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- Publisher Website: 10.1136/jcp.48.9.861
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- PMID: 7490322
- WOS: WOS:A1995RU00800015
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Article: Comparison of haemoglobin H inclusion bodies with embryonic ζ globin in screening for α thalassaemia
Title | Comparison of haemoglobin H inclusion bodies with embryonic ζ globin in screening for α thalassaemia |
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Authors | |
Keywords | α thalassaemia ζ chains HbH inclusion test |
Issue Date | 1995 |
Publisher | B M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/ |
Citation | Journal Of Clinical Pathology, 1995, v. 48 n. 9, p. 861-864 How to Cite? |
Abstract | Aims - To compare the haemoglobin (Hb) H inclusion test with immunocytochemical detection of embryonic ζ chains in screening for a thalassaemia. Methods - Blood samples from 115 patients with relevant clinical history and hypochromic microcytic indexes were screened using the HbH inclusion test and the Variant Hemoglobin Testing System (BioRad, Hercules, CA, USA). Results - The HbH inclusion test was positive in 61 of 115 cases, three of whom had HbH disease confirmed by electrophoresis. The remaining 58 had α thalassaemia 1. All three HbH cases and 56 of 58 cases of a thalassaemia 1 expressed embryonic ζ chains, giving a specificity of 96.7%. Fifty four of 115 cases had a negative HbH inclusion test, of whom 50 had β thalassaemia trait and three had iron deficiency. No diagnosis was reached for the remaining patient. Conclusion - The immunocytochemical test is as sensitive as the HbH inclusion test in screening for a thalassaemia. The presence of ζ chains is highly specific for a thalassaemia I incorporating the (--/SEA) deletion. The specificity and simplicity of the immunocytochemical test make it the test of choice in screening for α thalassaemia. |
Persistent Identifier | http://hdl.handle.net/10722/43614 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.934 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chan, LC | en_HK |
dc.contributor.author | So, JCC | en_HK |
dc.contributor.author | Chui, DHK | en_HK |
dc.date.accessioned | 2007-03-23T04:50:21Z | - |
dc.date.available | 2007-03-23T04:50:21Z | - |
dc.date.issued | 1995 | en_HK |
dc.identifier.citation | Journal Of Clinical Pathology, 1995, v. 48 n. 9, p. 861-864 | en_HK |
dc.identifier.issn | 0021-9746 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/43614 | - |
dc.description.abstract | Aims - To compare the haemoglobin (Hb) H inclusion test with immunocytochemical detection of embryonic ζ chains in screening for a thalassaemia. Methods - Blood samples from 115 patients with relevant clinical history and hypochromic microcytic indexes were screened using the HbH inclusion test and the Variant Hemoglobin Testing System (BioRad, Hercules, CA, USA). Results - The HbH inclusion test was positive in 61 of 115 cases, three of whom had HbH disease confirmed by electrophoresis. The remaining 58 had α thalassaemia 1. All three HbH cases and 56 of 58 cases of a thalassaemia 1 expressed embryonic ζ chains, giving a specificity of 96.7%. Fifty four of 115 cases had a negative HbH inclusion test, of whom 50 had β thalassaemia trait and three had iron deficiency. No diagnosis was reached for the remaining patient. Conclusion - The immunocytochemical test is as sensitive as the HbH inclusion test in screening for a thalassaemia. The presence of ζ chains is highly specific for a thalassaemia I incorporating the (--/SEA) deletion. The specificity and simplicity of the immunocytochemical test make it the test of choice in screening for α thalassaemia. | en_HK |
dc.format.extent | 1102840 bytes | - |
dc.format.extent | 26624 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.format.mimetype | application/msword | - |
dc.language | eng | en_HK |
dc.publisher | B M J Publishing Group. The Journal's web site is located at http://jcp.bmjjournals.com/ | en_HK |
dc.relation.ispartof | Journal of Clinical Pathology | en_HK |
dc.rights | Journal of Clinical Pathology. Copyright © B M J Publishing Group. | en_HK |
dc.subject | α thalassaemia | en_HK |
dc.subject | ζ chains | en_HK |
dc.subject | HbH inclusion test | en_HK |
dc.subject.mesh | Erythrocytes - ultrastructure | en_HK |
dc.subject.mesh | Fluorescent antibody technique | en_HK |
dc.subject.mesh | Globins - analysis | en_HK |
dc.subject.mesh | Inclusion bodies - pathology | en_HK |
dc.subject.mesh | Alpha-thalassemia - blood - diagnosis - genetics | en_HK |
dc.title | Comparison of haemoglobin H inclusion bodies with embryonic ζ globin in screening for α thalassaemia | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9746&volume=48&issue=9&spage=861&epage=864&date=1995&atitle=Comparison+of+haemoglobin+H+inclusion+bodies+with+embryonic+zeta+globin+in+screening+for+alpha+thalassaemia | en_HK |
dc.identifier.email | Chan, LC:chanlc@hkucc.hku.hk | en_HK |
dc.identifier.email | So, JCC:scc@pathology.hku.hk | en_HK |
dc.identifier.authority | Chan, LC=rp00373 | en_HK |
dc.identifier.authority | So, JCC=rp00391 | en_HK |
dc.description.nature | published_or_final_version | en_HK |
dc.identifier.doi | 10.1136/jcp.48.9.861 | - |
dc.identifier.pmid | 7490322 | - |
dc.identifier.pmcid | PMC502877 | - |
dc.identifier.scopus | eid_2-s2.0-0029091409 | en_HK |
dc.identifier.hkuros | 8743 | - |
dc.identifier.volume | 48 | en_HK |
dc.identifier.issue | 9 | en_HK |
dc.identifier.spage | 861 | en_HK |
dc.identifier.epage | 864 | en_HK |
dc.identifier.isi | WOS:A1995RU00800015 | - |
dc.publisher.place | United Kingdom | en_HK |
dc.identifier.issnl | 0021-9746 | - |