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Book Chapter: siRNA design and delivery based on carbon nanotubes

TitlesiRNA design and delivery based on carbon nanotubes
Authors
Li, DanyangAl-Jamal, Khuloud T.
KeywordsBinding capacity
Carbon nanotubes
Cationic polymer
N/P ratio
Silencing efficiency
siRNA delivery
Issue Date2021
Citation
Methods in Molecular Biology, 2021, v. 2282, p. 181-193 How to Cite?
DOI: http://dx.doi.org/10.1007/978-1-0716-1298-9_12
AbstractOwing to the unique physical and chemical properties of carbon nanotubes, they have been widely explored as delivery vectors for proteins, and nucleic acid etc. after functionalization. Particularly, the modification of carbon nanotubes suited for the delivery of siRNA has been intensely studied over the past decade. The assay described in this chapter allows for realizable quantification of siRNA binding on carbon nanotube-based materials using gel electrophoresis and silencing by flow cytometry when the siRNA complexes are delivered in vitro.
Persistent Identifierhttp://hdl.handle.net/10722/349557
ISSN
1064-3745
2023 SCImago Journal Rankings: 0.399

 

DC FieldValueLanguage
dc.contributor.authorLi, Danyang-
dc.contributor.authorAl-Jamal, Khuloud T.-
dc.date.accessioned2024-10-17T06:59:19Z-
dc.date.available2024-10-17T06:59:19Z-
dc.date.issued2021-
dc.identifier.citationMethods in Molecular Biology, 2021, v. 2282, p. 181-193-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/349557-
dc.description.abstractOwing to the unique physical and chemical properties of carbon nanotubes, they have been widely explored as delivery vectors for proteins, and nucleic acid etc. after functionalization. Particularly, the modification of carbon nanotubes suited for the delivery of siRNA has been intensely studied over the past decade. The assay described in this chapter allows for realizable quantification of siRNA binding on carbon nanotube-based materials using gel electrophoresis and silencing by flow cytometry when the siRNA complexes are delivered in vitro.-
dc.languageeng-
dc.relation.ispartofMethods in Molecular Biology-
dc.subjectBinding capacity-
dc.subjectCarbon nanotubes-
dc.subjectCationic polymer-
dc.subjectN/P ratio-
dc.subjectSilencing efficiency-
dc.subjectsiRNA delivery-
dc.titlesiRNA design and delivery based on carbon nanotubes-
dc.typeBook_Chapter-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/978-1-0716-1298-9_12-
dc.identifier.pmid33928577-
dc.identifier.scopuseid_2-s2.0-85105181750-
dc.identifier.volume2282-
dc.identifier.spage181-
dc.identifier.epage193-
dc.identifier.eissn1940-6029-

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