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- Publisher Website: 10.1016/0092-8674(91)90498-N
- Scopus: eid_2-s2.0-0026073070
- PMID: 1705179
- WOS: WOS:A1991EZ47800004
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Article: Expression of the cystic fibrosis gene in non-epithelial invertebrate cells produces a regulated anion conductance
Title | Expression of the cystic fibrosis gene in non-epithelial invertebrate cells produces a regulated anion conductance |
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Authors | |
Issue Date | 1991 |
Publisher | Cell Press. The Journal's web site is located at http://www.elsevier.com/locate/cell |
Citation | Cell, 1991, v. 64 n. 4, p. 681-691 How to Cite? |
Abstract | The nature of involvement of the cystic fibrosis gene product (CFTR) in epithelial anion transport is not yet understood. We have expressed CFTR in Sf9 insect cells using the baculovirus expression vector system. Reactivity with antibodies against 12 different epitopes spanning the entire sequence suggested that the complete polypeptide chain was synthesized. Immunogold labeling showed localization to both cell-surface and intracellular membranes. Concomitant with CFTR expression, these cells exhibited a new cAMP-stimulated anion permeability. This conductance, monitored both by radioiodide efflux and patch clamping, strongly resembled that present in several CFTR-expressing human epithelial cells. These findings demonstrate that CFTR can function in heterologous nonepithelial cells and lend support to the possibility that CFTR may itself be a regulated anion channel. |
Persistent Identifier | http://hdl.handle.net/10722/44244 |
ISSN | 2023 Impact Factor: 45.5 2023 SCImago Journal Rankings: 24.342 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kartner, N | en_HK |
dc.contributor.author | Hanrahan, JW | en_HK |
dc.contributor.author | Jensen, TJ | en_HK |
dc.contributor.author | Naismith, AL | en_HK |
dc.contributor.author | Shizhang, S | en_HK |
dc.contributor.author | Ackerley, CA | en_HK |
dc.contributor.author | Reyes, EF | en_HK |
dc.contributor.author | Tsui, LC | en_HK |
dc.contributor.author | Rommens, JM | en_HK |
dc.contributor.author | Bear, CE | en_HK |
dc.contributor.author | Riordan, JR | en_HK |
dc.date.accessioned | 2007-09-12T03:49:46Z | - |
dc.date.available | 2007-09-12T03:49:46Z | - |
dc.date.issued | 1991 | en_HK |
dc.identifier.citation | Cell, 1991, v. 64 n. 4, p. 681-691 | en_HK |
dc.identifier.issn | 0092-8674 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/44244 | - |
dc.description.abstract | The nature of involvement of the cystic fibrosis gene product (CFTR) in epithelial anion transport is not yet understood. We have expressed CFTR in Sf9 insect cells using the baculovirus expression vector system. Reactivity with antibodies against 12 different epitopes spanning the entire sequence suggested that the complete polypeptide chain was synthesized. Immunogold labeling showed localization to both cell-surface and intracellular membranes. Concomitant with CFTR expression, these cells exhibited a new cAMP-stimulated anion permeability. This conductance, monitored both by radioiodide efflux and patch clamping, strongly resembled that present in several CFTR-expressing human epithelial cells. These findings demonstrate that CFTR can function in heterologous nonepithelial cells and lend support to the possibility that CFTR may itself be a regulated anion channel. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Cell Press. The Journal's web site is located at http://www.elsevier.com/locate/cell | en_HK |
dc.relation.ispartof | Cell | en_HK |
dc.rights | This paper has been submitted to Cancer Cell for consideration | en_HK |
dc.subject.mesh | Baculoviridae - genetics | en_HK |
dc.subject.mesh | Cell membrane - physiology - ultrastructure | en_HK |
dc.subject.mesh | Cyclic amp - pharmacology | en_HK |
dc.subject.mesh | Cystic fibrosis - genetics | en_HK |
dc.subject.mesh | Ion channels - genetics - physiology | en_HK |
dc.title | Expression of the cystic fibrosis gene in non-epithelial invertebrate cells produces a regulated anion conductance | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0092-8674&volume=64&issue=4&spage=681&epage=691&date=1991&atitle=Expression+of+the+cystic+fibrosis+gene+in+non-epithelial+invertebrate+cells+produces+a+regulated+anion+conductance | en_HK |
dc.identifier.email | Tsui, LC: tsuilc@hkucc.hku.hk | en_HK |
dc.identifier.authority | Tsui, LC=rp00058 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | en_HK |
dc.identifier.doi | 10.1016/0092-8674(91)90498-N | - |
dc.identifier.pmid | 1705179 | - |
dc.identifier.scopus | eid_2-s2.0-0026073070 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0026073070&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 64 | en_HK |
dc.identifier.issue | 4 | en_HK |
dc.identifier.spage | 681 | en_HK |
dc.identifier.epage | 691 | en_HK |
dc.identifier.isi | WOS:A1991EZ47800004 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.scopusauthorid | Kartner, N=6701620107 | en_HK |
dc.identifier.scopusauthorid | Hanrahan, JW=7103386837 | en_HK |
dc.identifier.scopusauthorid | Jensen, TJ=7401539543 | en_HK |
dc.identifier.scopusauthorid | Naismith, AL=6602332581 | en_HK |
dc.identifier.scopusauthorid | Shizhang, S=6504433612 | en_HK |
dc.identifier.scopusauthorid | Ackerley, CA=7006092057 | en_HK |
dc.identifier.scopusauthorid | Reyes, EF=7102192268 | en_HK |
dc.identifier.scopusauthorid | Tsui, LC=7102754167 | en_HK |
dc.identifier.scopusauthorid | Rommens, JM=7006884140 | en_HK |
dc.identifier.scopusauthorid | Bear, CE=7006718679 | en_HK |
dc.identifier.scopusauthorid | Riordan, JR=7202229758 | en_HK |
dc.identifier.issnl | 0092-8674 | - |