Fractal-Dependent Growth of Solidlike Condensates

Abstract

<p>The phenomenon of droplet growth occurs in various industrial and natural processes. Recently, the discovery of liquidlike condensates within cells has sparked an increasing interest in understanding their growth behaviors. These condensates exhibit varying material properties that are closely related to many cellular functions and diseases, particularly during the phase transition from liquidlike droplets to solidlike aggregates. However, how the liquid-to-solid phase transition affects the growth of condensates remains largely unknown. In this study, we investigate the growth of peptide-RNA condensates, which behave as either liquidlike droplets or solidlike aggregates depending on the RNA sequences. Dynamic light scattering experiments show that solidlike condensates grow surprisingly faster, with their hydrodynamic diameters increasing over time as dh(t)∼t1/2, contrasting with dh(t)∼t1/3 for liquidlike droplets. By combining theoretical analysis and simulations, we demonstrate that this accelerated growth is caused by the noncoalescence aggregation of solidlike condensates and thus formation of percolated swollen structures with a decreased fractal dimension. Moreover, we demonstrate that the accelerated growth can be slowed down by introducing agents that can revert solidlike condensates back to their liquidlike states, such as urea or specific RNAs. Together, our work reveals a fractal-dependent growth mechanism of condensates, with useful insights for understanding the aging of condensates and modulating their aggregation behaviors in synthetic and biological systems.</p>