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- Publisher Website: 10.1016/j.neuro.2008.11.001
- Scopus: eid_2-s2.0-58749087320
- PMID: 19056420
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Article: Effects of all-trans-retinoic acid on human SH-SY5Y neuroblastoma as in vitro model in neurotoxicity research
Title | Effects of all-trans-retinoic acid on human SH-SY5Y neuroblastoma as in vitro model in neurotoxicity research | ||||||||
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Authors | |||||||||
Keywords | 6-Hydroxydopamine Differentiation MPP+ Neuroblastoma Neurotoxicity Retinoic acid | ||||||||
Issue Date | 2009 | ||||||||
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuro | ||||||||
Citation | Neurotoxicology, 2009, v. 30 n. 1, p. 127-135 How to Cite? | ||||||||
Abstract | Human neuroblastoma SH-SY5Y is a dopaminergic neuronal cell line which has been used as an in vitro model for neurotoxicity experiments. Although the neuroblastoma is usually differentiated by all-trans-retinoic acid (RA), both RA-differentiated and undifferentiated SH-SY5Y cells have been used in neuroscience research. However, the changes in neuronal properties triggered by RA as well as the subsequent responsiveness to neurotoxins have not been comprehensively studied. Therefore, we aim to re-evaluate the differentiation property of RA on this cell line. We hypothesize that modulation of signaling pathways and neuronal properties during RA-mediated differentiation in SH-SY5Y cells can affect their susceptibility to neurotoxins. The differentiation property of RA was confirmed by showing an extensive outgrowth of neurites, increased expressions of neuronal nuclei, neuron specific enolase, synaptophysin and synaptic associated protein-97, and decreased expression of inhibitor of differentiation-1. While undifferentiated SH-SY5Y cells were susceptible to 6-OHDA and MPP+, RA-differentiation conferred SH-SY5Y cells higher tolerance, potentially by up-regulating survival signaling, including Akt pathway as inhibition of Akt removed RA-induced neuroprotection against 6-OHDA. As a result, the real toxicity cannot be revealed in RA-differentiated cells. Therefore, undifferentiated SH-SY5Y is more appropriate for studying neurotoxicity or neuroprotection in experimental Parkinson's disease research. © 2008 Elsevier Inc. All rights reserved. | ||||||||
Persistent Identifier | http://hdl.handle.net/10722/58226 | ||||||||
ISSN | 2021 Impact Factor: 4.398 2020 SCImago Journal Rankings: 1.060 | ||||||||
ISI Accession Number ID |
Funding Information: This work is supported by competitive earmarked research grant (7552/06M) and NSFC/RGC Joint Research Scheme (N-HKU 707/07 M) from Research Grant Council, Hong Kong, University Strategic Research Theme on Drug Discovery from The University of Hong Kong, and HKU Seed Funding for Basic Research (200711159028). | ||||||||
References | |||||||||
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DC Field | Value | Language |
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dc.contributor.author | Cheung, YT | en_HK |
dc.contributor.author | Lau, WKW | en_HK |
dc.contributor.author | Yu, MS | en_HK |
dc.contributor.author | Lai, CSW | en_HK |
dc.contributor.author | Yeung, SC | en_HK |
dc.contributor.author | So, KF | en_HK |
dc.contributor.author | Chang, RCC | en_HK |
dc.date.accessioned | 2010-05-31T03:26:11Z | - |
dc.date.available | 2010-05-31T03:26:11Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | Neurotoxicology, 2009, v. 30 n. 1, p. 127-135 | en_HK |
dc.identifier.issn | 0161-813X | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/58226 | - |
dc.description.abstract | Human neuroblastoma SH-SY5Y is a dopaminergic neuronal cell line which has been used as an in vitro model for neurotoxicity experiments. Although the neuroblastoma is usually differentiated by all-trans-retinoic acid (RA), both RA-differentiated and undifferentiated SH-SY5Y cells have been used in neuroscience research. However, the changes in neuronal properties triggered by RA as well as the subsequent responsiveness to neurotoxins have not been comprehensively studied. Therefore, we aim to re-evaluate the differentiation property of RA on this cell line. We hypothesize that modulation of signaling pathways and neuronal properties during RA-mediated differentiation in SH-SY5Y cells can affect their susceptibility to neurotoxins. The differentiation property of RA was confirmed by showing an extensive outgrowth of neurites, increased expressions of neuronal nuclei, neuron specific enolase, synaptophysin and synaptic associated protein-97, and decreased expression of inhibitor of differentiation-1. While undifferentiated SH-SY5Y cells were susceptible to 6-OHDA and MPP+, RA-differentiation conferred SH-SY5Y cells higher tolerance, potentially by up-regulating survival signaling, including Akt pathway as inhibition of Akt removed RA-induced neuroprotection against 6-OHDA. As a result, the real toxicity cannot be revealed in RA-differentiated cells. Therefore, undifferentiated SH-SY5Y is more appropriate for studying neurotoxicity or neuroprotection in experimental Parkinson's disease research. © 2008 Elsevier Inc. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuro | en_HK |
dc.relation.ispartof | NeuroToxicology | en_HK |
dc.rights | NeuroToxicology. Copyright © Elsevier BV. | en_HK |
dc.subject | 6-Hydroxydopamine | - |
dc.subject | Differentiation | - |
dc.subject | MPP+ | - |
dc.subject | Neuroblastoma | - |
dc.subject | Neurotoxicity | - |
dc.subject | Retinoic acid | - |
dc.subject.mesh | 1-Methyl-4-phenylpyridinium - toxicity | en_HK |
dc.subject.mesh | Biological Markers - analysis | en_HK |
dc.subject.mesh | Cell Differentiation - drug effects | en_HK |
dc.subject.mesh | Cell Line, Tumor | en_HK |
dc.subject.mesh | Cells, Cultured | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Models, Neurological | en_HK |
dc.subject.mesh | Neuroblastoma | en_HK |
dc.subject.mesh | Neurons - drug effects | en_HK |
dc.subject.mesh | Oxidopamine - toxicity | en_HK |
dc.subject.mesh | Reactive Oxygen Species - metabolism | en_HK |
dc.subject.mesh | Signal Transduction | en_HK |
dc.subject.mesh | Tretinoin - pharmacology | en_HK |
dc.title | Effects of all-trans-retinoic acid on human SH-SY5Y neuroblastoma as in vitro model in neurotoxicity research | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0161-813X&volume=30&spage=127&epage=135&date=2009&atitle=Effects+of+all-trans-retinoic+acid+on+human+SH-SY5Y+neuroblastoma+as+in+vitro+model+in+neurotoxicity+research | en_HK |
dc.identifier.email | So, KF:hrmaskf@hkucc.hku.hk | en_HK |
dc.identifier.email | Chang, RCC:rccchang@hkucc.hku.hk | en_HK |
dc.identifier.authority | So, KF=rp00329 | en_HK |
dc.identifier.authority | Chang, RCC=rp00470 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.neuro.2008.11.001 | en_HK |
dc.identifier.pmid | 19056420 | - |
dc.identifier.scopus | eid_2-s2.0-58749087320 | en_HK |
dc.identifier.hkuros | 154884 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-58749087320&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 30 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 127 | en_HK |
dc.identifier.epage | 135 | en_HK |
dc.identifier.isi | WOS:000263047400017 | - |
dc.publisher.place | Netherlands | en_HK |
dc.relation.project | Investigating the impact of oligomeric β-amyloid peptide on RNA-trifficking in neurons. Implication on local protein translation control in neurons. | - |
dc.identifier.scopusauthorid | Cheung, YT=16678256500 | en_HK |
dc.identifier.scopusauthorid | Lau, WKW=35292424400 | en_HK |
dc.identifier.scopusauthorid | Yu, MS=35346047600 | en_HK |
dc.identifier.scopusauthorid | Lai, CSW=26022547000 | en_HK |
dc.identifier.scopusauthorid | Yeung, SC=25923636600 | en_HK |
dc.identifier.scopusauthorid | So, KF=34668391300 | en_HK |
dc.identifier.scopusauthorid | Chang, RCC=7403713410 | en_HK |
dc.identifier.issnl | 0161-813X | - |