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- Publisher Website: 10.1093/jxb/ern241
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- PMID: 18836139
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Article: Ethylene- and pathogen-inducible Arabidopsis acyl-CoA-binding protein 4 interacts with an ethylene-responsive element binding protein
Title | Ethylene- and pathogen-inducible Arabidopsis acyl-CoA-binding protein 4 interacts with an ethylene-responsive element binding protein | ||||||||
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Authors | |||||||||
Keywords | Acyl-CoA-binding protein Ethylene Pathogen Protein-protein interaction | ||||||||
Issue Date | 2008 | ||||||||
Publisher | Oxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/ | ||||||||
Citation | Journal Of Experimental Botany, 2008, v. 59 n. 14, p. 3997-4006 How to Cite? | ||||||||
Abstract | Six genes encode proteins with acyl-CoA-binding domains in Arabidopsis thaliana. They are the small 10-kDa cytosolic acyl-CoA-binding protein (ACBP), membrane-associated ACBP1 and ACBP2, extracellularly-targeted ACBP3, and kelch-motif containing ACBP4 and ACBP5. Here, the interaction of ACBP4 with an A. thaliana ethylene-responsive element binding protein (AtEBP), identified in a yeast two-hybrid screen, was confirmed by co-immunoprecipitation. The subcellular localization of ACBP4 and AtEBP, was addressed using an ACBP4:DsRed red fluorescent protein fusion and a green fluorescent protein (GFP):AtEBP fusion. Transient expression of these autofluoresence-tagged proteins in agroinfiltrated tobacco leaves, followed by confocal laser scanning microscopy, indicated their co-localization predominantly at the cytosol which was confirmed by FRET analysis. Immuno-electron microscopy on Arabidopsis sections not only localized ACBP4 to the cytosol but also to the periphery of the nucleus upon closer examination, perhaps as a result of its interaction with AtEBP. Furthermore, the expression of ACBP4 and AtEBP in Northern blot analyses was induced by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, methyl jasmonate treatments, and Botrytis cinerea infection, suggesting that the interaction of ACBP4 and AtEBP may be related to AtEBP-mediated defence possibly via ethylene and/or jasmonate signalling. © 2008 The Author(s). | ||||||||
Persistent Identifier | http://hdl.handle.net/10722/60637 | ||||||||
ISSN | 2023 Impact Factor: 5.6 2023 SCImago Journal Rankings: 1.739 | ||||||||
ISI Accession Number ID |
Funding Information: We thank WL Crosby (Plant Biotechnology Institute, NRC, Canada) for provision of the yeast two-hybrid system; MM Goodin (University of California, Berkeley) for provision of vectors in the construction of autofluorescent protein fusions; and GSW Tsao (The University of Hong Kong) for provision of the Zeiss LSM510 META. This work was supported by the Research Grants Council of the Hong Kong Special Administrative Region, China (projects HKU7314/04M and HKU7504/05M). SX was supported by a postgraduate studentship from the University of Hong Kong and HY Li by the Croucher Foundation. | ||||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, HY | en_HK |
dc.contributor.author | Xiao, S | en_HK |
dc.contributor.author | Chye, ML | en_HK |
dc.date.accessioned | 2010-05-31T04:15:35Z | - |
dc.date.available | 2010-05-31T04:15:35Z | - |
dc.date.issued | 2008 | en_HK |
dc.identifier.citation | Journal Of Experimental Botany, 2008, v. 59 n. 14, p. 3997-4006 | en_HK |
dc.identifier.issn | 0022-0957 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/60637 | - |
dc.description.abstract | Six genes encode proteins with acyl-CoA-binding domains in Arabidopsis thaliana. They are the small 10-kDa cytosolic acyl-CoA-binding protein (ACBP), membrane-associated ACBP1 and ACBP2, extracellularly-targeted ACBP3, and kelch-motif containing ACBP4 and ACBP5. Here, the interaction of ACBP4 with an A. thaliana ethylene-responsive element binding protein (AtEBP), identified in a yeast two-hybrid screen, was confirmed by co-immunoprecipitation. The subcellular localization of ACBP4 and AtEBP, was addressed using an ACBP4:DsRed red fluorescent protein fusion and a green fluorescent protein (GFP):AtEBP fusion. Transient expression of these autofluoresence-tagged proteins in agroinfiltrated tobacco leaves, followed by confocal laser scanning microscopy, indicated their co-localization predominantly at the cytosol which was confirmed by FRET analysis. Immuno-electron microscopy on Arabidopsis sections not only localized ACBP4 to the cytosol but also to the periphery of the nucleus upon closer examination, perhaps as a result of its interaction with AtEBP. Furthermore, the expression of ACBP4 and AtEBP in Northern blot analyses was induced by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, methyl jasmonate treatments, and Botrytis cinerea infection, suggesting that the interaction of ACBP4 and AtEBP may be related to AtEBP-mediated defence possibly via ethylene and/or jasmonate signalling. © 2008 The Author(s). | en_HK |
dc.language | eng | en_HK |
dc.publisher | Oxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/ | en_HK |
dc.relation.ispartof | Journal of Experimental Botany | en_HK |
dc.rights | Journal of Experimental Botany. Copyright © Oxford University Press. | en_HK |
dc.subject | Acyl-CoA-binding protein | en_HK |
dc.subject | Ethylene | en_HK |
dc.subject | Pathogen | en_HK |
dc.subject | Protein-protein interaction | en_HK |
dc.title | Ethylene- and pathogen-inducible Arabidopsis acyl-CoA-binding protein 4 interacts with an ethylene-responsive element binding protein | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-0957&volume=59&spage=3997&epage=4006&date=2008&atitle=Ethylene-and+pathogen-inducible+Arabidopsis+acyl-CoA-binding+protein+4+interacts+with+an+ethylene-responsive+element+binding+protein | en_HK |
dc.identifier.email | Xiao, S: xiaoshi@graduate.hku.hk | en_HK |
dc.identifier.email | Chye, ML: mlchye@hkucc.hku.hk | en_HK |
dc.identifier.authority | Xiao, S=rp00817 | en_HK |
dc.identifier.authority | Chye, ML=rp00687 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1093/jxb/ern241 | en_HK |
dc.identifier.pmid | 18836139 | - |
dc.identifier.scopus | eid_2-s2.0-55249127541 | en_HK |
dc.identifier.hkuros | 154140 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-55249127541&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 59 | en_HK |
dc.identifier.issue | 14 | en_HK |
dc.identifier.spage | 3997 | en_HK |
dc.identifier.epage | 4006 | en_HK |
dc.identifier.isi | WOS:000260556800018 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.scopusauthorid | Li, HY=22953303900 | en_HK |
dc.identifier.scopusauthorid | Xiao, S=7402022635 | en_HK |
dc.identifier.scopusauthorid | Chye, ML=7003905460 | en_HK |
dc.identifier.issnl | 0022-0957 | - |