Interrelationship between Ferroptosis and Cuproptosis in the Pathogenesis of Intracranial Haemorrhage (ICH)

Abstract

<p>Objective</p><p>Previous studies have shown the presence of cuproptosis in ICH models and also the interplay between ferroptosis and cuproptosis. However, the relationship between the two in ICH remains unknown. This study aimed to investigate the mechanistic link between ferroptosis and cuproptosis in ICH and explored their potential as therapeutic targets.</p><p>Method:</p><p>The study utilized ICH mouse models and heme-induced injury cell culture models. Cells were treated with hemin to simulate heme-induced injury, and the activation of ferroptosis and cuproptosis was studied. In vivo studies were also carried out. In mouse models, ICH was induced, and ferroptosis and cuproptosis markers were evaluated at different time points. Iron chelators were then used to inhibit ferroptosis, aiming to establish the interrelationship between ferroptosis and cuproptosis both in vitro and in vivo.</p><p>Result:</p><p>The results demonstrated an upregulation of ferroptosis and cuproptosis markers. Moreover, the use of ferroptosis inhibitors decreases both the ferroptosis and cuproptosis markers in vitro. Similarly, the same results were obtained in vivo, showing the relationship between ferroptosis and cuproptosis.</p><p>Conclusion:</p><p>This study provides novel insights into the interplay between ferroptosis and cuproptosis in the pathogenesis of ICH. The findings suggest that targeting the interplay between ferroptosis and cuproptosis may offer a promising therapeutic strategy for ICH.</p>