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Article: Evaluation of excretory/secretory products of larval Taenia solium as diagnostic antigens for porcine and human cysticercosis

TitleEvaluation of excretory/secretory products of larval Taenia solium as diagnostic antigens for porcine and human cysticercosis
Authors
Ko, RCCNg, TF
Issue Date1998
PublisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=JHL
Citation
Journal of Helminthology, 1998, v. 72 n. 2, p. 147-154 How to Cite?
DOI: http://dx.doi.org/10.1017/S0022149X00016333
AbstractExcretory/secretory antigens (ES) of larval Taenia solium were obtained by maintaining the bladder worms in Medium 199 for 3 days. Analysis by SDS-PAGE showed that ES antigens consisted of at least 19 polypeptides, with M(r) ranging from 14-116 kDa. Analytical isoelectric focusing revealed eight bands with acidic pI. An immunocytolocalization study using the peroxidase method demonstrated the presence of ES epitopes on the tegument of the wall of the spiral canals of bladder worms. The specificity of ES antigens was evaluated by EITB, ELISA and FAST-ELISA using antisera against the common parasites of Chinese pigs and man. ES antigens cross-reacted with the antiserum against larval T. hydatigena of pigs. However, these antigens were generally more specific in diagnosing human cysticercosis. Three host-like molecules with molecular masses 43, 58 and 66 kDa were present in the ES products.
Persistent Identifierhttp://hdl.handle.net/10722/43522
ISSN
0022-149X
2023 Impact Factor: 1.3
2023 SCImago Journal Rankings: 0.406
ISI Accession Number ID
WOS:000074938200009

 

DC FieldValueLanguage
dc.contributor.authorKo, RCCen_HK
dc.contributor.authorNg, TFen_HK
dc.date.accessioned2007-03-23T04:47:53Z-
dc.date.available2007-03-23T04:47:53Z-
dc.date.issued1998en_HK
dc.identifier.citationJournal of Helminthology, 1998, v. 72 n. 2, p. 147-154en_HK
dc.identifier.issn0022-149Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/43522-
dc.description.abstractExcretory/secretory antigens (ES) of larval Taenia solium were obtained by maintaining the bladder worms in Medium 199 for 3 days. Analysis by SDS-PAGE showed that ES antigens consisted of at least 19 polypeptides, with M(r) ranging from 14-116 kDa. Analytical isoelectric focusing revealed eight bands with acidic pI. An immunocytolocalization study using the peroxidase method demonstrated the presence of ES epitopes on the tegument of the wall of the spiral canals of bladder worms. The specificity of ES antigens was evaluated by EITB, ELISA and FAST-ELISA using antisera against the common parasites of Chinese pigs and man. ES antigens cross-reacted with the antiserum against larval T. hydatigena of pigs. However, these antigens were generally more specific in diagnosing human cysticercosis. Three host-like molecules with molecular masses 43, 58 and 66 kDa were present in the ES products.en_HK
dc.format.extent1251951 bytes-
dc.format.extent25600 bytes-
dc.format.mimetypeapplication/pdf-
dc.format.mimetypeapplication/msword-
dc.languageengen_HK
dc.publisherCambridge University Press. The Journal's web site is located at http://journals.cambridge.org/action/displayJournal?jid=JHLen_HK
dc.relation.ispartofJournal of Helminthology-
dc.rightsJournal of Helminthology. Copyright © Cambridge University Press.en_HK
dc.subject.meshCysticercosis - diagnosisen_HK
dc.subject.meshAntigens, helminth - analysisen_HK
dc.subject.meshIsoelectric focusingen_HK
dc.subject.meshSensitivity and specificityen_HK
dc.subject.meshTaenia - chemistry - immunologyen_HK
dc.titleEvaluation of excretory/secretory products of larval Taenia solium as diagnostic antigens for porcine and human cysticercosisen_HK
dc.typeArticleen_HK
dc.description.naturepublished_or_final_versionen_HK
dc.identifier.doi10.1017/S0022149X00016333-
dc.identifier.pmid9687596-
dc.identifier.scopuseid_2-s2.0-0031818872-
dc.identifier.hkuros35265-
dc.identifier.isiWOS:000074938200009-
dc.identifier.issnl0022-149X-

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