_HK |
dc.rights | Endocrinology. Copyright © The Endocrine Society. | en_HK |
dc.title | The secretogranin II-derived peptide secretoneurin stimulates luteinizing hormone secretion from gonadotrophs | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0013-7227&volume=150&spage=2273&epage=2282&date=2009&atitle=The+secretogranin+II-derived+peptide+secretoneurin+stimulates+luteinizing+hormone+secretion+from+gonadotrophs+ | en_HK |
dc.identifier.email | Wong, AOL: olwong@hkucc.hku.hk | en_HK |
dc.identifier.authority | Wong, AOL=rp00806 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1210/en.2008-1060 | en_HK |
dc.identifier.pmid | 19106223 | - |
dc.identifier.scopus | eid_2-s2.0-66449117778 | en_HK |
dc.identifier.hkuros | 164818 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-66449117778&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 150 | en_HK |
dc.identifier.issue | 5 | en_HK |
dc.identifier.spage | 2273 | en_HK |
dc.identifier.epage | 2282 | en_HK |
dc.identifier.isi | WOS:000265407500032 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Zhao, E=7101850098 | en_HK |
dc.identifier.scopusauthorid | Basak, A=35848065700 | en_HK |
dc.identifier.scopusauthorid | Wong, AOL=7403147570 | en_HK |
dc.identifier.scopusauthorid | Ko, W=7202286890 | en_HK |
dc.identifier.scopusauthorid | Chen, A=26641084700 | en_HK |
dc.identifier.scopusauthorid | Lopez, GC=8507136000 | en_HK |
dc.identifier.scopusauthorid | Grey, CL=23102931800 | en_HK |
dc.identifier.scopusauthorid | Canosa, LF=6601938999 | en_HK |
dc.identifier.scopusauthorid | Somoza, GM=7003791807 | en_HK |
dc.identifier.scopusauthorid | Chang, JP=7601547649 | en_HK |
dc.identifier.scopusauthorid | Trudeau, VL=7006634572 | en_HK |
dc.identifier.issnl | 0013-7227 | - |