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Article: Protocol to track the biosynthesis of cholesterol in cultured HCC cells using 13C compound-specific stable isotopic tracers

TitleProtocol to track the biosynthesis of cholesterol in cultured HCC cells using 13C compound-specific stable isotopic tracers
Authors
Cybulski, JDLeung, KSLeung, CONBaker, DMLee, TKW
KeywordsCancer
Cell Culture
Chemistry
Metabolism
Issue Date15-Sep-2023
PublisherCell Press
Citation
STAR Protocols, 2023, v. 4, n. 3 How to Cite?
DOI: http://dx.doi.org/10.1016/j.xpro.2023.102506
Abstract

Cholesterol biosynthesis supports proliferation and drives resistance to tyrosine kinase inhibitor (TKI) therapy in hepatocellular carcinoma (HCC). Here, we present a protocol for using stable isotopic tracers to track the biosynthesis of cholesterol in cultured HCC cells. We describe steps for cell preparation, incubation, separation, and homogenization. We then detail lipid extraction and compound-specific isotope analysis for comparing and quantifying cholesterol synthesis between TKI-resistant HCC cells and their mock counterparts. This protocol can be expanded for use with other shorter-chained lipids.


Persistent Identifierhttp://hdl.handle.net/10722/357094
ISI Accession Number ID
WOS:001059478600001

 

DC FieldValueLanguage
dc.contributor.authorCybulski, JD-
dc.contributor.authorLeung, KS-
dc.contributor.authorLeung, CON-
dc.contributor.authorBaker, DM-
dc.contributor.authorLee, TKW -
dc.date.accessioned2025-06-23T08:53:20Z-
dc.date.available2025-06-23T08:53:20Z-
dc.date.issued2023-09-15-
dc.identifier.citationSTAR Protocols, 2023, v. 4, n. 3-
dc.identifier.urihttp://hdl.handle.net/10722/357094-
dc.description.abstract<p>Cholesterol biosynthesis supports proliferation and drives resistance to tyrosine kinase inhibitor (TKI) therapy in hepatocellular carcinoma (HCC). Here, we present a protocol for using stable isotopic tracers to track the biosynthesis of cholesterol in cultured HCC cells. We describe steps for cell preparation, incubation, separation, and homogenization. We then detail lipid extraction and compound-specific isotope analysis for comparing and quantifying cholesterol synthesis between TKI-resistant HCC cells and their mock counterparts. This protocol can be expanded for use with other shorter-chained lipids.</p>-
dc.languageeng-
dc.publisherCell Press-
dc.relation.ispartofSTAR Protocols-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectCancer-
dc.subjectCell Culture-
dc.subjectChemistry-
dc.subjectMetabolism-
dc.titleProtocol to track the biosynthesis of cholesterol in cultured HCC cells using 13C compound-specific stable isotopic tracers-
dc.typeArticle-
dc.identifier.doi10.1016/j.xpro.2023.102506-
dc.identifier.scopuseid_2-s2.0-85167980480-
dc.identifier.volume4-
dc.identifier.issue3-
dc.identifier.eissn2666-1667-
dc.identifier.isiWOS:001059478600001-
dc.identifier.issnl2666-1667-

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