File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.3390/mi12010037
- Scopus: eid_2-s2.0-85099506397
Supplementary
-
Citations:
- Scopus: 0
- Appears in Collections:
Article: Development of real-time transendothelial electrical resistance monitoring for an in vitro blood-brain barrier system
Title | Development of real-time transendothelial electrical resistance monitoring for an in vitro blood-brain barrier system |
---|---|
Authors | |
Keywords | 3D cell culture In-situ monitoring TEER Transendothelial electrical resistance |
Issue Date | 2021 |
Citation | Micromachines, 2021, v. 12, n. 1, p. 1-11 How to Cite? |
Abstract | Three-dimensional (3D) cell cultures and organs-on-a-chip have been developed to construct microenvironments that resemble the environment within the human body and to provide a platform that enables clear observation and accurate assessments of cell behavior. However, direct observation of transendothelial electrical resistance (TEER) has been challenging. To improve the efficiency in monitoring the cell development in organs-on-a-chip, in this study, we designed and integrated commercially available TEER measurement electrodes into an in vitro blood-brain barrier (BBB)-on-chip system to quantify TEER variation. Moreover, a flowing culture medium was added to the monolayered cells to simulate the promotion of continuous shear stress on cerebrovascular cells. Compared with static 3D cell culture, the proposed BBB-on-chip integrated with electrodes could measure TEER in a real-time manner over a long period. It also allowed cell growth angle measurement, providing instant reports of cell growth information online. Overall, the results demonstrated that the developed system can aid in the quantification of the continuous cell-pattern variations for future studies in drug testing. |
Persistent Identifier | http://hdl.handle.net/10722/349513 |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tu, Kai Hong | - |
dc.contributor.author | Yu, Ling Shan | - |
dc.contributor.author | Sie, Zong Han | - |
dc.contributor.author | Hsu, Han Yi | - |
dc.contributor.author | Al-Jamal, Khuloud T. | - |
dc.contributor.author | Wang, Julie Tzu Wen | - |
dc.contributor.author | Chiang, Ya Yu | - |
dc.date.accessioned | 2024-10-17T06:59:01Z | - |
dc.date.available | 2024-10-17T06:59:01Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Micromachines, 2021, v. 12, n. 1, p. 1-11 | - |
dc.identifier.uri | http://hdl.handle.net/10722/349513 | - |
dc.description.abstract | Three-dimensional (3D) cell cultures and organs-on-a-chip have been developed to construct microenvironments that resemble the environment within the human body and to provide a platform that enables clear observation and accurate assessments of cell behavior. However, direct observation of transendothelial electrical resistance (TEER) has been challenging. To improve the efficiency in monitoring the cell development in organs-on-a-chip, in this study, we designed and integrated commercially available TEER measurement electrodes into an in vitro blood-brain barrier (BBB)-on-chip system to quantify TEER variation. Moreover, a flowing culture medium was added to the monolayered cells to simulate the promotion of continuous shear stress on cerebrovascular cells. Compared with static 3D cell culture, the proposed BBB-on-chip integrated with electrodes could measure TEER in a real-time manner over a long period. It also allowed cell growth angle measurement, providing instant reports of cell growth information online. Overall, the results demonstrated that the developed system can aid in the quantification of the continuous cell-pattern variations for future studies in drug testing. | - |
dc.language | eng | - |
dc.relation.ispartof | Micromachines | - |
dc.subject | 3D cell culture | - |
dc.subject | In-situ monitoring | - |
dc.subject | TEER | - |
dc.subject | Transendothelial electrical resistance | - |
dc.title | Development of real-time transendothelial electrical resistance monitoring for an in vitro blood-brain barrier system | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.3390/mi12010037 | - |
dc.identifier.scopus | eid_2-s2.0-85099506397 | - |
dc.identifier.volume | 12 | - |
dc.identifier.issue | 1 | - |
dc.identifier.spage | 1 | - |
dc.identifier.epage | 11 | - |
dc.identifier.eissn | 2072-666X | - |