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Article: Characterization of a polysaccharide from Amauroderma rugosum and its proangiogenic activities in vitro and in vivo

TitleCharacterization of a polysaccharide from Amauroderma rugosum and its proangiogenic activities in vitro and in vivo
Authors
KeywordsAmauroderma rugosum
Polysaccharides
Proangiogenesis
Issue Date1-Jun-2024
PublisherElsevier
Citation
International Journal of Biological Macromolecules, 2024, v. 271 How to Cite?
Abstract

Amauroderma rugosum (AR), also known as “Blood Lingzhi” in Chinese, is a basidiomycete belonging to the Ganodermataceae family. Four polysaccharide fractions were systematically isolated and purified from AR. Subsequently, their compositions were examined and analyzed via high-performance gel permeation chromatography (HPGPC), analysis of the monosaccharide composition, Fourier-transform infrared spectroscopy (FT-IR), and 1H nuclear magnetic resonance (NMR). The zebrafish model was then used to screen for proangiogenic activities of polysaccharides by inducing vascular insufficiency with VEGF receptor tyrosine kinase inhibitor II (VRI). The third fraction of AR polysaccharides (PAR-3) demonstrated the most pronounced proangiogenic effects, effectively ameliorating VRI-induced intersegmental vessel deficiency in zebrafish. Concurrently, the mRNA expression levels of vascular endothelial growth factor (VEGF)-A and VEGF receptors were upregulated by PAR-3. Moreover, the proliferation, migration, invasion, and tube formation of human umbilical vein endothelial cells (HUVECs) were also stimulated by PAR-3, consistently demonstrating that PAR-3 possesses favorable proangiogenic properties. The activation of the Akt, ERK1/2, p38 MAPK, and FAK was most likely the underlying mechanism. In conclusion, this study establishes that PAR-3 isolated from Amauroderma rugosum exhibits potential as a bioresource for promoting angiogenesis.


Persistent Identifierhttp://hdl.handle.net/10722/350474
ISSN
2023 Impact Factor: 7.7
2023 SCImago Journal Rankings: 1.245

 

DC FieldValueLanguage
dc.contributor.authorNie, Xin-
dc.contributor.authorLi, Jingjing-
dc.contributor.authorCheng, Yanfen-
dc.contributor.authorRangsinth, Panthakarn-
dc.contributor.authorWu, Xiaoping-
dc.contributor.authorZheng, Chengwen-
dc.contributor.authorShiu, Polly Ho Ting-
dc.contributor.authorLi, Renkai-
dc.contributor.authorXu, Nan-
dc.contributor.authorHe, Yulin-
dc.contributor.authorLau, Benson Wui Man-
dc.contributor.authorSeto, Sai Wang-
dc.contributor.authorZhang, Jinming-
dc.contributor.authorLee, Simon Ming Yuen-
dc.contributor.authorLeung, George Pak Heng-
dc.date.accessioned2024-10-29T00:31:47Z-
dc.date.available2024-10-29T00:31:47Z-
dc.date.issued2024-06-01-
dc.identifier.citationInternational Journal of Biological Macromolecules, 2024, v. 271-
dc.identifier.issn0141-8130-
dc.identifier.urihttp://hdl.handle.net/10722/350474-
dc.description.abstract<p>Amauroderma rugosum (AR), also known as “Blood Lingzhi” in Chinese, is a basidiomycete belonging to the Ganodermataceae family. Four polysaccharide fractions were systematically isolated and purified from AR. Subsequently, their compositions were examined and analyzed via high-performance gel permeation chromatography (HPGPC), analysis of the monosaccharide composition, Fourier-transform infrared spectroscopy (FT-IR), and 1H nuclear magnetic resonance (NMR). The zebrafish model was then used to screen for proangiogenic activities of polysaccharides by inducing vascular insufficiency with VEGF receptor tyrosine kinase inhibitor II (VRI). The third fraction of AR polysaccharides (PAR-3) demonstrated the most pronounced proangiogenic effects, effectively ameliorating VRI-induced intersegmental vessel deficiency in zebrafish. Concurrently, the mRNA expression levels of vascular endothelial growth factor (VEGF)-A and VEGF receptors were upregulated by PAR-3. Moreover, the proliferation, migration, invasion, and tube formation of human umbilical vein endothelial cells (HUVECs) were also stimulated by PAR-3, consistently demonstrating that PAR-3 possesses favorable proangiogenic properties. The activation of the Akt, ERK1/2, p38 MAPK, and FAK was most likely the underlying mechanism. In conclusion, this study establishes that PAR-3 isolated from Amauroderma rugosum exhibits potential as a bioresource for promoting angiogenesis.</p>-
dc.languageeng-
dc.publisherElsevier-
dc.relation.ispartofInternational Journal of Biological Macromolecules-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectAmauroderma rugosum-
dc.subjectPolysaccharides-
dc.subjectProangiogenesis-
dc.titleCharacterization of a polysaccharide from Amauroderma rugosum and its proangiogenic activities in vitro and in vivo -
dc.typeArticle-
dc.identifier.doi10.1016/j.ijbiomac.2024.132533-
dc.identifier.pmid38777026-
dc.identifier.scopuseid_2-s2.0-85194489521-
dc.identifier.volume271-
dc.identifier.eissn1879-0003-
dc.identifier.issnl0141-8130-

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