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- Publisher Website: 10.1107/S1744309109014055
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- PMID: 19407394
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Article: Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9
Title | Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9 |
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Authors | |
Keywords | Coronaviruses Human coronavirus HKU1 Nonstructural proteins |
Issue Date | 2009 |
Publisher | International Union of Crystallography. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291744-3091 |
Citation | Acta Crystallographica Section F: Structural Biology and Crystallization Communications Online, 2009, v. 65 n. 5, p. 526-528 How to Cite? |
Abstract | Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 A resolution and belonged to space group P2(1)2(1)2, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 A, alpha = beta = gamma = 90 degrees and two molecules per asymmetric unit. |
Persistent Identifier | http://hdl.handle.net/10722/59400 |
ISSN | 2014 Impact Factor: 0.524 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wang, W | en_HK |
dc.contributor.author | Wei, L | en_HK |
dc.contributor.author | Yang, A | en_HK |
dc.contributor.author | He, T | en_HK |
dc.contributor.author | Yuen, KY | en_HK |
dc.contributor.author | Chen, C | en_HK |
dc.contributor.author | Rao, Z | en_HK |
dc.date.accessioned | 2010-05-31T03:49:20Z | - |
dc.date.available | 2010-05-31T03:49:20Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | Acta Crystallographica Section F: Structural Biology and Crystallization Communications Online, 2009, v. 65 n. 5, p. 526-528 | en_HK |
dc.identifier.issn | 1744-3091 | - |
dc.identifier.uri | http://hdl.handle.net/10722/59400 | - |
dc.description.abstract | Human coronavirus HKU1 (HCoV-HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single-stranded DNA/RNA-binding properties. The gene that encodes HCoV-HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 A resolution and belonged to space group P2(1)2(1)2, with unit-cell parameters a = 83.5, b = 88.4, c = 31.2 A, alpha = beta = gamma = 90 degrees and two molecules per asymmetric unit. | - |
dc.language | eng | en_HK |
dc.publisher | International Union of Crystallography. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/%28ISSN%291744-3091 | - |
dc.relation.ispartof | Acta Crystallographica Section F: Structural Biology and Crystallization Communications Online | en_HK |
dc.subject | Coronaviruses | - |
dc.subject | Human coronavirus HKU1 | - |
dc.subject | Nonstructural proteins | - |
dc.subject.mesh | Crystallography, X-Ray | - |
dc.subject.mesh | Gene Expression | - |
dc.subject.mesh | RNA-Binding Proteins - chemistry - genetics - metabolism | - |
dc.subject.mesh | SARS Virus - chemistry - genetics - metabolism | - |
dc.subject.mesh | Viral Proteins - chemistry - genetics - metabolism | - |
dc.title | Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9 | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Yuen, KY: kyyuen@hkucc.hku.hk | en_HK |
dc.identifier.email | Chen, C: chench@mail.xtal.tsinghua.edu.cn | - |
dc.identifier.authority | Yuen, KY=rp00366 | en_HK |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1107/S1744309109014055 | - |
dc.identifier.pmid | 19407394 | - |
dc.identifier.pmcid | PMC2675602 | - |
dc.identifier.scopus | eid_2-s2.0-67249102368 | - |
dc.identifier.hkuros | 167084 | en_HK |
dc.identifier.volume | 65 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 526 | - |
dc.identifier.epage | 528 | - |
dc.identifier.isi | WOS:000265639600025 | - |
dc.publisher.place | United States | - |
dc.identifier.citeulike | 4441790 | - |
dc.identifier.issnl | 1744-3091 | - |