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Article: Thyroid Hormone Receptor Agonistic and Antagonistic Activity of Newly Synthesized Dihydroxylated Polybrominated Diphenyl Ethers: An In Vitro and In Silico Coactivator Recruitment Study
Title | Thyroid Hormone Receptor Agonistic and Antagonistic Activity of Newly Synthesized Dihydroxylated Polybrominated Diphenyl Ethers: An In Vitro and In Silico Coactivator Recruitment Study |
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Authors | |
Keywords | agonistic or antagonistic activities coactivator recruitment assay DiOH-PBDEs thyroid hormone receptors |
Issue Date | 11-Apr-2024 |
Publisher | MDPI |
Citation | Toxics, 2024, v. 12, n. 4 How to Cite? |
Abstract | Dihydroxylated polybrominated diphenyl ethers (DiOH-PBDEs) could be the metabolites of PBDEs of some organisms or the natural products of certain marine bacteria and algae. OH-PBDEs may demonstrate binding affinity to thyroid hormone receptors (TRs) and can disrupt the functioning of the systems modulated by TRs. However, the thyroid hormone disruption mechanism of diOH-PBDEs remains elusive due to the absence of diOH-PBDEs standards. This investigation explores the potential disruptive effects of OH/diOH-PBDEs on thyroid hormones via competitive binding and coactivator recruitment with TRα and TRβ. At levels of 5000 nM and 25,000 nM, 6-OH-BDE-47 demonstrated significant recruitment of steroid receptor coactivator (SRC), whereas none of the diOH-PBDEs exhibited SRC recruitment within the range of 0.32–25,000 nM. AutoDock CrankPep (ADCP) simulations suggest that the conformation of SRC and TR–ligand complexes, particularly their interaction with Helix 12, rather than binding affinity, plays a pivotal role in ligand agonistic activity. 6,6′-diOH-BDE-47 displayed antagonistic activity towards both TRα and TRβ, while the antagonism of 3,5-diOH-BDE-100 for TRα and TRβ was concentration-dependent. 3,5-diOH-BDE-17 and 3,5-diOH-BDE-51 exhibited no discernible agonistic or antagonistic activities. Molecular docking analysis revealed that the binding energy of 3,3′,5-triiodo-L-thyronine (T3) surpassed that of OH/diOH-PBDEs. 3,5-diOH-BDE-100 exhibited the highest binding energy, whereas 6,6′-diOH-BDE-47 displayed the lowest. These findings suggest that the structural determinants influencing the agonistic and antagonistic activities of halogen phenols may be more intricate than previously proposed, involving factors beyond high-brominated PBDEs or hydroxyl group and bromine substitutions. It is likely that the agonistic or antagonistic propensities of OH/diOH-PBDEs are instigated by protein conformational changes rather than considerations of binding energy. |
Persistent Identifier | http://hdl.handle.net/10722/350885 |
ISSN | 2023 Impact Factor: 3.9 2023 SCImago Journal Rankings: 0.815 |
DC Field | Value | Language |
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dc.contributor.author | Zhang, Mengtao | - |
dc.contributor.author | Shi, Jianghong | - |
dc.contributor.author | Li, Bing | - |
dc.contributor.author | Ge, Hui | - |
dc.contributor.author | Tao, Huanyu | - |
dc.contributor.author | Zhang, Jiawei | - |
dc.contributor.author | Li, Xiaoyan | - |
dc.contributor.author | Cai, Zongwei | - |
dc.date.accessioned | 2024-11-06T00:30:26Z | - |
dc.date.available | 2024-11-06T00:30:26Z | - |
dc.date.issued | 2024-04-11 | - |
dc.identifier.citation | Toxics, 2024, v. 12, n. 4 | - |
dc.identifier.issn | 2305-6304 | - |
dc.identifier.uri | http://hdl.handle.net/10722/350885 | - |
dc.description.abstract | <p>Dihydroxylated polybrominated diphenyl ethers (DiOH-PBDEs) could be the metabolites of PBDEs of some organisms or the natural products of certain marine bacteria and algae. OH-PBDEs may demonstrate binding affinity to thyroid hormone receptors (TRs) and can disrupt the functioning of the systems modulated by TRs. However, the thyroid hormone disruption mechanism of diOH-PBDEs remains elusive due to the absence of diOH-PBDEs standards. This investigation explores the potential disruptive effects of OH/diOH-PBDEs on thyroid hormones via competitive binding and coactivator recruitment with TRα and TRβ. At levels of 5000 nM and 25,000 nM, 6-OH-BDE-47 demonstrated significant recruitment of steroid receptor coactivator (SRC), whereas none of the diOH-PBDEs exhibited SRC recruitment within the range of 0.32–25,000 nM. AutoDock CrankPep (ADCP) simulations suggest that the conformation of SRC and TR–ligand complexes, particularly their interaction with Helix 12, rather than binding affinity, plays a pivotal role in ligand agonistic activity. 6,6′-diOH-BDE-47 displayed antagonistic activity towards both TRα and TRβ, while the antagonism of 3,5-diOH-BDE-100 for TRα and TRβ was concentration-dependent. 3,5-diOH-BDE-17 and 3,5-diOH-BDE-51 exhibited no discernible agonistic or antagonistic activities. Molecular docking analysis revealed that the binding energy of 3,3′,5-triiodo-L-thyronine (T3) surpassed that of OH/diOH-PBDEs. 3,5-diOH-BDE-100 exhibited the highest binding energy, whereas 6,6′-diOH-BDE-47 displayed the lowest. These findings suggest that the structural determinants influencing the agonistic and antagonistic activities of halogen phenols may be more intricate than previously proposed, involving factors beyond high-brominated PBDEs or hydroxyl group and bromine substitutions. It is likely that the agonistic or antagonistic propensities of OH/diOH-PBDEs are instigated by protein conformational changes rather than considerations of binding energy.</p> | - |
dc.language | eng | - |
dc.publisher | MDPI | - |
dc.relation.ispartof | Toxics | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | agonistic or antagonistic activities | - |
dc.subject | coactivator recruitment assay | - |
dc.subject | DiOH-PBDEs | - |
dc.subject | thyroid hormone receptors | - |
dc.title | Thyroid Hormone Receptor Agonistic and Antagonistic Activity of Newly Synthesized Dihydroxylated Polybrominated Diphenyl Ethers: An In Vitro and In Silico Coactivator Recruitment Study | - |
dc.type | Article | - |
dc.identifier.doi | 10.3390/toxics12040281 | - |
dc.identifier.scopus | eid_2-s2.0-85191728503 | - |
dc.identifier.volume | 12 | - |
dc.identifier.issue | 4 | - |
dc.identifier.eissn | 2305-6304 | - |
dc.identifier.issnl | 2305-6304 | - |