File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Human parainfluenza virus 4 outbreak and the role of diagnostic tests

TitleHuman parainfluenza virus 4 outbreak and the role of diagnostic tests
Authors
Issue Date2005
PublisherAmerican Society for Microbiology.
Citation
Journal of Clinical Microbiology, 2005, v. 43 n. 9, p. 4515-4521 How to Cite?
AbstractOwing to the difficulties in isolating the virus and the lack of routine surveillance, the clinical significance of human parainfluenza virus 4 (HPIV-4) is less well defined than that of the other human parainfluenza viruses. We describe the first outbreak of HPIV-4 infection in a developmental disabilities unit, involving 38 institutionalized children and three staff members, during a 3-week period in autumn 2004. Most subjects had upper respiratory tract infections (URTI), while lower respiratory tract infections (LRTI) occurred in three children (7%), one complicated by respiratory failure requiring ventilation support. All patients recovered. Nasopharyngeal aspirates tested for HPIV-4 were positive by reverse transcriptase PCR (RT-PCR) in all 41 cases (100%), by direct immunofluorescence in 29 of 39 tested cases (74%), and by cell cultures in 6 of 37 cases (16%), and serum was positive for antibodies against HPIV-4 in all 35 cases (100%) with serum samples available. In addition, RT-PCR detected HPIV-4 in four children (three LRTI and one URTI) out of 115 patients with community-acquired respiratory tract infection. Molecular analysis of the 1,198-bp phosphoprotein sequences showed that HPIV-4 isolates among the cases were genetically similar, whereas the community controls were more genetically distant, supporting nosocomial transmission of a single HPIV-4 genotype during the outbreak. Moreover, the HPIV-4 causing the outbreak is more closely related to HPIV-4A than HPIV-4B. HPIV-4 may be an important cause of more severe respiratory illness in children. The present RT-PCR assay is a sensitive, specific, and rapid method for the diagnosing HPIV-4 infection. To better define the epidemiology and clinical spectrum of disease of HPIV-4 infections, HPIV-4 should be included in the routine panels of respiratory virus detection on respiratory specimens. Copyright © 2005, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/49171
ISSN
2023 Impact Factor: 6.1
2023 SCImago Journal Rankings: 1.653
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLau, SKPen_HK
dc.contributor.authorTo, WKen_HK
dc.contributor.authorTse, PWTen_HK
dc.contributor.authorChan, AKHen_HK
dc.contributor.authorWoo, PCYen_HK
dc.contributor.authorTsoi, HWen_HK
dc.contributor.authorLeung, AFYen_HK
dc.contributor.authorLi, KSMen_HK
dc.contributor.authorChan, PKSen_HK
dc.contributor.authorLim, WWLen_HK
dc.contributor.authorYung, RWHen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorYuen, KYen_HK
dc.date.accessioned2008-06-12T06:36:00Z-
dc.date.available2008-06-12T06:36:00Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal of Clinical Microbiology, 2005, v. 43 n. 9, p. 4515-4521en_HK
dc.identifier.issn0095-1137en_HK
dc.identifier.urihttp://hdl.handle.net/10722/49171-
dc.description.abstractOwing to the difficulties in isolating the virus and the lack of routine surveillance, the clinical significance of human parainfluenza virus 4 (HPIV-4) is less well defined than that of the other human parainfluenza viruses. We describe the first outbreak of HPIV-4 infection in a developmental disabilities unit, involving 38 institutionalized children and three staff members, during a 3-week period in autumn 2004. Most subjects had upper respiratory tract infections (URTI), while lower respiratory tract infections (LRTI) occurred in three children (7%), one complicated by respiratory failure requiring ventilation support. All patients recovered. Nasopharyngeal aspirates tested for HPIV-4 were positive by reverse transcriptase PCR (RT-PCR) in all 41 cases (100%), by direct immunofluorescence in 29 of 39 tested cases (74%), and by cell cultures in 6 of 37 cases (16%), and serum was positive for antibodies against HPIV-4 in all 35 cases (100%) with serum samples available. In addition, RT-PCR detected HPIV-4 in four children (three LRTI and one URTI) out of 115 patients with community-acquired respiratory tract infection. Molecular analysis of the 1,198-bp phosphoprotein sequences showed that HPIV-4 isolates among the cases were genetically similar, whereas the community controls were more genetically distant, supporting nosocomial transmission of a single HPIV-4 genotype during the outbreak. Moreover, the HPIV-4 causing the outbreak is more closely related to HPIV-4A than HPIV-4B. HPIV-4 may be an important cause of more severe respiratory illness in children. The present RT-PCR assay is a sensitive, specific, and rapid method for the diagnosing HPIV-4 infection. To better define the epidemiology and clinical spectrum of disease of HPIV-4 infections, HPIV-4 should be included in the routine panels of respiratory virus detection on respiratory specimens. Copyright © 2005, American Society for Microbiology. All Rights Reserved.en_HK
dc.format.extent388 bytes-
dc.format.mimetypetext/html-
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofJournal of Clinical Microbiologyen_HK
dc.subject.meshCross Infection - diagnosis - epidemiology - virologyen_HK
dc.subject.meshDisease Outbreaksen_HK
dc.subject.meshParainfluenza Virus 4, Human - classification - genetics - isolation & purificationen_HK
dc.subject.meshRubulavirus Infections - diagnosis - epidemiology - virologyen_HK
dc.subject.meshAntibodies, Viral - blooden_HK
dc.titleHuman parainfluenza virus 4 outbreak and the role of diagnostic testsen_HK
dc.typeArticleen_HK
dc.identifier.emailLau, SKP:skplau@hkucc.hku.hken_HK
dc.identifier.emailWoo, PCY:pcywoo@hkucc.hku.hken_HK
dc.identifier.emailTsoi, HW:hwtsoi@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityLau, SKP=rp00486en_HK
dc.identifier.authorityWoo, PCY=rp00430en_HK
dc.identifier.authorityTsoi, HW=rp00439en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_OA_fulltexten_HK
dc.identifier.doi10.1128/JCM.43.9.4515-4521.2005en_HK
dc.identifier.pmid16145100-
dc.identifier.pmcidPMC1234116en_HK
dc.identifier.scopuseid_2-s2.0-24744440562en_HK
dc.identifier.hkuros114674-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-24744440562&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume43en_HK
dc.identifier.issue9en_HK
dc.identifier.spage4515en_HK
dc.identifier.epage4521en_HK
dc.identifier.isiWOS:000232020400031-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLau, SKP=7401596211en_HK
dc.identifier.scopusauthoridTo, WK=7004294514en_HK
dc.identifier.scopusauthoridTse, PWT=7005336881en_HK
dc.identifier.scopusauthoridChan, AKH=8669885400en_HK
dc.identifier.scopusauthoridWoo, PCY=7201801340en_HK
dc.identifier.scopusauthoridTsoi, HW=6603822102en_HK
dc.identifier.scopusauthoridLeung, AFY=8907665900en_HK
dc.identifier.scopusauthoridLi, KSM=24759122500en_HK
dc.identifier.scopusauthoridChan, PKS=7403497792en_HK
dc.identifier.scopusauthoridLim, WWL=7202378267en_HK
dc.identifier.scopusauthoridYung, RWH=7005594277en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.issnl0095-1137-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats